Tgfb2 (NM_031131) Rat Tagged ORF Clone

CAT#: RR203156

Tgfb2 (Myc-DDK-tagged ORF) - Rat transforming growth factor, beta 2 (Tgfb2), (10 ug)

Product Images

Circular map for RR203156

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tgfb2
• Synonyms: TGF-B2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203156 representing NM_031131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTACTGTGTGCTGAGAACCTTTTTGCTCCTGCATCTGGTCCCGGTGGCGCTCAGTCTGTCTACCT
  GCAGCACCCTCGACATGGACCAGTTTATGCGCAAGAGGATCGAGGCCATCCGCGGGCAGATCCTGAGCAA
  GCTGAAGCTCACCAGCCCCCCGGAAGACTATCCGGAGCCGGATGAGGTCCCCCCGGAGGTGATTTCCATC
  TACAACAGTACCAGGGACTTACTGCAGGAGAAGGCAAGCCGGAGGGCAGCCGCCTGCGAGCGCGAGCGAA
  GCGACGAGGAGTACTACGCCAAGGAGGTTTATAAAATCGACATGCCGTCCCACTTCCCCTCCGAAACTGT
  CTGCCCAGTTGTTACAACATCCTCTGGCTCAGTGGGCAGCTTTTGCTCCATACAGTCCCAGGTGCTCTGT
  GGGTACCTTGATGCCATCCCGCCCACTTTCTACAGACCCTACTTCAGAATCGTCCGCTTCGATGTCTCAA
  CAATGGAGAAGAATGCTTCAAATCTGGTGAAGGCAGAGTTCAGGGTCTTTCGCTTGCAGAACCCCAAGGC
  CAGAGTGGCTGAACAACGGATTGAACTGTATCAGATCCTTAAATCCAAAGACCTAACATCTCCAACCCAG
  CGCTACATTGATAGCAAGGTTGTGAAAACCAGAGCCGAGGGGGAATGGCTCTCCTTCGACGTGACAGACG
  CCGTGCACGAGTGGCTTCACCACAAAGACAGGAACCTGGGATTTAAAATAAGTTTACACTGCCCCTGCTG
  TACCTTCATACCGTCTAATAATTACATCATCCCAAATAAGAGCCAAGAGCTGGAGGCGAGATTCGCAGGT
  ATCGATGGCACCTCCACATATGCCAGTGGTGATCAGAAAACTATAAAGTCCACTAGGAAAAAAAGCAGTG
  GGAAGACCCCGCATCTCCTGCTAATGTTGTTGCCCTCCTACAGACTGGAGTCCCAGCAGTCCAGCCGGCG
  ACGGAAGCGGGCTTTGGATGCCGCCTATTGCTTTAGGAATGTGCAGGATAATTGCTGCCTTCGCCCTCTT
  TACATTGATTTTAAGAGGGATCTTGGATGGAAATGGATCCATGAACCCAAAGGATACAATGCTAACTTCT
  GTGCTGGGGCATGCCCTTATCTGTGGAGTTCAGACACACAACACACCAAAGTCCTCAGCCTGTACAACAC
  CATAAACCCCGAAGCTTCTGCTTCCCCTTGCTGTGTGTCCCAGGATCTGGAACCACTGACCATCCTCTAC
  TACATTGGCAATACGCCCAAGATCGAACAGCTTTCCAACATGATCGTCAAGTCTTGTAAATGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203156 representing NM_031131
  Red=Cloning site Green=Tags(s)
  
  MHYCVLRTFLLLHLVPVALSLSTCSTLDMDQFMRKRIEAIRGQILSKLKLTSPPEDYPEPDEVPPEVISI
  YNSTRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPSHFPSETVCPVVTTSSGSVGSFCSIQSQVLC
  GYLDAIPPTFYRPYFRIVRFDVSTMEKNASNLVKAEFRVFRLQNPKARVAEQRIELYQILKSKDLTSPTQ
  RYIDSKVVKTRAEGEWLSFDVTDAVHEWLHHKDRNLGFKISLHCPCCTFIPSNNYIIPNKSQELEARFAG
  IDGTSTYASGDQKTIKSTRKKSSGKTPHLLLMLLPSYRLESQQSSRRRKRALDAAYCFRNVQDNCCLRPL
  YIDFKRDLGWKWIHEPKGYNANFCAGACPYLWSSDTQHTKVLSLYNTINPEASASPCCVSQDLEPLTILY
  YIGNTPKIEQLSNMIVKSCKCS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031131
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031131.1, NP_112393.1
• RefSeq Size: 2880 bp
• RefSeq ORF: 1329 bp
• Locus ID: 81809
• UniProt ID: Q07257
• Cytogenetics: 13q26
• MW: 50.5 kDa
• Gene Summary: binds the transforming growth factor-beta receptor; plays a role in regulation of cell growth and proliferation; may be involved in mesenchymal-epithelial cell interactions during development [RGD, Feb 2006]