Cdc25a (NM_133571) Rat Tagged ORF Clone

CAT#: RR206300

Cdc25a (Myc-DDK-tagged ORF) - Rat cell division cycle 25 homolog A (S. pombe) (Cdc25a), (10 ug)

Product Images

Circular map for RR206300

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc25a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206300 representing NM_133571
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGGGCCCGGAGCCCCCCCACCGCCGCCGCCTGCTCTTCACTTGCAGCCCCACTCCTGCGCCGC
  AGCCCACGGGGAAGGTGCAGTTTGGCGCGTCACGTGCTGGCGGACTGTCCCCTGTCACCAACCTGACGGT
  CACCATGGACCAGCTGGAAGGGCTGGGCAGTGACTATGAGAAACCAATGGACGTGAGAAATAGCAGCAGT
  CTACAGAGAATGGGCTCCTCTGAATCGACTGATTCAGGTTTCTGTCTAGATTCTCCTGGGCCCTTGGACA
  GTAAAGAAAACCTTGAAATTTCCCTGAGGAGAATAAATTGCCTACCTCAGAAGCTCTTGGGGTGTAGCCC
  AGCGCTAAAGAGGAGCCATTCTGATTCTCTAGACCACGACATCTTTCAACTCATTGACCAGGATGAAAAT
  AAAGAAAATGAAGCATTTGAATTTAAAAAGCCAATAAGACCTGCATCTCGTGGCTGCCTGAATGCTCACG
  TTCACGAGGAAAGTAAGGACCCCTTTACACACAGGCAGAATTCAGCCCCAGCTCGGATGCTGTCTTCAAA
  TGAAAGTGACATTAGTGAATCAGGAAATTTCAGTCCTCTTTTTACACCCCAGTCACCTGTGAAAGCGAGT
  TTGTCTGATGAGGATGATGGCTTCATAGACCTTCTGGATGGAGAGAATCTGAAGAATGATGAGGAGACCC
  CGTCGTGCATGTCAAGCCTCTGGACCGCTCCCCTTGTCATGAGAAGACCTACAAACCTTGCCGATCGATG
  TGGACTGTTTGACTCCCCTTCCCCGTGCAGCTCCACCAGCAGCTGCAGCACTCGGGCAGTGAAGAGAGCA
  GACCGATCTCATGAGGAGTCTCCTCGAGGTACAAAGCGGAGGAAGAGCAGTGAGGCCAGTCCAGTGAAGG
  CAGATGTTCCGGAGCCAACGCAGCTTCCACACCAGTCTCTCTCCCTGACATCTTTCCCCAAAGGAACCAT
  TGAGAACATTTTCCACAGTGACCCAAGAGACCTTATAGGGGATTTCTCCAAGGGTTACCTCTTTCATACG
  GTCTCTGGGAAGCATCAGGATTTGAAATATATTTCTCCAGAAATTATGGCATCTGTTTTGAATGGCAAGT
  TTGCCAATCTCATTAAAGAGTTTGTTATCATTGACTGCCGATACCCATATGAATATGAAGGAGGGCACAT
  CAAGGGTGCCGTGAACTTGCACATGGAAGAAGAGGTTGAGGAGTTCTTACTCAAGAAACCTATCGTGCCC
  GCTGACGGCAAGCGTGTCATTGTCGTGTTCCACTGTGAGTTCTCCTCTGAGAGAGGCCCTCGGATGTGCC
  GATATGTACGGGAACGAGATAGGCTTGGCAATGAATACCCCAAACTCCACTACCCTGAGCTGTATGTCCT
  GAAGGGGGGATACAAGGAGTTCTTTTTGAAATGCCAGTCTCACTGTGAACCCCCCAGCTACCGACCGATG
  CACCATGAAGACTTTAAAGAAGACCTAAAGAAGTTCCGCACCAAGAGCCGGACCTGGGCAGGGGAGAAGA
  GCAAAAGGGAGATGTACAGTCGCCTGAAGAAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206300 representing NM_133571
  Red=Cloning site Green=Tags(s)
  
  MELGPEPPHRRRLLFTCSPTPAPQPTGKVQFGASRAGGLSPVTNLTVTMDQLEGLGSDYEKPMDVRNSSS
  LQRMGSSESTDSGFCLDSPGPLDSKENLEISLRRINCLPQKLLGCSPALKRSHSDSLDHDIFQLIDQDEN
  KENEAFEFKKPIRPASRGCLNAHVHEESKDPFTHRQNSAPARMLSSNESDISESGNFSPLFTPQSPVKAS
  LSDEDDGFIDLLDGENLKNDEETPSCMSSLWTAPLVMRRPTNLADRCGLFDSPSPCSSTSSCSTRAVKRA
  DRSHEESPRGTKRRKSSEASPVKADVPEPTQLPHQSLSLTSFPKGTIENIFHSDPRDLIGDFSKGYLFHT
  VSGKHQDLKYISPEIMASVLNGKFANLIKEFVIIDCRYPYEYEGGHIKGAVNLHMEEEVEEFLLKKPIVP
  ADGKRVIVVFHCEFSSERGPRMCRYVRERDRLGNEYPKLHYPELYVLKGGYKEFFLKCQSHCEPPSYRPM
  HHEDFKEDLKKFRTKSRTWAGEKSKREMYSRLKKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133571
• ORF Size: 1575 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133571.1, NP_598255.1
• RefSeq Size: 3415 bp
• RefSeq ORF: 1578 bp
• Locus ID: 171102
• UniProt ID: P48965
• Cytogenetics: 8q32
• MW: 59.7 kDa
• Gene Summary: novel phosphatase that mediates the start of the cell cycle; predominantly expressed in late G1, does not fucntion as mitotic regulator [RGD, Feb 2006]