Itga4 (NM_001107737) Rat Tagged ORF Clone

CAT#: RR203360

Itga4 (Myc-DDK-tagged ORF) - Rat integrin alpha 4 (Itga4), (10 ug)

Product Images

Circular map for RR203360

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203360 representing NM_001107737
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGAAGCGATGTGCAGACCGAGGCCCCGAGGGGTCGCCCTCCAGGAAGCGGTGATGCTGTTGC
  TGTACTTCGGGGTACACTTCAGGGTACCAACCGGGCACTCCTACAACCTGGACCCGGAGAGTGCACTGCT
  GTACCAGGGCCCCTCCGGCACGCTGTTTGGCTACTCGGTGGTGCTGCACAGCCACGGGACGAAGCGCTGG
  CTCATTGTGGGGGCGCCCACTGCCAGCTGGCTCGCCAATGCCTCGGTGGTCAATCCTGGGGCGATTTACA
  GATGCAGGATCGGAAAGAATCCAAACCAGATCTGCGAACAGCTCCAGCTGGGAAGCCCCAGTGGAGAACC
  CTGTGGGAAGACGTGCTTGGAAGAGAGAGATAATCAGTGGCTGGGAGTGACAATTTCCAGACAGCCAGGA
  GAAAATGGATCTATCGTGACTTGTGGGCACAGGTGGAAAAATATATTTTACATGAAGAACAATAATAAAC
  TCCCCACTGGTGTTTGCTATGGCATGCCTTCTGATTTGCGGACAGAACTAAGTAAAAGGATGGCCCCCTG
  TTACCAAGATTTTGTGAGAAAATTTGGAGAAAATTTTGCATCGTGTCAAGCTGGAATATCTAGTTTTTAC
  ACACAGGATTTGATTGTGATGGGGGCCCCGGGATCATCGTACTGGACCGGCTCCGTCTTTGTCTACAACA
  TAACTACGAACAAATACAAGGCATTTGTAGACAGACAGAATCAAGTAAAATTTGGAAGCTACTTAGGCTA
  CTCAGTAGGAGCTGGTCATTTCCGAAATCCACATACTACCGAAGTCGTGGGAGGAGCCCCTCAACACGAA
  CAGATAGGAAAAGCATATATATTCAGCATTGATGAAAGCGAACTGACCATCGTATATGAAATGAAAGGTA
  AAAAGCTTGGCTCTTACTTTGGAGCTTCTGTCTGTGCGGTGGACCTCAATGCAGATGGCTTCTCAGATCT
  CCTTGTTGGAGCGCCTATGCAGAGCACCATCAGGGAGGAAGGAAGAGTGTTCGTGTACATCAACTCTGGC
  ATGGGAGCTGTGATGGTTGAAATGGAAAGAGTGCTCGTCGGAAGTGACAAATATGCTGCCAGGTTTGGGG
  AATCCATGGCAAATCTTGGCGACATTGACAATGATGGCTTTGAAGATATTGCTATTGGCGCACCACAAGA
  AGATGACTTGCGAGGCGCTGTCTACATTTACAATGGCCGAGTAGATGGCATCTCCTCTACATACTCACAG
  AGAATCGAAGGACGTCAAATCAGCCAGTCACTAAGGATGTTTGGACAGTCTATCTCAGGACAAATTGATG
  CAGACAACAATGGATATGTTGATGTAGCGGTTGGTGCATTTCAGTCTGATTCTGCAGTGTTGCTAAGGAC
  ACGGCCTGTAGTGATTGTTGAAGCATCTTTAAGCCATCCTGAGTCAGTAAATAGAACAAAATTTGACTGT
  ACTGAAAATGGACTTCTGTCTGTGTGCGTGGATCTTAAACTGTGTTTCTCATATAAGGGCAAAGAGGTCC
  CAGGCTACATCGTTTTGTTTTACAATGTGAGCTTGGATGTGCACAGGAAGGCCGAGTCTCCGTCAAGATT
  TTATTTCTTCTCCAATGGGACTTCTGACATGATTACAAGAAGCATACGAGTTTCAAGCAGTGGAGAGAAA
  TGTAGGACACACCAGGCATTCATGCGGAAAGACGTGCGAGACATCCTCACTCCCATTCAGGTTGAGGCCA
  CATACCACCTTGGGCATCATGTGATCACCAAACGAAACACAGAGGAATTCCCACCCCTCCAGCCGATCCT
  TCAGCAGAAGAAAGAAAAAGACATTATTAGAAAAACGATAAACTTCGCAAGGTTTTGTGCCTATGAAAAT
  TGTTCTGCTGATCTGCAGGTTTCTGCAAAAGTTGGGTTTTTGAAGCCATATGAAAATAAAACCTATCTTG
  CTGTTGGGAGCATGAAGACCATAATGCTAAATGTGTCCTTGTTCAATGCTGGAGATGATGCTTATGAAAC
  TACTCTGAATGTCCAACTCCCCACAGGCCTTTATTTCATTAAGATCTTAGACCTGGAAGAGAAACAAATA
  AACTGCGACGTGACAGAGAGCTCAGGCGTAGTTAAGCTTGCCTGCAGCCTCGGTTACATATACGTGGATC
  GTCTCTCAAGGATAGACATTAGCTTTCTCCTGGATGTGAGCTCACTCAGCAGGGCCCATGAGGACCTCAG
  CATCAACGTGCATGCCTCCTGTGAAAATGAGGGTAAATTGGACCAAGTGAAAGACAACAGAGTGACCTTA
  GTGATACCTCTAAGGTATGAAGTTATGCTGGCTGTTCACGGGTTTGTGACCCCAACTTCGTTTGTGTATG
  GATCCAGTGAAGAAAACGAGCCAGAAATGTGCATGGCAGAGAAATTGAACCTCACTTTCCATGTTATAAA
  CACTGGCATTAGCATGGCTCCAAATGTTAGTGTGAAAATAATGGTACCAAATTCTTTTCTCCCTCGAAAT
  GATAAGTTGTTCAACGTTTTGGATGTCCAGGCAACTATGGGGCAGTGCCAATTTGAACACTATGGACGAG
  AGTGTGCATTAGCACAGCAAAAAGGCATAGCGGGGACCTTGACCGATTTGGTCAAATTTCTATCAAAGAC
  TGATAAGAGACTCCTGTATTGCATGAAAGCTGATCGACACTGTTTAGATTTCTTATGTAATTTCGGGAAA
  ATGGAAAGTGGGAAGGAAGCCAGCGTTCACATTCAGCTGGAAGGCAGGCCATCCGTCTTGGAAATGGATG
  AGACCTCATCGCTCAAATTTGAAATAAAAGCAACAGCTTTTCCAGAGCCACACCCAAAAGTTATTGAGCT
  CAATAAGGATGAGAACATTGCCCATGTTTACTTGGAAGGGCTCCATCATCAAAGACCCAAACGGCATTTC
  ACCATCGTGATTGTCACCATCAGCTTGCTCCTTGGACTTATTGTACTTTTATTAATTTCATGTGTTATGT
  GGAAGGCTGGATTTTTTAAAAGGCAGTACAAATCTATCCTACAAGAAGAAAACAGGAGAGACAGCTGGAG
  TTATGTCAACAGCAAAAGCAATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203360 representing NM_001107737
  Red=Cloning site Green=Tags(s)
  
  MAAEAMCRPRPRGVALQEAVMLLLYFGVHFRVPTGHSYNLDPESALLYQGPSGTLFGYSVVLHSHGTKRW
  LIVGAPTASWLANASVVNPGAIYRCRIGKNPNQICEQLQLGSPSGEPCGKTCLEERDNQWLGVTISRQPG
  ENGSIVTCGHRWKNIFYMKNNNKLPTGVCYGMPSDLRTELSKRMAPCYQDFVRKFGENFASCQAGISSFY
  TQDLIVMGAPGSSYWTGSVFVYNITTNKYKAFVDRQNQVKFGSYLGYSVGAGHFRNPHTTEVVGGAPQHE
  QIGKAYIFSIDESELTIVYEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSG
  MGAVMVEMERVLVGSDKYAARFGESMANLGDIDNDGFEDIAIGAPQEDDLRGAVYIYNGRVDGISSTYSQ
  RIEGRQISQSLRMFGQSISGQIDADNNGYVDVAVGAFQSDSAVLLRTRPVVIVEASLSHPESVNRTKFDC
  TENGLLSVCVDLKLCFSYKGKEVPGYIVLFYNVSLDVHRKAESPSRFYFFSNGTSDMITRSIRVSSSGEK
  CRTHQAFMRKDVRDILTPIQVEATYHLGHHVITKRNTEEFPPLQPILQQKKEKDIIRKTINFARFCAYEN
  CSADLQVSAKVGFLKPYENKTYLAVGSMKTIMLNVSLFNAGDDAYETTLNVQLPTGLYFIKILDLEEKQI
  NCDVTESSGVVKLACSLGYIYVDRLSRIDISFLLDVSSLSRAHEDLSINVHASCENEGKLDQVKDNRVTL
  VIPLRYEVMLAVHGFVTPTSFVYGSSEENEPEMCMAEKLNLTFHVINTGISMAPNVSVKIMVPNSFLPRN
  DKLFNVLDVQATMGQCQFEHYGRECALAQQKGIAGTLTDLVKFLSKTDKRLLYCMKADRHCLDFLCNFGK
  MESGKEASVHIQLEGRPSVLEMDETSSLKFEIKATAFPEPHPKVIELNKDENIAHVYLEGLHHQRPKRHF
  TIVIVTISLLLGLIVLLLISCVMWKAGFFKRQYKSILQEENRRDSWSYVNSKSNDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107737
• ORF Size: 3108 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107737.1, NP_001101207.1
• RefSeq Size: 3736 bp
• RefSeq ORF: 3111 bp
• Locus ID: 311144
• Cytogenetics: 3q24
• MW: 115.6 kDa
• Gene Summary: mouse homolog is involved in adult hematopoietic cell homeostasis, regeneration, and homing [RGD, Feb 2006]