Kdm5b (NM_001107177) Rat Tagged ORF Clone

CAT#: RR206018

Kdm5b (Myc-DDK-tagged ORF) - Rat jumonji, AT rich interactive domain 1B (Jarid1b), (10 ug)

Product Images

Circular map for RR206018

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kdm5b
• Synonyms: Jarid1b; RGD1565602
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206018 representing NM_001107177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGGCCACCGCGCTGCCCCCAGGCCCGCGCCCGGCGCTGCCCCTTGGGGGCCCCGGCCCGCTGG
  GAGAGTTCCTGCCTCCCCCCGAGTGTCCGGTCTTCGAGCCCAGCTGGGAAGAGTTCGCGGACCCCTTCGC
  TTTCATCCACAAGATCCGGCCCATAGCCGAGCAGACTGGGATCTGTAAGGTGCGGCCGCCGCCGGATTGG
  CAGCCACCATTCGCTTGTGATGTTGATAAACTTCATTTTACCCCGCGTATCCAGAGGCTTAATGAGCTGG
  AGGCCCAAACACGTGTAAAATTGAACTTCCTGGACCAGATTGCCAAGTATTGGGAGCTACAGGGAAGTAC
  TCTGAAGATTCCTCATGTGGAGAGAAAGATCTTAGACTTGTTCCAGCTAAATAAGTTAGTTGCAGAGGAA
  GGTGGGTTTGCAGTTGTTTGCAAGGACAGAAAATGGACCAAAATTGCCACCAAGATGGGTTTTGCTCCTG
  GTAAAGCAGTGGGCTCACATATCAGGGGACATTATGAGCGAATCCTCAATCCGTACAATTTGTTCCTATC
  TGGGGACAGCCTGAGGTGTCTTCAGAAGCCTAACCTGACCTCGGACACAAAGGACAAGGAGTACAAACCC
  CATGATATTCCCCAGAGGCAGTCTGTGCAGCCCTCAGAAACCTGCCCTCCGGCCCGCCGAGCAAAGCGCA
  TGAGAGCAGAGGCTATGAATATTAAAATTGAGCCAGAAGAGGCAACAGAAGCCAGGACTCATAACCTGAG
  GCGCCGAATGGGCTGTGCCACTCCAAAACGGGAAAACGAGAAAGAAGTGAAAAGTACCATCAAGCAAGAA
  CCCACCGAGAAGAAAGACTGTGTGTTAGAAAGTGAGAAGGACAAGCCCAAGAGCCGTGCCAAGAAAACCG
  CCACTGCTGTGGACCTGTATGTGTGCCTCTTATGTGGCAGCGGCAATGACGAGGACCGGCTGCTCCTGTG
  TGACGGCTGTGACGACAGTTACCACACCTTCTGCCTGCTTCCCCCTCTCCATGATGTTCCCAAGGGAGAC
  TGGAGGTGTCCTAAGTGTTTGGCTCAAGAGTGTAGTAAGCCACAAGAAGCTTTCGGCTTTGAACAGGCTG
  CAAGGGACTATACACTCCGTACGTTTGGGGAAATGGCAGATGCATTCAAATCTGATTACTTCAACATGCC
  GGTCCATATGGTTCCCACAGAGCTGGTGGAGAAGGAGTTTTGGCGGCTGGTAAGCACTATTGAAGAAGAC
  GTCACCGTGGAGTATGGAGCTGATATTGCTTCAAAAGAGTTTGGCAGTGGCTTTCCTGTTCGAGATGGCA
  AAATCAAGATTTCTCCTGAGGAAGAGGAGTATCTTGATAGTGGCTGGAATTTGAATAACATGCCAGTGAT
  GGAGCAGTCTGTCCTTGCTCACATTACTGCTGATATATGCGGCATGAAACTGCCTTGGTTGTATGTGGGC
  ATGTGCTTTTCTTCCTTCTGTTGGCATATCGAAGACCACTGGAGCTATTCAATCAACTACCTGCACTGGG
  GTGAGCCGAAAACCTGGTATGGAGTTCCAGGATATGCTGCTGAGCAGCTTGAAAACGTAATGAAGAAGCT
  GGCTCCAGAGCTCTTTGTGTCCCAGCCAGACCTCCTCCACCAGCTTGTGACGATCATGAACCCCAATACG
  CTGATGACTCACGAAGTGCCTGTGTATCGAACGAATCAGTGTGCTGGGGAGTTTGTCATTACGTTCCCGA
  GAGCCTACCACAGTGGCTTCAATCAAGGCTTTAATTTTGCTGAGGCAGTTAACTTCTGCACTGTTGATTG
  GCTGCCGTTGGGCCGCCAGTGCGTGGAGCATTATCGCCTGCTGCACCGCTACTGCGTCTTTTCCCATGAT
  GAGATGATATGCAAGATGGCTTCCAAGGCTGATGTGTTAGACGTGGTAGTGGCATCCACTGTTCAGAAGG
  ACATGGCCATCATGATTGAGGATGAAAAGGCTTTAAGGGAAACTGTCCGTAAACTGGGAGTGATTGATTC
  TGAGAGAATGGACTTTGAGCTGTTGCCCGATGATGAGCGGCAGTGTATAAAGTGCAAAACTACGTGCTTC
  ATGTCTGCCATCTCCTGCTCCTGTAAACCTGGCCTGCTTGTTTGCCTGCATCATGTCAAAGAGCTGTGCT
  CCTGTCCCCCTTACAAGTATAACCTGCGGTATAGATACACTCTGGATGATCTCTATCCGATGATGAATGC
  GTTGAAGCTTCGAGCAGAGTCGTACAATGAATGGGCCTTAAATGTGAATGAAGCCTTGGAAGCAAAGATT
  AATAAGAAGAAAAGTCTCGTCAGCTTTAAAGCTTTGATTGAAGAATCAGAAATGAAGAAATTCCCAGACA
  ATGATCTTTTGCGTCACCTTCGCCTAGTTACGCAGGATGCAGAAAAGTGTGCCTCTGTTGCCCAGCAGTT
  GCTTAATGGCAAAAGGCAAACGAGGTATCGATCTGGTGGAGGGAAGTCTCAGAGTCAGCTGACAGTGAAT
  GAGCTTCGGCAGTTCGTGACTCAGCTGTGCGCTCTTCCCTGCGTCCTTAGTCAGACACCACTGCTAAAGG
  ATCTCTTGAATCGTGTGGAAGATTTCCAGCAGCAAAGCCAGAGACTGCTGTCTGAGGAGAGGCCCCGTGC
  TTCTGAGCTTCAGCAGTTGCTGGATGTCAGCTTCACTTTTGATGTTGAACTTCCACAGCTAGCTGAGATG
  CGTATCCGCTTAGAACAGGCCCGTTGGCTAGAAGAGGTTCAGCAGGCTTGCCTGGACTCCAGCTCCCTTT
  CTTTGGATGATATGAGACGACTCATAGACTTAGGGGTGGGGCTAGCTCCATATTCTGCCGTGGAGAAAGC
  TATGGCCCGCCTGCAGGAACTGCTAACAGTGTCAGAGCACTGGGACGACAAAGCCAAGAGTCTCCTCAGA
  GCAAGACCTCGGCATTCCTTGAGTAGCCTTGCTACAGCAGTAAAAGAGATGGAGGAGATCCCCGCCTATC
  TGCCCAATGGTACAGTCCTGAAGGATTCTGTGCAGAAAGCCAGAGACTGGGTTCAGGATGTAGATGCATT
  GCAGGCAGGAGGACGCGTGCCAGTGTTAGAAACACTCATTGAACTTGTCGCAAGAGGCCGATCCATCCCG
  GTACATCTGAATTCTTTACCCAGATTGGAAATGCTAGTATCCGAGGTTCATGCTTGGAAAGAAAGCGCTG
  CTAAAACATTCCTGCTTGAGAATTCGCCATACTCTCTGTTAGAGGTTCTTTGTCCTCGGTGTGACATTGG
  CCTTTTGGGATTGAAAAGGAAGCAGAGAAAGTTGAAGGATCCCTTGCCAAGTGGGAAGAAGAGAAGCACC
  AAGTTAGAGAGTCTGAGTGATCTGGAAAGGGCTCTGACTGAGAGCAAAGAGACGGCGTCAGCTATGGCAA
  CTCTTGGGGAAGCTCGCCTACGAGAGATGGAAGCTTTGCAGTCTCTCAGATTTGCCAATGAAGAGAAATT
  GCTGTCACCTGCCCAAGATGTGGAGACGAAGGTTTGCTTGTGTCAGAAGACTCCAGCCACTCCCATGATT
  CAGTGTGAACTCTGCAGGGATGCTTTCCATACTAGTTGTGTGGCGGTGTCCAGTATTTCACAAAGCTCCC
  GAATCTGGCTTTGTCCTCACTGTCGAAGGTCAGAGAAGCCTCCATTAGAGAAAATTCTGCCTCTGCTGGC
  CTCCCTACAGCGTATAAGAGTTCGTCTTCCTGAAGGGGATGCACTCCGATACATGATTGAAAGAACCGTG
  AACTGGCAACATAGAGCCCAGCAACTGCTTTCTTCAGGGAATCTAAAACTTGTGCAGGACCAAGCGGGTT
  CTGGACTGTTGTTTAGCAGATGGCCAGCCTCAGCAGGAACGGCGTCAGCAGCAGACAAGGTGTCTCAACC
  TCCTGGCACAACATCCTTTTCATTGCCGGAAGACTGGGACAACAGAACCTCATATTTACACTCTCCCTTC
  TCTACCGGTCAGAGTTGTATCCCCCTTCATGGTTTGAGTCCAGAGGTGAATGAATTGCTGATGGAAGCCC
  AGTTGCTCCAGGTGTCCCTCCCTGAAGTCCAGGAGCTTTATCAGACTTTACTTACGAAGCCAAGCTCTGC
  TCAGCAAGCCGACCGAAGCTCACCAGTCAGACCCAGCAGTGAGAAGAACGATTGTCTTCGAGGGAAACGA
  GATGCAATCAACAGTCTTGAGAGAAAACTGAAAAGACGCCCAGAAAGAGAAGGCCTTCCCAGTGAACGGT
  GGGATCGAGTTAAAAAAATGCGGACCCCTCAAAAGAAGAAAATCAAACTGAGTCACCCCAAGGACATGAA
  CAATTTCAAATTAGAAAGAGAGCGTAGCTATGACTTAGTCCGGAATGCTGAAACTCATTCCCTGCCCTCA
  GACACATCCTACTCCGAGCAGGAGGACTCTGAGGATGAAGATGCCATCTGTCCAGCTGTGAGCTGCCTGC
  AGCCAGAAGGAGATGAGGTGGACTGGGTCCAGTGTGATGGCAGCTGCAATCAGTGGTTTCATCAGGTCTG
  TGTTGGTGTCTCTCCAGAGATGGCAGAGAAAGAAGACTACATCTGTGTGCGCTGTACTGGAAAGGACGCA
  CCAAGCCGAAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206018 representing NM_001107177
  Red=Cloning site Green=Tags(s)
  
  MEPATALPPGPRPALPLGGPGPLGEFLPPPECPVFEPSWEEFADPFAFIHKIRPIAEQTGICKVRPPPDW
  QPPFACDVDKLHFTPRIQRLNELEAQTRVKLNFLDQIAKYWELQGSTLKIPHVERKILDLFQLNKLVAEE
  GGFAVVCKDRKWTKIATKMGFAPGKAVGSHIRGHYERILNPYNLFLSGDSLRCLQKPNLTSDTKDKEYKP
  HDIPQRQSVQPSETCPPARRAKRMRAEAMNIKIEPEEATEARTHNLRRRMGCATPKRENEKEVKSTIKQE
  PTEKKDCVLESEKDKPKSRAKKTATAVDLYVCLLCGSGNDEDRLLLCDGCDDSYHTFCLLPPLHDVPKGD
  WRCPKCLAQECSKPQEAFGFEQAARDYTLRTFGEMADAFKSDYFNMPVHMVPTELVEKEFWRLVSTIEED
  VTVEYGADIASKEFGSGFPVRDGKIKISPEEEEYLDSGWNLNNMPVMEQSVLAHITADICGMKLPWLYVG
  MCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPGYAAEQLENVMKKLAPELFVSQPDLLHQLVTIMNPNT
  LMTHEVPVYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTVDWLPLGRQCVEHYRLLHRYCVFSHD
  EMICKMASKADVLDVVVASTVQKDMAIMIEDEKALRETVRKLGVIDSERMDFELLPDDERQCIKCKTTCF
  MSAISCSCKPGLLVCLHHVKELCSCPPYKYNLRYRYTLDDLYPMMNALKLRAESYNEWALNVNEALEAKI
  NKKKSLVSFKALIEESEMKKFPDNDLLRHLRLVTQDAEKCASVAQQLLNGKRQTRYRSGGGKSQSQLTVN
  ELRQFVTQLCALPCVLSQTPLLKDLLNRVEDFQQQSQRLLSEERPRASELQQLLDVSFTFDVELPQLAEM
  RIRLEQARWLEEVQQACLDSSSLSLDDMRRLIDLGVGLAPYSAVEKAMARLQELLTVSEHWDDKAKSLLR
  ARPRHSLSSLATAVKEMEEIPAYLPNGTVLKDSVQKARDWVQDVDALQAGGRVPVLETLIELVARGRSIP
  VHLNSLPRLEMLVSEVHAWKESAAKTFLLENSPYSLLEVLCPRCDIGLLGLKRKQRKLKDPLPSGKKRST
  KLESLSDLERALTESKETASAMATLGEARLREMEALQSLRFANEEKLLSPAQDVETKVCLCQKTPATPMI
  QCELCRDAFHTSCVAVSSISQSSRIWLCPHCRRSEKPPLEKILPLLASLQRIRVRLPEGDALRYMIERTV
  NWQHRAQQLLSSGNLKLVQDQAGSGLLFSRWPASAGTASAADKVSQPPGTTSFSLPEDWDNRTSYLHSPF
  STGQSCIPLHGLSPEVNELLMEAQLLQVSLPEVQELYQTLLTKPSSAQQADRSSPVRPSSEKNDCLRGKR
  DAINSLERKLKRRPEREGLPSERWDRVKKMRTPQKKKIKLSHPKDMNNFKLERERSYDLVRNAETHSLPS
  DTSYSEQEDSEDEDAICPAVSCLQPEGDEVDWVQCDGSCNQWFHQVCVGVSPEMAEKEDYICVRCTGKDA
  PSRK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107177
• ORF Size: 4632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107177.1, NP_001100647.1
• RefSeq Size: 6276 bp
• RefSeq ORF: 4635 bp
• Locus ID: 304809
• Cytogenetics: 13q13
• MW: 175.3 kDa