Axin1 (NM_024405) Rat Tagged ORF Clone

CAT#: RR205458

Axin1 (Myc-DDK-tagged ORF) - Rat axin 1 (Axin1), (10 ug)

Product Images

Circular map for RR205458

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Axin1
• Synonyms: Axin; axin-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205458 representing NM_024405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAGTCCCAAAATGAATGTCCAGGAGCAGGGTTTCCCCTTGGACCTCGGAGCAAGTTTCACCGAAG
  ATGCCCCCCGGCCCCCAGTGCCTGGAGAAGAGGGAGAACTGGTATCCACTGATTCGAGGCCTGTCAACCA
  CAGTTTCTGCTCTGGGAAAGGTACCAGCATTAAAAGTGAGACCTCAACAGCCACCCCAAGACGTTCAGAT
  CTGGATCTGGGATATGAGCCTGAGGGCAGTGCCTCCCCCACCCCACCATACTTGAGGTGGGCTGAGTCAC
  TGCATTCCTTACTGGATGACCAAGATGGGATCAGCCTGTTCAGGACTTTCCTGAAGCAGGAGGGCTGTGC
  TGACCTGCTGGACTTCTGGTTTGCCTGCAGTGGCTTCAGAAAGCTTGAGCCTTGTGACTCAAATGAGGAA
  AAGAGGCTGAAGCTGGCAAGAGCCATCTACCGAAAGTACATCCTGGATAGCAATGGCATTGTGTCCAGAC
  AAACCAAGCCAGCCACTAAGAGCTTCATAAAGGACTGCGTCATGAAGCAGCAGATCGATCCTGCCATGTT
  TGACCAGGCACAGACAGAAATCCAGTCCACCATGGAGGAGAACACCTACCCTTCCTTCCTTAAGTCTGAC
  ATTTATTTGGAGTACACAAGGACAGGCTCAGAAAGTCCAAAGGTCTGCAGTGACCAGAGCTCAGGGTCCG
  GGACAGGGAAGGGCATGTCTGGATACTTGCCCACTTTGAATGAAGATGAAGAATGGAAATGTGACCAAGA
  TGCAGATGAGGATGATGGCCGAGACTCTGTCCCCCCCAGCAGGCTCACCCAGAAGCTGCTATTGGAGACT
  GCTGCCCCGAGGGCCCCCTCAAGTAGACGGTACAACGAAGGCAGAGAGCTCAGGTATGGATCTTGGAGGG
  AGCCTGTCAACCCCTACTATGTCAACTCTGGCTATGCCCTGGCCCCAGCCACCAGTGCCAATGACAGTGA
  GCAGCAGAGCCTGTCCAGTGATGCTGACACTCTATCCCTCACGGACAGTAGCGTGGATGGAATCCCCCCA
  TACAGGATCCGTAAGCAGCACCGAAGGGAGATGCAGGAGAGTGTCCAAGTCAATGGGCGGGTACCTCTAC
  CTCACATTCCTCGCACTTACCGAATGCCAAAGGAGATCCGGGTAGAGCCACAGAAATTTGCTGAAGAGCT
  TATTCACCGTCTAGAGGCTGTCCAGCGTACTCGAGAGGCTGAAGAAAAGTTGGAGGAACGGCTGAAGCGT
  GTACGCATGGAGGAAGAAGGGGAAGATGGGGAAATGCCTTCTGGCCCCATGGCAAGTCACAAGCTGCCTT
  CTGTCCCAGCTTGGCACCATTTCCCACCTCGCTATGTGGATATGGGCTGCTCCGGACTGCGGGATGCCCA
  TGAGGAGAATCCTGAGAGCATCCTGGATGAGCATGTGCAAAGGGTCATGAGGACACCTGGCTGCCAGTCA
  CCTGGCCCAGGCCACCGCTCTCCTGACAGCGGGCATGTGGCCAAGACTGCAGTGCTAGGGGGTACAGCAT
  CTGGGCATGGGAAGCATGCTCCTAAGTTAGGGTTGAAGCTGGATTCAGCTGGCCTGCACCACCATAGGCA
  CGTCCACCACCACGTTCACCATAATTCAGCTAGACCCAAGGAGCAAATGGAGGCTGAAGCTGCCCGCAGG
  GTCCAGAGCAGCTTCTCATGGGGCCCAGAAACACATGGTCATGCCAAGCCCCGAAGCTATTCAGAGAGCA
  CAGGCACCAACCCCAGTGCTGGGGACTTGGCCTTTGGTGGTAAAGCTAGTGCACCCTCCAAAAGAAACAC
  CAAGAAGGCTGAATCTGGGAAGAATGCCAGTGCTGAGGTACCCAGTACCACAGAGGATGCAGAGAAGAAC
  CAGAAGATCATGCAGTGGATCATTGAGGGGGAGAAGGAGATCAGTAGACACCGGAAGGCAGGCCATGGGT
  CTTCTGGGATGAGGAAGCAGCAGGCCCATGAAAGCTCCAGGCCCTTGTCCATTGAACGTCCTGGGGCCGT
  GCACCCCTGGGTCAGTGCTCAGCTTCGGAACTCTGTCCAGCCTTCTCATCTCTTCATCCAAGATCCCACA
  ATGCCACCCAATCCAGCCCCTAATCCTCTGACCCAGCTGGAAGAGGCCCGCAGGCGTTTGGAGGAAGAAG
  AAAAGAGAGCAAACAAACTGCCCTCCAAGCAGAGGACAAAATCACAAAGAAAGGCAGGTGGCGGGAGTGC
  ACCACCATGTGACAGTATCGTTGTGGCCTACTATTTCTGTGGTGAACCAATACCCTACCGGACCCTGGTG
  AGGGGCCGTGCTGTCACCCTGGGCCAGTTCAAGGAGCTGCTAACCAAGAAGGGGAGCTACAGATACTACT
  TTAAGAAAGTGAGTGACGAGTTTGACTGTGGTGTGGTATTTGAGGAAGTACGGGAAGATGAGGCCATCCT
  GCCTGTCTTTGAAGAAAAGATCATCGGCAAGGTGGAAAAGGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205458 representing NM_024405
  Red=Cloning site Green=Tags(s)
  
  MQSPKMNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSD
  LDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEE
  KRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSD
  IYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDSVPPSRLTQKLLLET
  AAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPP
  YRIRKQHRREMQESVQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKR
  VRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQS
  PGPGHRSPDSGHVAKTAVLGGTASGHGKHAPKLGLKLDSAGLHHHRHVHHHVHHNSARPKEQMEAEAARR
  VQSSFSWGPETHGHAKPRSYSESTGTNPSAGDLAFGGKASAPSKRNTKKAESGKNASAEVPSTTEDAEKN
  QKIMQWIIEGEKEISRHRKAGHGSSGMRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPT
  MPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLV
  RGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAILPVFEEKIIGKVEKVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024405
• ORF Size: 2496 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024405.1, NP_077381.1
• RefSeq Size: 3460 bp
• RefSeq ORF: 2499 bp
• Locus ID: 79257
• UniProt ID: O70239
• Cytogenetics: 10q12
• MW: 92.9 kDa
• Gene Summary: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (By similarity). Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway (By similarity). Also facilitates the phosphorylation of APC by GSK3B (By similarity). Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation (By similarity). Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 (By similarity).[UniProtKB/Swiss-Prot Function]