Hes1 (NM_024360) Rat Tagged ORF Clone

CAT#: RR204727

Hes1 (Myc-DDK-tagged ORF) - Rat hairy and enhancer of split 1 (Drosophila) (Hes1), (10 ug)

Product Images

Circular map for RR204727

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hes1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204727 representing NM_024360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCTGATATAATGGAGAAAAATTCCTCGTCCCCGGTGGCTGCTACCCCAGCCAGTGTCAACACGA
  CACCGGACAAACCAAAGACAGCCTCTGAGCACAGAAAGTCATCAAAGCCTATCATGGAGAAGAGGCGCCG
  GGCAAGAATAAATGAAAGTTTGAGCCAACTGAAAACACTGATTTTGGATGCACTTAAGAAAGATAGCTCC
  CGGCATTCCAAGCTGGAGAAGGCAGACATTCTGGAAATGACAGTGAAGCACCTCCGGAACCTGCAGCGGG
  CGCAGATGACCGCCGCTCTCAGCACAGACCCGAGCGTGTTGGGGAAGTACCGCGCCGGCTTCAGCGAGTG
  CATGAACGAGGTGACCCGCTTCCTGTCCACGTGTGAGGGCGTTAACACCGAGGTGCGCACTCGGCTGCTG
  GGCCACCTGGCCAACTGCATGACCCAGATCAACGCCATGACCTACCCCGGGCAGGCGCACCCCGCCTTGC
  AGGCGCCGCCGCCGCCGCCCCCGTCAGGACCTGGCGGTCCCCAGCACGCGCCATTCGCGCCGCCGCCGCC
  GCTTGTGCCCATCCCCGGGGGCGCGGCGCCCCCTCCCGGCAGCGCACCCTGCAAGTTGGGCAGCCAGGCT
  GGAGAGGCTGCCAAGGTTTTTGGCGGCTTCCAAGTGGTGCCGGCTCCTGACGGCCAATTTGCTTTCCTCA
  TCCCCAATGGGGCCTTCGCCCACAGCGGCCCGGTCATCCCGGTCTACACCAGCAACAGCGGGACCTCGGT
  GGGTCCTAACGCAGTGTCGCCTTCCAGCGGCTCCTCGCTCACTGCGGACTCCATGTGGAGGCCGTGGCGG
  AAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204727 representing NM_024360
  Red=Cloning site Green=Tags(s)
  
  MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSS
  RHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLL
  GHLANCMTQINAMTYPGQAHPALQAPPPPPPSGPGGPQHAPFAPPPPLVPIPGGAAPPPGSAPCKLGSQA
  GEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGSSLTADSMWRPWR
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024360
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024360.3, NP_077336.3
• RefSeq Size: 1436 bp
• RefSeq ORF: 846 bp
• Locus ID: 29577
• UniProt ID: Q04666
• Cytogenetics: 11q22
• MW: 29.6 kDa
• Gene Summary: may act as a transcriptional repressor; may inhibit cell adhesion molecule NCAM-dependent neurite outgrowth [RGD, Feb 2006]