Rnf31 (NM_001108868) Rat Tagged ORF Clone

CAT#: RR203315

Rnf31 (Myc-DDK-tagged ORF) - Rat ring finger protein 31 (Rnf31), (10 ug)

Product Images

Circular map for RR203315

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203315 representing NM_001108868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGAGACGAGGAGCGAGCCTTCCTAGCGGCCCGCGAGGAGCTGGCGAGCGCCCTGAGGTGGGATT
  CTGCGCAGGTATTTCCCCTGGAGCAGCTCACGCCGCTTCTGGCCACCTCTCTGCCACTCTCCTCCCGCTA
  CCGGCAGCTGGATGCCGGACGCCTGGTCCGCTGCAACGCTCATGGGGAGCCTCGAAACTACCTCAACACT
  CTATCCACGGCCCTGAACATCCTGGAAAAGTATGGTCGAAACCTCCTCAGCCCACAGCGGCCCCGGTATT
  GGCGCTCAGTGAAGTTTAATAACCCTGTCTTTCGCAGCACGGTGGATGCTGTGCAGGGTGGCCGGGATGT
  GCTACGGTTGTATGGCTATACCGAGGAGCGCCCAGATGGATTGAGTTTCCCCGGAGGGCAGGAGGAACCA
  GATGAATACCAGGTTGCCATAGTCACACTAGAAGTACTACTGCTTCGCACGGAGCTCAATTTGCTGTTGC
  AGAATACTCATCCAAGACAGAACGCACTGGACCAGCTACTAAGAGACAGCGTTGAAGATGACATGCTGCA
  GCTTTCAGAGTTTCACCCCCTTTTGAGAGAGATTGTTCCTGGCCCCCTCCCCTCTGCCCCAGGCCATACT
  CCTGGTCCCTGCTTTCTCTGTGGTTCTGCCCCAGGCACACTGCACTGTCCAGCCTGTAACCAAGCTTCAT
  GCCCAGCTTGTGACCTTTTGTTCCATGGGCATCCGTCCCGTGCGCATCACCTTCGCCACACCCTACCCGG
  GGCCCACCAGACCACCAACCTAAGCTCTAGTTTACCTGCCTCGTCCCAACCACGGCCCCACTCCTCCTCC
  CTGGCCATGGGAGATAGCTCTCTCTCTTCCCCTGACCCTGCAAATGCTTGTCTGCCCTGGCACTGTCCTA
  CCTGTGCCACATTAAACGAGCCTTGGGCAGTGTTCTGTGCAGCCTGTAGTCAGCCCAAAGGCTGTAAGGT
  GCGGGGAAGAGAGAGTTCCCAAGGAACTGGGGTCCTAGAGCCCGAGCCTGCACGGGATCAGTGGGCCTGC
  CAGAGCTGTACCTTTGAGAATGAGGCAGCAGCCGTGCTCTGTGCCATCTGTGAGCGACCTCGGCTGGCCC
  AGCCTCCCAGCTTGGTGGTGGATTCCCATGACGCTGGTGTTTACCAACAGCCCCTTAAGCAGGAGGATAC
  TTTGCTCTCCGCTGCCCAGTCTCAGGTGTGGCACTGTGACCATTGTACCTTCTGCAATTCAGGCCCTGTC
  TGGGTGTGTGCCATGTGTAACCGGACTCGCAGCCCCATCCCTGCACAGCCCTATCCCAGCTCCTTGGAGA
  AGGGACTCTCACAGCCAGGGTCCTCACAACACCTCAGCTCCTCCCTGCCTGCTTCCTGTGGAGATCCAGA
  GAAACAGCGCCAAGATAAGATGCGGAAGGAAAGTCTCCAGCTAGTGAACATGATCCAGGAAGGGGAAGCT
  GCAGGTGCCAGTCCAGAAGAGATCTTCTCGGCTCTACAATACTCGGGCACTGAGGTGCCCCTGCAGTGGT
  TGCGTTCAGAGCTGTCCTACGTCTTGGAGATGGTGGCTGAGCTAGCTGGACAACGGGATCCAGAGCTCGG
  TGCCTTTTCCTGTCAGGAAGCCCGGAAAGCCTGGCTTGATCGTCATGGCAACCTTGATGAAGCTGTAGAG
  GAGTGTGTGAGGGCCAGGAGGAGGAAGGTGCAGGAGCTCCAGTCCCTGGGCTTTGAGCCTAAAGAAGGGT
  CGCTGCAGGCATTGTTCCAGCATGGGGGTGATGTGGCCCGGGCCCTAACTGAGTTACAGCGACAGCGCCT
  GGAGCCTTTCCACCAGCGCCTCTGGGGCAGAGACCCCGAACCCACTCCCTGCTGGGATGGCCTGGATAGA
  CAGAGCCTGGTCAGACGCCTTCTGGCGGTATACACACTCCCCAGCTGGGGTCGAGCAGAGTTGGCGTTAG
  CGCTGCTGCAGGAGACACCCAGGAACTATGAGTTGTTGGACGTGGTGGAGGCCGTGAGGCACAGTCAGGA
  CCGGGCCTTTCTGCGCCGACTGCTTGCCCAGGAATGTGCTGTGTGCGGGTGGGCCCTTCCCCGAAACCGG
  ATGCAGGCCCTGATCTCCTGCGAGTGCACCATATGCCCTGAGTGTTTCCGCCAGCATTTCACCATTGCCC
  TGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCCTGACCTCACCGATGATGCGCA
  GTTACTCAGTTACTTCTCCACCCTTGACATCCAGCTCAGAGAGAGCCTAGAGCCGGATGCATATGCTCTG
  TTTCATAAGAAGCTAACCGAGGCTGTGCTCATGCGAGACCCCAAGTTCTTGTGGTGTGCCCAGTGTTCCT
  TCGGCTTCATATATGAACGAGAACAGCTGGAGGCGACATGTCCCCAGTGTCACCAGACCTTCTGTGTGCG
  CTGCAAGCGCCAGTGGGAGGAGCAGCACAGAGGGCGGAGCTGTGAGGATTTCCAGAACTGGAAACGCACC
  AATGACCCAGAGTACCAGGCTCAAGGCCTGGCCATGTACCTTCAGGAAAACGGCATAGACTGCCCCAAAT
  GCAAGTTCTCATACGCACTGGCCCGAGGAGGCTGCATGCACTTCCACTGCACACAGTGTCGCCACCAGTT
  CTGCAGCGGCTGCTACAATGCCTTTTACGCCAAGAATAAATGTCCAGACCCTAACTGCAAGGTGAAAAAG
  TCCCTGCATGGTCACCACCCTCGAGATTGCCTCTTCTACCTACGGGATTGGCCTGCTGCCCGGCTCCAGA
  AACTTTTGCAGGACAATAATGTCATGTTTAACACAGAGCCTCCTGCCGGCACACGGGCAGTCCCTGGAGG
  TGGCTGCCGAGTGATGGAACAGAAGGAGGTTCCCAGTGGGTTCAGGGATGAAGCTTGTGGCAAGGAAACT
  CCGCCTGGCTATGCCGGCCTGTGTCAGGCACACTACAAAGAGTATCTTGTGAGCCTCATCAATGCCCACT
  CGCTGGACCCAGCGACCCTGTATGAAGTGGAGGAACTGGAGACAGCCACTGCACGCTACCTACATTTAAC
  GCCCCAACCCATGGATGGAGAGGATCTTCCTGCTTACCAGGCCCGGTTGTTACAGAAGCTGAGAGAAGAG
  GTGCCCTTGGGACAGAGTATTGCTCGCAGAAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203315 representing NM_001108868
  Red=Cloning site Green=Tags(s)
  
  MPGDEERAFLAAREELASALRWDSAQVFPLEQLTPLLATSLPLSSRYRQLDAGRLVRCNAHGEPRNYLNT
  LSTALNILEKYGRNLLSPQRPRYWRSVKFNNPVFRSTVDAVQGGRDVLRLYGYTEERPDGLSFPGGQEEP
  DEYQVAIVTLEVLLLRTELNLLLQNTHPRQNALDQLLRDSVEDDMLQLSEFHPLLREIVPGPLPSAPGHT
  PGPCFLCGSAPGTLHCPACNQASCPACDLLFHGHPSRAHHLRHTLPGAHQTTNLSSSLPASSQPRPHSSS
  LAMGDSSLSSPDPANACLPWHCPTCATLNEPWAVFCAACSQPKGCKVRGRESSQGTGVLEPEPARDQWAC
  QSCTFENEAAAVLCAICERPRLAQPPSLVVDSHDAGVYQQPLKQEDTLLSAAQSQVWHCDHCTFCNSGPV
  WVCAMCNRTRSPIPAQPYPSSLEKGLSQPGSSQHLSSSLPASCGDPEKQRQDKMRKESLQLVNMIQEGEA
  AGASPEEIFSALQYSGTEVPLQWLRSELSYVLEMVAELAGQRDPELGAFSCQEARKAWLDRHGNLDEAVE
  ECVRARRRKVQELQSLGFEPKEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWGRDPEPTPCWDGLDR
  QSLVRRLLAVYTLPSWGRAELALALLQETPRNYELLDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNR
  MQALISCECTICPECFRQHFTIALKEKHITDMVCPACGRPDLTDDAQLLSYFSTLDIQLRESLEPDAYAL
  FHKKLTEAVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRT
  NDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCKVKK
  SLHGHHPRDCLFYLRDWPAARLQKLLQDNNVMFNTEPPAGTRAVPGGGCRVMEQKEVPSGFRDEACGKET
  PPGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATARYLHLTPQPMDGEDLPAYQARLLQKLREE
  VPLGQSIARRRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001108868
• ORF Size: 3186 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001108868.1, NP_001102338.2
• RefSeq Size: 3394 bp
• RefSeq ORF: 3189 bp
• Locus ID: 364386
• Cytogenetics: 15p13
• MW: 119.2 kDa