Adam28 (NM_181693) Rat Tagged ORF Clone

CAT#: RR203914

Adam28 (Myc-DDK-tagged ORF) - Rat ADAM metallopeptidase domain 28 (Adam28), (10 ug)

Product Images

Circular map for RR203914

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203914 representing NM_181693
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCAATGGCGACTTCTGGTAGTCTGGTTCCTCCTTTCTCCAGTTCCAGCAAGTGCAATAAAAGAAC
  TCCCTAAAGCCAAGAATTATGAAGTGGTTTATCCCATAAGACTTCATCTGTTGCATAAAAGAGAGACCAA
  AGAGCCAGAGCCAAAGGAAACATTTGAAACTGAGCTCAGGTACAAAATGACAGTAAACGGAAAGGTTGTG
  GAGCTATACCTGAAGAAGAACAACAAGCTCCTAGCACCTGGCTACTTGGAAACATACTATAATTCCAGTG
  GAAACAAGGTCACCACAAGCCCGCAAATCATGGATAGCTGTTACTACCAAGGACACATCATAAACGAGAA
  AGATTCAGCAGCCAGCATCAGCATGTGTCAAGGGCTACGGGGTTACTTCAGTCAAGCTGATGAAAGGTAT
  TTTATTGAACCTTTGAGCTCGGAGATCTTGGATGAGCAGGCACATGCACTCTTCAAGGATGATCCCAAAG
  AAGACCAGGGGAACAGTAACTGTGGAGTGGATGATGCGCTATGGCTCCAAGGACTGCATCAGGATGTGGT
  CCTTCCTGCCACCAGGTTGATTAAGCTGAATGATGGGATGGTCCAAGAACCTAAGAAGTACATAGAATAT
  TATGTGGTCCTGGACAATGGTGAGTTTAAGAAATACAATAAAAATCTTGATGAAATAAGAAAGAGAGTAC
  ATGAGATGGCCAATTATGTCAACATGCTTTACAATAAGCTTGGTGCCCATGTGGCCTTAGTTGGAATGGA
  AATCTGGACTGATGAGGATAAAATAAAGATAACACCAGATGCCAACACCACCCTGGAAAACTTCTCTAAG
  TGGAGGGGAAATGATCTGCTAAAGCGAAAGCACCATGATGTTGCCCAGCTAATCTCCTCAACAGATTTTT
  CTGGTTCAACAGTTGGTCTAGCCTTCATGTCTTCAATGTGTTCTCCTTACCATTCTGTTGGCATTGTTCA
  GGACCACAGTAACTACCATCTTCGAGTCGCAGGAACAATGGCACATGAAATGGGTCACAACCTTGGCATG
  ATTCATGACTACTTGAGTTGTAAGTGCCCATCTGAAGTCTGTGTAATGGAGCAGTCACTAAGATTCCACA
  TGCCTACAGACTTCAGCTCCTGCAGTCGTGACAATTACAGACGATTTCTTGAAGAGAAATTATCTCATTG
  CCTCTTTAATAGTCCTTTGCCTTCAGATATCATATCCACCCCAGTCTGTGGGAACCAGTTATTGGAAATG
  AATGAGGACTGTGACTGTGGCACTCCCAAGGAGTGTACCAACAAATGCTGTGATGCAGAGACCTGTAAAA
  TTAAAGCAGGTTTCCAGTGTGCTTTGGGAGAATGCTGCGAGAAATGCCAACTTAAAAAGCCTGGGGTTGT
  GTGCAGAGCAGCAAAAGATGAGTGTGATCTGCCTGAAATGTGTGATGGTAAATCCAGCCACTGCCCAGTT
  GACAGATTCAGAGTCAATGGCTTCCCTTGCCAAAATGGGCATGGCTACTGCTTGAAGGGCAACTGTCCCA
  CCCTGCAGCAGCAGTGCATGGACATGTGGGGCCCAGAAACCAAGGTTGCAAATAAGTCATGTTACAAGCA
  GAATGAAGGTGGGTCAAAGTACGGATACTGTCACGTGGAGAATGGTACCCACATGCCCTGCAAAGCCAAA
  GATGCCATGTGCGGGAAGTTGTTCTGTGAGGGTGGGTCAGGTGATCTGCCCTGGAAAGGACTTACAATAG
  CTTTCCTGACATGTAAATTATTTGATCCTGAAGACATCAATCAAGGAGTAGACATGGTGGCCAATGGAAC
  CAAATGTGGAAATAACAAGGTGTGCATTAACGCAGAGTGTGCGGACATGGAGAAGACATACAAGTCAGCC
  AACTGCTCCTCCAAGTGCAAAGGCCACGCAGTGTGTGACCATGAGCTTCAGTGTCAGTGCAAGGAAGGAT
  GGGCCCCTCCTGACTGTGAGGATTCTGCCACAGTCTTCCGTAGGCGTCTCCATCATGGTTGGCGTGCTCT
  TTCCTCTGGCAGCCATATTTGTGGTGATTGCTATAGTGATCCATCATCAAAGTGCCAGAAGAAAGCAAAG
  GAGAGTTCAGAGGCTACCACCCATCAAGGATGCCAAGCTACACAAGCAGAAGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203914 representing NM_181693
  Red=Cloning site Green=Tags(s)
  
  MQQWRLLVVWFLLSPVPASAIKELPKAKNYEVVYPIRLHLLHKRETKEPEPKETFETELRYKMTVNGKVV
  ELYLKKNNKLLAPGYLETYYNSSGNKVTTSPQIMDSCYYQGHIINEKDSAASISMCQGLRGYFSQADERY
  FIEPLSSEILDEQAHALFKDDPKEDQGNSNCGVDDALWLQGLHQDVVLPATRLIKLNDGMVQEPKKYIEY
  YVVLDNGEFKKYNKNLDEIRKRVHEMANYVNMLYNKLGAHVALVGMEIWTDEDKIKITPDANTTLENFSK
  WRGNDLLKRKHHDVAQLISSTDFSGSTVGLAFMSSMCSPYHSVGIVQDHSNYHLRVAGTMAHEMGHNLGM
  IHDYLSCKCPSEVCVMEQSLRFHMPTDFSSCSRDNYRRFLEEKLSHCLFNSPLPSDIISTPVCGNQLLEM
  NEDCDCGTPKECTNKCCDAETCKIKAGFQCALGECCEKCQLKKPGVVCRAAKDECDLPEMCDGKSSHCPV
  DRFRVNGFPCQNGHGYCLKGNCPTLQQQCMDMWGPETKVANKSCYKQNEGGSKYGYCHVENGTHMPCKAK
  DAMCGKLFCEGGSGDLPWKGLTIAFLTCKLFDPEDINQGVDMVANGTKCGNNKVCINAECADMEKTYKSA
  NCSSKCKGHAVCDHELQCQCKEGWAPPDCEDSATVFRRRLHHGWRALSSGSHICGDCYSDPSSKCQKKAK
  ESSEATTHQGCQATQAEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181693
• ORF Size: 2154 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181693.1, NP_859044.1
• RefSeq Size: 2335 bp
• RefSeq ORF: 2157 bp
• Locus ID: 290344
• Cytogenetics: 15p11
• MW: 80.6 kDa
• Gene Summary: mouse homolog is a metalloprotease disintegrin; may have a role in sperm maturation [RGD, Feb 2006]