Pde4b (NM_017031) Rat Tagged ORF Clone

CAT#: RR205789

Pde4b (Myc-DDK-tagged ORF) - Rat phosphodiesterase 4B, cAMP specific (Pde4b), (10 ug)

Product Images

Circular map for RR205789

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4b
• Synonyms: PDE4/IVb; Pde4B1; Pde4B2; Pde4B3; Pde4B4; Pde4b5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205789 representing NM_017031
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAAAAAATTCTTCCAAAGAACTTCCAGCTTCTGAATCTGAGGTTTGCATAAAGACTTTCAAGG
  AGCAGATGCGCTTGGAACTTGAGCTTCCAAAGCTACCAGGAAACAGACCTACATCTCCCAAAATTTCTCC
  ACGCAGTTCACCAAGGAATTCACCATGCTTTTTCAGAAAGTTGCTGGTGAATAAAAGCATCCGACAGCGG
  CGTCGCTTCACTGTGGCTCATACATGCTTTGATGTGGAAAATGGCCCTTCTCCAGGTCGGAGCCCACTGG
  ACCCTCAAGCCAGCTCTTCTTCAGGACTGGTACTTCATGCCGCCTTCCCTGGGCACAGCCAACGCAGAGA
  GTCTTTTCTCTACAGATCCGACAGCGACTATGACTTGTCACCAAAAGCGATGTCAAGGAACTCCTCACTT
  CCCAGCGAACAACACGGCGATGACCTGATTGTCACTCCTTTTGCCCAGGTTCTTGCCAGCTTGCGAATCG
  TAAGAAACAATTTCACCCTGCTGACAAACCTTCACGGAGCACCGAACAAGAGGTCGCCAGCGGCTAGTCA
  GGCTCCAGTCACCAGAGTCAGCCTGCAAGAAGAATCATATCAGAAACTAGCAATGGAGACGCTGGAGGAA
  CTAGACTGGTGCCTAGACCAGCTAGAGACCATCCAGACCTACCGCTCTGTCAGCGAGATGGCTTCAAACA
  AGTTCAAAAGGATGCTGAACCGGGAGCTGACACACCTCTCAGAGATGAGCAGATCAGGGAACCAAGTGTC
  TGAATACATTTCGAACACGTTCTTAGACAAGCAGAACGATGTGGAAATCCCATCTCCCACCCAGAAGGAC
  AGGGAGAAGAAGAAGAAGCAGCAGCTCATGACCCAGATAAGTGGAGTGAAGAAACTGATGCACAGCTCAA
  GCCTGAACAACACAAGCATCTCACGCTTTGGAGTCAACACGGAAAATGAGGATCATCTAGCCAAGGAGCT
  GGAAGACCTGAACAAATGGGGCCTTAACATCTTCAACGTGGCTGGGTACTCCCATAATCGGCCCCTCACA
  TGCATCATGTACGCCATTTTCCAGGAAAGAGACCTTCTAAAGACGTTTAAAATCTCCTCCGACACCTTCG
  TAACCTACATGATGACTTTAGAAGACCATTACCATTCTGATGTGGCGTATCACAACAGCCTGCACGCTGC
  TGACGTGGCCCAGTCAACGCACGTTCTCCTCTCTACGCCAGCACTGGATGCTGTCTTCACAGACCTGGAA
  ATCCTGGCTGCCATTTTTGCAGCTGCCATCCATGATGTTGATCATCCTGGAGTCTCCAATCAGTTTCTCA
  TCAATACAAATTCCGAACTTGCTTTGATGTATAATGACGAATCTGTGCTGGAAAACCATCACCTCGCTGT
  GGGATTCAAGCTCCTTCAAGAGGAACATTGCGACATCTTTCAGAATCTTACCAAGAAGCAACGCCAGACA
  CTCAGGAAAATGGTGATTGACATGGTGTTAGCAACTGATATGTCCAAGCACATGAGCCTCCTGGCTGACC
  TTAAAACGATGGTAGAAACCAAAAAGGTGACGAGCTCCGGTGTTCTCCTCCTGGACAACTATACTGACCG
  GATACAGGTTCTTCGCAACATGGTACATTGTGCAGACCTGAGCAACCCTACCAAGTCCTTGGAGTTGTAT
  CGGCAATGGACTGATCGCATCATGGAGGAGTTTTTCCAACAGGGAGACAAAGAACGGGAGAGGGGAATGG
  AGATTAGCCCAATGTGTGATAAACACACAGCTTCTGTGGAAAAGTCCCAGGTTGGTTTCATTGACTACAT
  TGTCCATCCATTGTGGGAGACCTGGGCAGACCTGGTTCAGCCTGATGCTCAAGACATTTTGGACACACTA
  GAAGATAACAGGAACTGGTACCAGAGTATGATTCCCCAGAGCCCCTCTCCACCACTGGACGAGAGGAGCA
  GGGACTGCCAAGGCCTTATGGAGAAGTTTCAGTTCGAACTGACCCTTGAAGAAGAGGATTCTGAAGGACC
  GGAAAAGGAGGGAGAAGGCCCCAACTATTTCAGCAGCACAAAGACACTTTGTGTGATCGATCCAGAGAAC
  AGGGATTCTCTGGAAGAGACTGACATAGACATTGCCACAGAAGACAAGTCTCTGATCGACACAAGGGATT
  CTCTGGAAGAGACTGACATAGACATTGCCACAGAAGACAAGTCTCTGATCGACACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205789 representing NM_017031
  Red=Cloning site Green=Tags(s)
  
  MTAKNSSKELPASESEVCIKTFKEQMRLELELPKLPGNRPTSPKISPRSSPRNSPCFFRKLLVNKSIRQR
  RRFTVAHTCFDVENGPSPGRSPLDPQASSSSGLVLHAAFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSL
  PSEQHGDDLIVTPFAQVLASLRIVRNNFTLLTNLHGAPNKRSPAASQAPVTRVSLQEESYQKLAMETLEE
  LDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKD
  REKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLT
  CIMYAIFQERDLLKTFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLE
  ILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQT
  LRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELY
  RQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTL
  EDNRNWYQSMIPQSPSPPLDERSRDCQGLMEKFQFELTLEEEDSEGPEKEGEGPNYFSSTKTLCVIDPEN
  RDSLEETDIDIATEDKSLIDTRDSLEETDIDIATEDKSLIDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017031
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3133 bp
• RefSeq ORF: 2166 bp
• Locus ID: 24626
• UniProt ID: P14646
• Cytogenetics: 5q33
• MW: 82.1 kDa
• Gene Summary: contains four isoforms expressed from the same gene; isoform Pde4b4 has cAMP specific phosphdiesterase activity and is inhibited by rolipram [RGD, Feb 2006]