Akap1 (NM_053665) Rat Tagged ORF Clone

CAT#: RR204698

Akap1 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 1 (Akap1), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR204698

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akap1
• Synonyms: Akap84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR204698 representing NM_053665
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATCCAGTTCCGCTCACTCTTCCCCTTGGCCTTGCCTGGAATGCTAGCACTCCTTGGCTGGTGGT
  GGTTTTTCTCTCGTAAAAAAGATCGGCTCAGCAGCAATGGCAAGCAGGTGGGGACATTGAAGGTTGGCCC
  TGCCATCGAGGACCGACTGCCCACTGAAGAGGCCTGTCCTGGAGTCCTGTCTGTGACCCCCAGTGTCACA
  CAGCCTCCTGGAAAGGAAGAGCAGCGCTCTATGGACAGGCCTCTTTCAGATCCCCCAGCCTTGCCAAGGA
  CCCGTCAAGTTCGCCGAAGATCAGAGTCCTCAGGCAACCTCCCCAGCATTGTAGACACAAGGTTGCAGGC
  AGGACAGTGCAGCGATGAGAATTCAAAAGTGGTACTCTCCCTGATGGGGGACGAAGCCAAATCTATTCCT
  CTTGGACGTCCGCTTTTCCCAAAGGATCTGTCCTTCCCCTATGAAGCAGTGGAAGGGTGTAAGCAAGAGT
  CTGCCCTGGGCAGAACTCCTGGGAGAGGCTGGCTAAGCCAGTGTGCAGCCTCTGGAGAGAACGCGAGAGA
  GACGGGTGGGGCGGAAGGGACTGGAGATGCTGTGTTGGGGGAAAGTGTACTTGAGGAAGGCCTATTGCCC
  CAGGAGTGTGTTTCAGAAGTAGAGAAGAGTGAGTTTCCGATCCTAGCTCCCGGGGGAGGCGGGGGAGAGA
  AGGTGAGAAGTGGCCCACCACAGGTAGATGAGCTTTTAAAGAAGGAAGAATATATTGTTGGGAAGTTGCC
  AAGTAGCTTTGTGGGGCCAGTTCACTCGGAGCTGGTTAAGGATGAGGGTGCGCTGGTACCCCAAGTCAAA
  GGTAGCCAGGACAGAAGCCTGGCTAGAGAACTGGACAAAGACAAGACCTTGCCTGAAAAGGACCAGATTG
  AGCAGACTGCCTTCCAGATCATCTCCCAGGTGATCTTGGAAGCGACTGAAGAGATACGGGCCACCACAGT
  GGGCAAGACTGTGGCACAAGTGCATCCAACCCCGGGCACTCAGCCTCAGGGGCAGGAGGAGAGCTGTGTC
  CCAGCCAGCCAGGAAACTAGCTTGGGACAAGAAATCCCAGATCCTGCTTCCACCAGAACAGGTGCCACTG
  CCAGCCCTTCAGCAGGAGCCCCACCACCAAAGACCTATGTGAGCTGTCTCAGCAGTCCTCTGTCAGGCCC
  CACCAAGGACCAGAAGCCAAAGAACTCTGCACACCACATCTCCCTGGCTCCCTGCCCACCGCCAGTTACC
  CCCCAGAGGCAGTCTCTGGATGGGGCAAGTAACCCGAGAGGTGATGACACCTTTGTCACCTGTACGTCCA
  ACAACAGCCAGAGTGTCCTTTCAGTTACCTCCTTGGGGCTGTGCTCAGACCCTGTCAGTACTTCGAGGCT
  TGAAGACTCTTGCACAGAGACCATCTCAAGCTCCGGAGACAAAGCTGTCACCCCACCACTGCCAGACAGT
  ACTGAGCCCTTCAGCAATGGGGTGCTGAAGGAGGAGTTGTCAGACTTGGGGACCGAGGATGGATGGACCA
  TGGATACAGAAGCAGATCATTCAGGAGGTTCTGACGGGAACAGTATGGATTCAGTGGATAGCTGTTGTGG
  GCTTACGAAACCTGATAGCCCCCAGACTGTCCAGGCAGGCTCCAACCCTAAGAAGGTTGACCTCATCATC
  TGGGAGATCGAGGTGCCAAAGCATTTAGTTGGTCGACTGATTGGCAAGCAGGGACGGTACGTGAGTTTTC
  TGAAGCAGACGTCTGGTGCCAAGATCTACATCTCCACCCTGCCTTACACACAGAACATCCAGATCTGCCA
  CATAGAAGGCTCTCAGCATCATGTGGACAAAGCTCTGAACTTGATTGGGAAGAAGTTTAAGGAGCTGAAC
  CTCACCAATATCTATGCACCACCGCTGCCTTCTCTGGCACTGCCTTCCCTGCCGATGACATCGTGGCTCA
  TGCTGCCTGATGGTATCACTGTGGAAGTCATCGTGGTCAACCAGGTCAATGCTGGGCACCTATTTGTACA
  GCAACACACACACCCCACCTTCCACGCACTGCGCAGCCTGGACCAGCAGATGTACCTCTGTTACTCTCAG
  CCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTTATCTGCGCTGCCCCTGGTGCGGACGGGG
  CCTGGTGGCGAGCCCAAGTAGTGGCCTCCTATGAGGAGACCAATGAGGTGGAGATTCGCTACGTGGACTA
  TGGTGGATATAAGAGGGTGAAAGTCGACGTGCTCCGGCAAATTAGGTCTGACTTTGTGACCCTGCCATTC
  CAAGGAGCAGAAGTCCTTCTGGACAGTGTGGTTCCGCTGTCAGATGATGATCACTTTTCACCCGAGGCAG
  ACGCAGCCATGAGTGAGATGACAGGCAATACAGCACTGCTGGCCCAGGTGACAAGCTACAGTGCGACTGG
  CCTTCCTCTGATTCAGCTATGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCGCTGGTGGAG
  CGGGGCCTGGCACAGTGGGTAGACAGCTGCTATGCCAGCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR204698 representing NM_053665
  Red=Cloning site Green=Tags(s)
  
  MAIQFRSLFPLALPGMLALLGWWWFFSRKKDRLSSNGKQVGTLKVGPAIEDRLPTEEACPGVLSVTPSVT
  QPPGKEEQRSMDRPLSDPPALPRTRQVRRRSESSGNLPSIVDTRLQAGQCSDENSKVVLSLMGDEAKSIP
  LGRPLFPKDLSFPYEAVEGCKQESALGRTPGRGWLSQCAASGENARETGGAEGTGDAVLGESVLEEGLLP
  QECVSEVEKSEFPILAPGGGGGEKVRSGPPQVDELLKKEEYIVGKLPSSFVGPVHSELVKDEGALVPQVK
  GSQDRSLARELDKDKTLPEKDQIEQTAFQIISQVILEATEEIRATTVGKTVAQVHPTPGTQPQGQEESCV
  PASQETSLGQEIPDPASTRTGATASPSAGAPPPKTYVSCLSSPLSGPTKDQKPKNSAHHISLAPCPPPVT
  PQRQSLDGASNPRGDDTFVTCTSNNSQSVLSVTSLGLCSDPVSTSRLEDSCTETISSSGDKAVTPPLPDS
  TEPFSNGVLKEELSDLGTEDGWTMDTEADHSGGSDGNSMDSVDSCCGLTKPDSPQTVQAGSNPKKVDLII
  WEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQNIQICHIEGSQHHVDKALNLIGKKFKELN
  LTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQ
  PGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPF
  QGAEVLLDSVVPLSDDDHFSPEADAAMSEMTGNTALLAQVTSYSATGLPLIQLWSVVGDEVVLINRSLVE
  RGLAQWVDSCYASL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053665
• ORF Size: 2562 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053665.1, NP_446117.1
• RefSeq Size: 2621 bp
• RefSeq ORF: 2565 bp
• Locus ID: 114124
• UniProt ID: O88884
• Cytogenetics: 10q26
• MW: 91.7 kDa
• Gene Summary: scaffolding protein that immobilizes and concentrates protein kinase A II (PKAII) isoforms at specific intracellular locations [RGD, Feb 2006]