Zc3h12c (NM_001108146) Rat Tagged ORF Clone

CAT#: RR205513

Zc3h12c (Myc-DDK-tagged ORF) - Rat zinc finger CCCH type containing 12C (Zc3h12c), (10 ug)

Product Images

Circular map for RR205513

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zc3h12c
• Synonyms: RGD1565078
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205513 representing NM_001108146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGAGAAGACATTGCAGGAATACGGGGTGCTTTGCATTCAAGAATACAGGAAAAGCAGCAAAG
  TGGAGTCAAGTGCACGGAACAGCTTCATGGGCTTGAAGGATCACCTGGGGCATGACCTTGGCCACCTTTA
  TATGGAGAGCGCTGACCCACAGATAAGTGCAGCTGTGCCTTGGCCCATGGCAGAGAAACCAACAATGGAT
  ACAGTCAGTGCAGGGAAGGAAGAAAAGGGGGTGTCTGAAGAGAACGTGAGCTCTGGTGACTCTGAGGAAA
  GCACGAATTCTGATCATGAGTCAGAACAACTGAGTAGCCTTTCGGTAGAGCCATGCTTATTAACCAAGAC
  TCACAGACAATTGTGTCGGTCTCCCTGCTTAGAACCTCACCTACTGAAACACAGTGATGTTTTGCAAGAC
  TTTAAACCAGAAGAGTCCCAGACTCCATCCAAGGAAGTGAAGAAACCCCCTGATGTGGTTCGAGAATACC
  AAACAAAACTGGAGTTTGCACTTAAGTTAGGTTATTCTGAAGAACAGGTCCAGCTTGTGCTAAACAAACT
  TGGTACTGATGCTTTAATCAATGATATATTGGGAGAACTTGTCAAACTTGGAAATAAAAGTGAAGCTGAT
  CAAACAGTTAGTACTGTTAACAGTGCGATGCGAGAAACATCGTCCCTGGAGTCTCAGAGGTCTGAATCGC
  CAATGCCAGAGATCGTGACAGATGATGGTGAAAATCTGAGGCCAGTAGTTATTGATGGCAGCAATGTGGC
  AATGAGCCATGGAAACAAAGAAGTGTTCTCATGCCGAGGAATAAAATTGGCAGTGGATTGGTTTTTGGAA
  AGAGGCCACAAAGATATCACGGTCTTTGTTCCAGCTTGGAGAAAAGAACAGTCGCGACCTGACGCCCTTA
  TCACAGATCAGGAAATTTTACGTAAACTAGAGAAGGAAAAAATCCTGGTGTTCACACCATCACGGCGTGT
  CCAGGGCAGGCGCGTGGTGTGCTATGACGACAGGTTCATCGTGAAGCTGGCTTTTGAATCCGATGGTATA
  ATTGTGTCCAATGATAACTACAGAGATCTGGCAAATGAAAAGCCAGAATGGAAGAAGTTCATAGATGAAC
  GGCTATTGATGTACTCCTTCGTCAATGACAAGTTCATGCCCCCAGATGACCCACTTGGCAGACATGGCCC
  AAGTCTGGATAATTTTCTGAGGAAGAAGCCCATTGTTCCTGAACACAAAAAGCAGCCCTGTCCCTATGGA
  AAGAAGTGTACGTATGGACACAAGTGCAAATATTACCACCCAGAAAGGGGCAGCCAGCCTCAGCGCTCAG
  TGGCTGATGAACTTCGTGCCATGTCTAGAAACACAGCAGCCAAAACTACAAATGAAGGGGGACTGGTGAA
  GAGCAACAGTGTTCCTTGCAGCACCAAGGCTGATAGCACTTCGGATGTTAAACGAGGTGCTCCAAAGAGG
  CAGTCAGACCCAAGCATAAGGACTCACGTCTACCAAGACATAGAGGAAAAGCTTCCCACCAAAAACAAGT
  TGGAAACCAGGTCTGTACCTTCCTTAGTTAGTATCCCGGCCACTTCTACTGCAAAACCCCAAAGCACTAC
  ATCTTTAAGCAATGGCCTTCCATCTGGAGTTCATTTCCCACCTCAGGATCAAAGACCACAGGGACAATAC
  CCTCCAATGATGATGGCAACCAAAAATCATGGCGCGCCAATGCCTTACGAACAGTATCCAAAATGTGAGT
  CCCCTGTTGATGTTGGCTATTATTCCATGTTGAACGCATATTCTAATCTGAGTATCTCAGGCCCCCGAAG
  TCCTGAAAGGCGTTTCTCCCTAGACACTGATTATAGAGTCAATTCTGTAGCTTCAGACTGCAGCAGTGAG
  GGGAGCATGAGCTGTGGGAGCAGCGACTCCTACGTGGGGTACAGTGACCGCTCCTACGTCAGCTCTCCTG
  ACCCACAGCTGGAAGAGAATCTCAAGGGTCAACACATGCACCCTCACAGCCGCCTTAACTCCCAGCCCTT
  CCTGCAGAGTTTCCACGATCCCTTAACCAGAGTTCAGAGTTACAGTCACGAAGAACCAAAGTTCCATCAC
  AAGCGTCCTCTTCCACACTTGGCTATGCATCTGCAGCACCCTGCCGTGGGTGCCCGCTCCAGCTGTCCTG
  GCGACTACCCCTCTCCACCAAGTTCAGCACACTCTAAGGCATCACACCTAGGGAGGTCCTTAGTGGCCAC
  GAGAATAGACAGCATTTCTGACTCTCGACTCTATGATAGTTCTCCTTCAAGACAAAGAAAGCCTTATTCT
  CGCCAGGAAGGCCTAGGAAGCTGGGATAGGCCAAGCTATGGGATGGACGCGTATGGCTACCGGCAGACTT
  ACTCCTTACCCGACAATTCCACACCCCCCTGCTATGAGTCGATCACCTTCCAGAGCCTGCCCGAGCAGCA
  GGAGCCGACCTGGAGGATCCCTTACTGCGGAATGCCACACGACCCTCCGAGGTACCAGGACAGCCGAGAG
  AAGATTTATATCAACTTGTGCAACATCTTCCCCCCTGACCTTGTGAGGCTCGTCATGAAGAGGAATCCTC
  ACATGACAGATGCCCAGCAGCTCGCGGCAGCCATCTTAGTGGAGAAGTCGCAGCTGGGTTAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205513 representing NM_001108146
  Red=Cloning site Green=Tags(s)
  
  MAAEKTLQEYGVLCIQEYRKSSKVESSARNSFMGLKDHLGHDLGHLYMESADPQISAAVPWPMAEKPTMD
  TVSAGKEEKGVSEENVSSGDSEESTNSDHESEQLSSLSVEPCLLTKTHRQLCRSPCLEPHLLKHSDVLQD
  FKPEESQTPSKEVKKPPDVVREYQTKLEFALKLGYSEEQVQLVLNKLGTDALINDILGELVKLGNKSEAD
  QTVSTVNSAMRETSSLESQRSESPMPEIVTDDGENLRPVVIDGSNVAMSHGNKEVFSCRGIKLAVDWFLE
  RGHKDITVFVPAWRKEQSRPDALITDQEILRKLEKEKILVFTPSRRVQGRRVVCYDDRFIVKLAFESDGI
  IVSNDNYRDLANEKPEWKKFIDERLLMYSFVNDKFMPPDDPLGRHGPSLDNFLRKKPIVPEHKKQPCPYG
  KKCTYGHKCKYYHPERGSQPQRSVADELRAMSRNTAAKTTNEGGLVKSNSVPCSTKADSTSDVKRGAPKR
  QSDPSIRTHVYQDIEEKLPTKNKLETRSVPSLVSIPATSTAKPQSTTSLSNGLPSGVHFPPQDQRPQGQY
  PPMMMATKNHGAPMPYEQYPKCESPVDVGYYSMLNAYSNLSISGPRSPERRFSLDTDYRVNSVASDCSSE
  GSMSCGSSDSYVGYSDRSYVSSPDPQLEENLKGQHMHPHSRLNSQPFLQSFHDPLTRVQSYSHEEPKFHH
  KRPLPHLAMHLQHPAVGARSSCPGDYPSPPSSAHSKASHLGRSLVATRIDSISDSRLYDSSPSRQRKPYS
  RQEGLGSWDRPSYGMDAYGYRQTYSLPDNSTPPCYESITFQSLPEQQEPTWRIPYCGMPHDPPRYQDSRE
  KIYINLCNIFPPDLVRLVMKRNPHMTDAQQLAAAILVEKSQLGY
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108146
• ORF Size: 2652 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108146.1, NP_001101616.1
• RefSeq Size: 2709 bp
• RefSeq ORF: 2655 bp
• Locus ID: 315658
• Cytogenetics: 8q24
• MW: 99.4 kDa