Krt7 (NM_001047870) Rat Tagged ORF Clone

CAT#: RR203147

Krt7 (Myc-DDK-tagged ORF) - Rat keratin 7 (Krt7), (10 ug)

Product Images

Circular map for RR203147

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Krt7
• Synonyms: Krt2-7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203147 representing NM_001047870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATCCACTTCAGCTCTCGCTCCACTGCTTACCCGGGCCGCGGGGCTCAGGTGCGCCTGAGCTCGG
  GCCGCGCCAGCTTCGGCAGCAGGAGCCTCTATGGCCTGGGCTCATCAAGGCCTCGTGTGGCCGTGCGCTC
  CGCTTATGGGGGCCCGGTGGGCGCTGGCATCCGCGAGATCACCATCAATCAGAGCTTGCTGGCGCCGCTG
  AGTGTGGACATCGATCCCACCATCCAGCAGGTGCGCCAGGAGGAGCGCGAGCAGATCAAGACCCTCAACA
  ACAAATTCGCCTCCTTCATCGACAAGGTACGCTTCCTGGAACAGCAGAACAAGATGCTGGAGACCAAGTG
  GGCGCTGCTGCAGGAACAGAAGTCAGCCAAGAGCAGCCAGCTTCCCCGAATCTTTGAGGCTCAGATTGCT
  GGGCTGAGGCAGCAGCTCGAGACACTGCAGCTGGATGGGGGCCGCCTGGAGGTGGAACTGCGGAACATGC
  AGGATGTGGTGGAAGATTTCAAGAATAAGTATGAAGAGGAGATCAACCGACGCACGGCTGCTGAGAATGA
  GTTTGTGTTGCTGAAGAAGGATGTGGATGCCGCCTACACGAACAAGGTGGAGTTGGAGGCCAAGGCGGAC
  AGTCTGCAGGATGAGATCAACTTCCTCAAGACCCTTCACGAGACAGAGTTAGCAGAGCTGCAGTCGCAGA
  TCTCAGACACATCTGTGGTGCTGTCCATGGACAACAGCCGCTCCCTGGACTTGGATGGTATCATTGCTGA
  CGTCAAAGCCCAGTATGAGGAGATGGCCAACCACAGCCGGGCAGAGGCTGAAGCTTGGTACCAGACCAAG
  TTTGAGACACTCCAGGCCCAGGCTGGGAAGCACGGGGATGACCTCCGCAACACCCGGAATGAGATTGCGG
  AGATGAACCGCTCTATCCAGAGGCTGCAGGCAGAGATTGACACCTTGAAGAACCAGCGTGCCAAGTTAGA
  GTCCAGCATCGCAGAGGCTGAGGAACAAGGGGAGCTGGCAATCAAGGATGCTCATGCTAAGCAGGGGGAG
  CTGGAGGCTGCCCTGCAGAAGGCCAAGCAGGATGTGGCCCGGCAACTTCGAGAGTACCAGGAGCTCCTGA
  ACACCAAGCTGGCGCTGGACATTGAGATCGCCACCTACCGCAAGCTGCTGGAGGGCGAGGAGAGCCGGTT
  GTCTGGAGACGGAATGGGACCTGTGAATATCTCTGTTGTGAATTCCACTGGGGGCAATGGTGGCAAGCTT
  ATCTTCGGAGGAACCATGGGAAGCAATGCTCTGAGCTTCAGTGGGGGACCCGGAGCCCTCAGGGCCTATT
  CCATCAAGACCACATCTACTACCCGCAGGGGCACCCACAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203147 representing NM_001047870
  Red=Cloning site Green=Tags(s)
  
  MSIHFSSRSTAYPGRGAQVRLSSGRASFGSRSLYGLGSSRPRVAVRSAYGGPVGAGIREITINQSLLAPL
  SVDIDPTIQQVRQEEREQIKTLNNKFASFIDKVRFLEQQNKMLETKWALLQEQKSAKSSQLPRIFEAQIA
  GLRQQLETLQLDGGRLEVELRNMQDVVEDFKNKYEEEINRRTAAENEFVLLKKDVDAAYTNKVELEAKAD
  SLQDEINFLKTLHETELAELQSQISDTSVVLSMDNSRSLDLDGIIADVKAQYEEMANHSRAEAEAWYQTK
  FETLQAQAGKHGDDLRNTRNEIAEMNRSIQRLQAEIDTLKNQRAKLESSIAEAEEQGELAIKDAHAKQGE
  LEAALQKAKQDVARQLREYQELLNTKLALDIEIATYRKLLEGEESRLSGDGMGPVNISVVNSTGGNGGKL
  IFGGTMGSNALSFSGGPGALRAYSIKTTSTTRRGTHN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047870
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047870.1, NP_001041335.1
• RefSeq Size: 1374 bp
• RefSeq ORF: 1374 bp
• Locus ID: 300242
• UniProt ID: Q6IG12
• Cytogenetics: 7q36
• MW: 50.7 kDa
• Gene Summary: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells.[UniProtKB/Swiss-Prot Function]