Cttn (NM_021868) Rat Tagged ORF Clone

CAT#: RR203142

Cttn (Myc-DDK-tagged ORF) - Rat cortactin (Cttn), (10 ug)

Product Images

Circular map for RR203142

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cttn
• Synonyms: Cttnb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR203142 representing NM_021868
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAAGCTTCTGCAGGCCATGCTGTGTCCATCACCCAGGATGATGGGGGAGCCGATGACTGGGAGA
  CTGACCCTGATTTTGTGAACGATGTGAGTGAAAAGGAACAGAGATGGGGCGCCAAAACCGTGCAGGGATC
  GGGGCACCAGGAACACATCAACATACACAAGCTTCGAGAGAATGTCTTCCAAGAACACCAGACACTCAAG
  GAGAAGGAGCTGGAAACGGGACCCAAGGCTTCCCATGGCTATGGCGGGAAGTTTGGTGTGGAGCAAGATA
  GGATGGACAAATCAGCTGTGGGCCATGAGTACCAGTCAAAGCTCTCCAAGCACTGCTCACAAGTGGACTC
  AGTCCGGGGCTTCGGAGGCAAGTTCGGTGTCCAGATGGACAGGGTGGATCAGTCTGCTGTAGGCTTTGAA
  TACCAGGGGAAGACGGAGAAGCACGCCTCCCAGAAAGACTACTCCAGTGGTTTCGGTGGCAAATATGGAG
  TGCAAGCTGACCGTGTAGACAAGAGTGCTGTGGGCTTTGACTACCAGGGCAAGACGGAGAAGCACGAGTC
  ACAGAAAGATTACTCCAAAGGTTTTGGTGGCAAATACGGAATTGACAAGGACAAGGTGGATAAAAGCGCT
  GTGGGCTTTGAGTATCAAGGCAAAACAGAGAAGCATGAGTCCCAGAAAGACTATGTAAAAGGCTTTGGAG
  GAAAGTTTGGTGTGCAGACAGACAGACAGGACAAGTGTGCCCTTGGCTGGGACCATCAGGAGAAGCTGCA
  GCTGCATGAATCCCAAAAAGACTATGCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATG
  GACAAGAACGCATCCACCTTTGAAGAAGTGGTCCAGGTGCCGTCTGCCTATCAGAAGACTGTCCCCATTG
  AGGCTGTGACCAGCAAAACCAGTAATATTCGTGCTAACTTTGAAAACCTGGCAAAGGAGAGAGAGCAGGA
  GGACAGACGGAAGGCAGAAGCCGAGAGAGCTCAGCGGATGGCCCAAGAAAGGCAGGAGCAGGAGGAGGCT
  CGAAGGAAGCTGGAAGAGCAAGCCAGAGCCAAGAAGCAGACACCCCCTGCATCCCCTAGTCCTCAGCCAG
  CTGAAGACAGACCACCATCCAGCCCCATCTATGAGGATGCAGCTCCGTTGAAGGCCGAGCCGAGCTACGG
  AAGTAGTGAGCCTGAGCCTGAGTACAGCACTGAGGCTGCAGGCCTTCCTGAGGCCAGCAACCAGCAAGGT
  CTGGCCTACACATCAGAGCCTGTTTACGAGACCACAGAGGTTCCTGGCCACTACCAAGCAGAGGATGACA
  CCTACGATGGGTATGAGAGTGACCTGGGCATCACAGCCATCGCCCTGTATGACTACCAGGCTGCTGGCGA
  TGATGAAATCTCCTTTGACCCTGATGATGTCATCACCAACATAGAAATGATTGACGATGGCTGGTGGCGT
  GGGGTGTGCAAGGGCAGATATGGGCTCTTCCCGGCCAACTATGTGGAGCTGCGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR203142 representing NM_021868
  Red=Cloning site Green=Tags(s)
  
  MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLK
  EKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFE
  YQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSA
  VGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYAKGFGGKYGVQKDRM
  DKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAQERQEQEEA
  RRKLEEQARAKKQTPPASPSPQPAEDRPPSSPIYEDAAPLKAEPSYGSSEPEPEYSTEAAGLPEASNQQG
  LAYTSEPVYETTEVPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDVITNIEMIDDGWWR
  GVCKGRYGLFPANYVELRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021868
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021868.2, NP_068640.2
• RefSeq Size: 2951 bp
• RefSeq ORF: 1530 bp
• Locus ID: 60465
• UniProt ID: Q66HL2
• Cytogenetics: 1q41-q42
• MW: 56.9 kDa
• Gene Summary: component of clathrin-coated pits; involved in receptor-mediated endocytosis [RGD, Feb 2006]