Aco1 (NM_017321) Rat Tagged ORF Clone

CAT#: RR203228

Aco1 (Myc-DDK-tagged ORF) - Rat aconitase 1, soluble (Aco1), (10 ug)

Product Images

Circular map for RR203228

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aco1
• Synonyms: Acon1; AH; IRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203228 representing NM_017321
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATCCATTTGCGCACCTTGCCGAGCCCTTGGACCCTGCACAGCCAGGAAAGAAATTCTTCAACT
  TGAATAAATTGGAGGACTCAAGATATGGACGCTTACCGTTCTCTATCAGAGTTCTCCTGGAGGCCGCCGT
  TCGGAACTGTGACGAGTTTTTGGTGAAGAAGAATGACATTGAGAATATCCTGAACTGGAGTATCATGCAG
  CATAAGAGCATAGAAGTGCCGTTTAAGCCAGCCCGAGTCATACTGCAGGACTTTACGGGCGTGCCTGCTG
  TGGTTGATTTTGCAGCAATGCGCGATGCTGTGAAGAAGTTGGGAGGAAATCCAGAGAAAATAAACCCTGT
  CTGCCCCGCTGACCTTGTAATCGATCATTCCATCCAGGTTCATTTCAACAGAAGGGCAGACAGTTTGCAG
  AAGAATCAAGACCTGGAATTTGAAAGGAATAGAGAACGATTTGAATTTTTAAAGTGGGGTTCCCAGGCCT
  TTTGCAACATGAGGATTATTCCTCCTGGCTCAGGAATTATTCACCAAGTGAATCTGGAGTATTTGGCAAG
  AGTAGTGTTTGATCAGGATGGATGTTACTACCCAGATAGCCTCGTGGGCACAGATTCTCACACCACCATG
  ATTGATGGTCTGGGAGTTCTTGGTTGGGGTGTAGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGC
  CAATCAGCATGGTGCTTCCCCAGGTGATTGGCTACAAGCTGATGGGGAAGCCTCACCCTCTGGTAACCTC
  CACGGACATTGTGCTCACCATTACCAAGCACCTCCGACAAGTTGGGGTCGTGGGCAAATTCGTGGAGTTT
  TTCGGGCCAGGAGTGGCCCAGCTGTCCATTGCTGACCGAGCTACGATTGCCAACATGTGCCCAGAGTACG
  GCGCGACGGCAGCCTTCTTCCCGGTCGACGACGTTAGCATCGCGTACCTGGTGCAGACAGGTCGTGAGGA
  AGACAAAGTAAAGCACATTAAAAGGTATCTTCAGGCTGTAGGCATGTTTCGAGACTTCAGTGACTCCTCT
  CAAGACCCAGACTTCACTCAGGTCGTGGAGTTAGATTTGAAAACAGTTGTGCCTTGCTGCAGTGGACCTA
  AAAGACCTCAGGACAAAGTTGCTGTGTCTGAGATTGAAAAGGACTTTGAAAGCTGCCTTGGAGCCAAGCA
  AGGATTTAAAGGTTTTCAAGTTGCTCCAGACCATCACAATGACCACAAGACGTTTATCTATAACGACAGT
  GAATTCACTCTTGCTCATGGCTCCGTGGTGATCGCCGCCATCACTAGCTGCACAAACACCAGCAATCCGT
  CCGTGATGTTAGGCGCAGGATTGTTAGCAAAGAAAGCCGTAGAGGCTGGCCTGAATGTGAAGCCTTACGT
  CAAAACCAGCCTGTCTCCTGGGAGTGGAGTGGTCACCTACTACCTTCGAGAGAGTGGAGTCATGCCTTAC
  CTGTCCCAGTTAGGGTTTGACGTGGTGGGCTACGGCTGCATGACCTGCATCGGCAACAGTGGACCCCTCC
  CCGAACCTGTGGTGGAGGCTATCACCCAGGGAGACCTTGTGGCTGTTGGGGTACTGTCTGGAAACAGGAA
  TTTTGAAGGACGAGTCCATCCTAACACCCGGGCCAACTACTTAGCATCTCCCCCACTAGTAATAGCATAT
  GCAATTGCAGGCACCGTCAGGATCGACTTCGAGAAAGAGCCTTTGGGAGTGAACGCACAGGGCCAACAAG
  TGTTTCTGAAGGATATCTGGCCAACTCGAGATGAGATCCAGGAGGTGGAGCGGAAGTATGTCATCCCCGG
  CATGTTCAAGGAGGTCTATCAGAAGATAGAGACTGTAAACAAAAGCTGGAATGCCTTAGCAGCCCCTTCA
  GAGAAGCTGTATGCGTGGAACCCCAAGTCTACTTATATCAAGTCACCGCCATTCTTTGAAAGCTTGACTT
  TAGATCTCCAGCCCCCCAAGTCTATAGTGGATGCCTATGTGCTACTAAATCTAGGAGATTCCGTAACAAC
  GGACCATATCTCTCCAGCGGGGAACATTGCAAGAAACAGCCCTGCCGCTCGCTACTTGACGAACAGAGGC
  CTGACGCCACGAGATTTCAACTCCTACGGCTCCCGCCGGGGTAACGACGCCATCATGGCACGGGGGACAT
  TTGCCAACATTCGCTTGCTGAACAAGTTTCTGAACAAGCAGGCCCCTCAGACTGTCCACCTTCCTTCAGG
  AGAAACCCTCGATGTGTTCGATGCCGCTGAGCGGTACCAGCAGGCTGGACTTCCCCTGATTGTTCTGGCT
  GGCAAAGAGTACGGTTCAGGCAGCTCCCGAGACTGGGCAGCCAAAGGTCCTTTCCTGCTGGGAATCAAAG
  CTGTCCTGGCAGAGAGCTACGAGCGCACTCACTGCAGCAACCTGGTTGGCATGGGGGTGATCCCCCTTGA
  GTATCTCCCCGGCGAAACTGCAGACTCTCTGGGACTCACGGGTCGGGAAAGGTACACGATCCACATTCCC
  GAACACCTTAAGCCCCGCATGAAGGTTCAGATAAAGCTGGACACCGGGAAGACCTTCCAGGCCGTGATGA
  GGTTCGACACCGACGTGGAGCTCACTTACTTCCACAATGGAGGCATCCTGAACTACATGATCCGAAAGAT
  GGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203228 representing NM_017321
  Red=Cloning site Green=Tags(s)
  
  MKNPFAHLAEPLDPAQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAVRNCDEFLVKKNDIENILNWSIMQ
  HKSIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGNPEKINPVCPADLVIDHSIQVHFNRRADSLQ
  KNQDLEFERNRERFEFLKWGSQAFCNMRIIPPGSGIIHQVNLEYLARVVFDQDGCYYPDSLVGTDSHTTM
  IDGLGVLGWGVGGIEAEAVMLGQPISMVLPQVIGYKLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEF
  FGPGVAQLSIADRATIANMCPEYGATAAFFPVDDVSIAYLVQTGREEDKVKHIKRYLQAVGMFRDFSDSS
  QDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSEIEKDFESCLGAKQGFKGFQVAPDHHNDHKTFIYNDS
  EFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLNVKPYVKTSLSPGSGVVTYYLRESGVMPY
  LSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAY
  AIAGTVRIDFEKEPLGVNAQGQQVFLKDIWPTRDEIQEVERKYVIPGMFKEVYQKIETVNKSWNALAAPS
  EKLYAWNPKSTYIKSPPFFESLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRG
  LTPRDFNSYGSRRGNDAIMARGTFANIRLLNKFLNKQAPQTVHLPSGETLDVFDAAERYQQAGLPLIVLA
  GKEYGSGSSRDWAAKGPFLLGIKAVLAESYERTHCSNLVGMGVIPLEYLPGETADSLGLTGRERYTIHIP
  EHLKPRMKVQIKLDTGKTFQAVMRFDTDVELTYFHNGGILNYMIRKMAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017321
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017321.1, NP_059017.1
• RefSeq Size: 3564 bp
• RefSeq ORF: 2670 bp
• Locus ID: 50655
• UniProt ID: Q63270
• Cytogenetics: 5q22
• MW: 98.1 kDa
• Gene Summary: This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism [provided by RefSeq, Jan 2014]