Nr3c1 (NM_012576) Rat Tagged ORF Clone

CAT#: RR203250

Nr3c1 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 3, group C, member 1 (Nr3c1), (10 ug)

Product Images

Circular map for RR203250

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr3c1
• Synonyms: Gcr; GR; Grl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203250 representing NM_012576
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCAAAGAATCCTTAGCTCCCCCTGGTAGAGACGAAGTCCCTGGCAGTTTGCTTGGCCAGGGGA
  GGGGGAGCGTAATGGACTTTTATAAAAGCCTGAGGGGAGGAGCTACAGTCAAGGTTTCTGCATCTTCGCC
  CTCAGTGGCTGCTGCTTCTCAGGCAGATTCCAAGCAGCAGAGGATTCTCCTTGATTTCTCGAAAGGCTCC
  ACAAGCAATGTGCAGCAGCGACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGC
  AGCAGCCAGACTTATCCAAAGCCGTTTCACTGTCCATGGGGCTGTATATGGGAGAGACAGAAACAAAAGT
  GATGGGGAATGACTTGGGCTACCCACAGCAGGGCCAACTTGGCCTTTCCTCTGGGGAAACAGACTTTCGG
  CTTCTGGAAGAAAGCATTGCAAACCTCAATAGGTCGACCAGCGTTCCAGAGAACCCCAAGAGTTCAACGT
  CTGCAACTGGGTGTGCTACCCCGACAGAGAAGGAGTTTCCCAAAACTCACTCGGATGCATCTTCAGAACA
  GCAAAATCGAAAAAGCCAGACCGGCACCAACGGAGGCAGTGTGAAATTGTATCCCACAGACCAAAGCACC
  TTTGACCTCTTGAAGGATTTGGAGTTTTCCGCTGGGTCCCCAGGTAAAGACACAAACGAGAGTCCCTGGA
  GATCAGACCTGTTGATAGATGAAAACTTGCTTTCTCCTTTGGCGGGAGAAGATGATCCATTCCTTCTCGA
  AGGGGACACGAATGAGGATTGTAAGCCTCTTATTTTACCGGACACTAAACCTAAAATTAAGGATACTGGA
  GATACAATCTTATCAAGTCCCAGCAGTGTGGCACTGCCCCAAGTGAAAACAGAAAAAGATGATTTCATTG
  AACTTTGCACCCCCGGGGTAATTAAGCAAGAGAAACTGGGCCCAGTTTATTGTCAGGCAAGCTTTTCTGG
  GACAAATATAATTGGTAATAAAATGTCTGCCATTTCTGTTCATGGTGTGAGTACCTCTGGAGGACAGATG
  TACCACTATGACATGAATACAGCATCCCTTTCTCAGCAGCAGGATCAGAAGCCTGTTTTTAATGTCATTC
  CACCAATTCCTGTTGGTTCTGAAAACTGGAATAGGTGCCAAGGCTCCGGAGAGGACAGCCTGACTTCCTT
  GGGGGCTCTGAACTTCCCAGGCCGGTCAGTGTTTTCTAATGGGTACTCAAGCCCTGGAATGAGACCAGAT
  GTAAGCTCTCCTCCATCCAGCTCGTCAGCAGCCACGGGACCACCTCCCAAGCTCTGCCTGGTGTGCTCCG
  ATGAAGCTTCAGGATGTCATTACGGGGTGCTGACATGTGGAAGCTGCAAAGTATTCTTTAAAAGAGCAGT
  GGAAGGACAGCACAATTACCTTTGTGCTGGAAGAAACGATTGCATCATTGATAAAATTCGAAGGAAAAAC
  TGCCCAGCATGCCGCTATCGGAAATGTCTTCAGGCTGGAATGAACCTTGAAGCTCGAAAAACAAAGAAAA
  AAATCAAAGGGATTCAGCAAGCCACTGCAGGAGTCTCACAAGACACTTCGGAAAATCCTAACAAAACAAT
  AGTTCCTGCAGCATTACCACAGCTCACCCCTACCTTGGTGTCACTGCTGGAGGTGATTGAACCCGAGGTG
  TTGTATGCAGGATATGATAGCTCTGTTCCAGATTCAGCATGGAGAATTATGACCACACTCAACATGTTAG
  GTGGGCGTCAAGTGATTGCAGCAGTGAAATGGGCAAAGGCGATACCAGGCTTCAGAAACTTACACCTGGA
  TGACCAAATGACCCTGCTACAGTACTCATGGATGTTTCTCATGGCATTTGCCCTGGGTTGGAGATCATAC
  AGACAATCAAGTGGAAACCTGCTCTGCTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGTCTCTAC
  CCTGCATGTATGACCAATGTAAACACATGCTGTTTGTCTCCTCTGAATTACAAAGATTGCAGGTATCCTA
  TGAAGAGTATCTCTGTATGAAAACCTTACTGCTTCTCTCCTCAGTTCCTAAGGAAGGTCTGAAGAGCCAA
  GAGTTATTTGATGAGATTCGAATGACTTATATCAAAGAGCTAGGAAAAGCCATCGTCAAAAGGGAAGGGA
  ACTCCAGTCAGAACTGGCAACGGTTTTACCAACTGACAAAGCTTCTGGACTCCATGCATGAGGTGGTTGA
  GAATCTCCTTACCTACTGCTTCCAGACATTTTTGGATAAGACCATGAGTATTGAATTCCCAGAGATGTTA
  GCTGAAATCATCACTAATCAGATACCAAAATATTCAAATGGAAATATCAAAAAGCTTCTGTTTCATCAAA
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203250 representing NM_012576
  Red=Cloning site Green=Tags(s)
  
  MDSKESLAPPGRDEVPGSLLGQGRGSVMDFYKSLRGGATVKVSASSPSVAAASQADSKQQRILLDFSKGS
  TSNVQQRQQQQQQQQQQQQQQQQQQPDLSKAVSLSMGLYMGETETKVMGNDLGYPQQGQLGLSSGETDFR
  LLEESIANLNRSTSVPENPKSSTSATGCATPTEKEFPKTHSDASSEQQNRKSQTGTNGGSVKLYPTDQST
  FDLLKDLEFSAGSPGKDTNESPWRSDLLIDENLLSPLAGEDDPFLLEGDTNEDCKPLILPDTKPKIKDTG
  DTILSSPSSVALPQVKTEKDDFIELCTPGVIKQEKLGPVYCQASFSGTNIIGNKMSAISVHGVSTSGGQM
  YHYDMNTASLSQQQDQKPVFNVIPPIPVGSENWNRCQGSGEDSLTSLGALNFPGRSVFSNGYSSPGMRPD
  VSSPPSSSSAATGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKN
  CPACRYRKCLQAGMNLEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEV
  LYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSY
  RQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQ
  ELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEML
  AEIITNQIPKYSNGNIKKLLFHQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012576
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012576.2, NP_036708.2
• RefSeq Size: 6327 bp
• RefSeq ORF: 2385 bp
• Locus ID: 24413
• UniProt ID: P06536
• Cytogenetics: 18p11
• MW: 87.4 kDa
• Gene Summary: glucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]