Panx2 (NM_199409) Rat Tagged ORF Clone

CAT#: RR205173

Panx2 (Myc-DDK-tagged ORF) - Rat pannexin 2 (Panx2), (10 ug)

Product Images

Circular map for RR205173

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Panx2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205173 representing NM_199409
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACCACCTCCTGGAGCAGTCGGCGGACATGGCGACCGCGCTGCTGGCGGGCGAGAAGCTGAGGGAGC
  TGATCCTGCCTGGCTCGCAGGACGACAAGGCGGGTGCGCTGGCCGCGCTGCTGCTGCAGCTCAAGCTGGA
  ACTGCCGTTCGACCGCGTGGTCACCATCGGCACCGTGCTGGTACCCATCCTGCTGGTCACCCTGGTCTTC
  ACCAAGAACTTCGCAGAGGAACCAATTTACTGTTATACTCCGCACAACTTCACCCGTGACCAGGCGCTGT
  ACGCCCGTGGCTACTGCTGGACAGAGCTGCGGGATGCCCTGCCCGGCGTGGATGCCAGCCTGTGGCCATC
  GCTGTTTGAGCACAAGTTCCTGCCCTACGCACTGCTGGCCTTTGCCGCCATCATGTATGTGCCCGCACTG
  GGCTGGGAGTTCCTGGCCTCCACGCGCCTCACCTCCGAGCTCAACTTCCTGCTTCAGGAGATCGACAACT
  GCTACCACCGAGCCGCCGAAGGTCGCGCCCCCAAGATTGAGAAGCAGATCCAGTCCAAGGGGCCAGGCAT
  CACGGAGCGCGAGAAGCGGGAAATCATTGAGAACGCTGAGAAGGAGAAGAGCCCTGAGCAGAATTTGTTT
  GAGAAGTACCTGGAGCGCCGGGGCCGCAGCAACTTCTTGGCCAAGCTGTACTTGGCGCGGCACGTACTGA
  TCCTGCTACTCAGTGTGGTGCCCATCTCTTACCTATGCACATACTACGCCACCCAGAAGCAGAACGAGTT
  CACCTGCGCCCTGGGCGCCTCGCCTGACGGGCCGGTGGGTAGCGCCGGGCCCACGGTGCGCGTCAGCTGT
  AAGCTGCCCTCCGTGCAGCTGCAGCGGATCATTGCGGGTGTGGACATCGTATTGCTCTGCTTCATGAACC
  TCATCATCCTGGTCAACCTCATCCACCTCTTCATCTTCCGCAAGAGCAACTTCATTTTTGACAAGCTGCA
  CAAGGTGGGTATCAAGACGCGCCGGCAGTGGCGCCGCTCGCAGTTCTGCGACATCAACATCCTGGCCATG
  TTCTGTAACGAGAACCGTGACCACATCAAGTCGCTTAACCGGCTGGACTTCATCACCAACGAGAGCGACC
  TCATGTACGACAACGTGGTGCGGCAGCTTCTGGCTGCCTTGGCTCAGTCTAACCACGACACCACGCCCAC
  GGTTCGGGACTCAGGCATCCAGACCGTGGACCCCAGCATCAACCCCGCAGAGCCTGAGGGCTCTGCTGAG
  CCACCCGTGGTCAAGCGGCCCCGTAAGAAAATGAAGTGGATCCCCACCAGCAACCCGCTGCCACAGCCCT
  TCAAGGAGCAGCTGGCCATTATGCGCGTAGAAAACAGCAAGACTGAGAAGCCCAAACCTGTGCGCAGGAA
  GACAGCCACGGATACACTTATTGCCCCGCTGCTGGACGCTGGTGCCCGGGCTGCCCACCACTACAAGGGG
  AGTGGAGGTGATACAGGGCCCTCCTCTGCCCCGCCTGCTGCCTCTGAGAAAAAGCATACCCGGCACTTCT
  CCTTGGATGTGCATCCCTATATCCTAGGCAGCAAGAAGGCCAAGACTGAAGCAGTACCCCCTGCCCTACC
  AGCCTCCCGGAGCCAGGAAGGTGGCTTCCTGTCCCAGACAGAGGAATGTGGGCTGGGCTTGGCTGCAGCA
  CCCACCAAAGATGCTCCGCTCCCTGAGAAGGAAATCCCATACCCCACAGAGTCTGCCCTGCCGTCTGGGG
  GCCCATTCCATGTGTGCTCACCCCCCACGGCCTCTGCTGCTGCTTCCCTGTCACCGAGCAGCCTGGGCAA
  GGCTGACCCCCTCACCATCCTGAGCCGAAACGCCACTCACCCCCTGCTCCACATCAGCACGTTGTACGAG
  GCCCGGGAAGAGGAGGAAGGAGGTCCCTGTGCACCCTCAGACATGGGCGACCTCCTCAGCATACCCCCAC
  CCCAGCAGATCCTCATCGCCACCTTCGAGGAGCCGAGAACAGTTGTGAGTACTGTGGAGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205173 representing NM_199409
  Red=Cloning site Green=Tags(s)
  
  MHHLLEQSADMATALLAGEKLRELILPGSQDDKAGALAALLLQLKLELPFDRVVTIGTVLVPILLVTLVF
  TKNFAEEPIYCYTPHNFTRDQALYARGYCWTELRDALPGVDASLWPSLFEHKFLPYALLAFAAIMYVPAL
  GWEFLASTRLTSELNFLLQEIDNCYHRAAEGRAPKIEKQIQSKGPGITEREKREIIENAEKEKSPEQNLF
  EKYLERRGRSNFLAKLYLARHVLILLLSVVPISYLCTYYATQKQNEFTCALGASPDGPVGSAGPTVRVSC
  KLPSVQLQRIIAGVDIVLLCFMNLIILVNLIHLFIFRKSNFIFDKLHKVGIKTRRQWRRSQFCDINILAM
  FCNENRDHIKSLNRLDFITNESDLMYDNVVRQLLAALAQSNHDTTPTVRDSGIQTVDPSINPAEPEGSAE
  PPVVKRPRKKMKWIPTSNPLPQPFKEQLAIMRVENSKTEKPKPVRRKTATDTLIAPLLDAGARAAHHYKG
  SGGDTGPSSAPPAASEKKHTRHFSLDVHPYILGSKKAKTEAVPPALPASRSQEGGFLSQTEECGLGLAAA
  PTKDAPLPEKEIPYPTESALPSGGPFHVCSPPTASAAASLSPSSLGKADPLTILSRNATHPLLHISTLYE
  AREEEEGGPCAPSDMGDLLSIPPPQQILIATFEEPRTVVSTVEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199409
• ORF Size: 2022 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199409.2, NP_955441.2
• RefSeq Size: 3376 bp
• RefSeq ORF: 2025 bp
• Locus ID: 362979
• UniProt ID: P60571
• Cytogenetics: 7q34
• MW: 74.4 kDa
• Gene Summary: structural component of gap junctions; forms heteromeric intercellular channels with pannexin 1; may facilitate electrical communication in the brain [RGD, Feb 2006]