Cactin (NM_001081447) Rat Tagged ORF Clone

CAT#: RR202971

Cactin (Myc-DDK-tagged ORF) - Rat similar to R31449_3 (RGD1563634), (10 ug)

Product Images

Circular map for RR202971

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cactin
• Synonyms: RGD1563634
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202971 representing NM_001081447
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGGCGCGGGCCGCCAAATGGGTTCGCACTCTCGCTCGCGCTCACGATCTGCGGGTCGTAGGAGTC
  GGAAGCAGCGGAGTAGGAGTAGAAGCTGCAGCAGGAGCCGGAGCCGAAGCCGGAGCCGGAGCCGGAGCCG
  GAGCCATGGGGGGAGCGGCCGAAGGCGACGGGAACATGAGCGGCGGCGCGAACGCAAACGGCGCAGTCGA
  GGGCGCAGGTCAGATTCGGAAGGAAACCGTGTGCAGAAGTCTAGGAGGCGAAGCCGGAGCCTTCGGCCAC
  CCCGATGGCACTCACAGGACCGGTCCTCACACTCTGACTCGGGTGAGGAGCAAGCACAGGGCTCCCGAGC
  CCGGAAGGGTCACAGGCATTCGTGGTCCCCAGGGTCATCAGCATCTAGCTCTGACTCCCCAAGACGCTCC
  CGGAGTCCTGGAGTAGCTGCCCTGGCCGCGAGTCAGCAGCAGAGCCTTCAGGAACGGCTGCGGCTTCGTG
  AGGAGCGAAAGCAGCAAGAAGAGCTGCTCAAGGCCTTCGAGACACCCGAGGAGAAGCGTGCCCGGCGACT
  GGCCAAGAAAGAAGCTAAGGAGCGCAAGAAGCGTGAGAAGATGGGCTGGGGTGAGGAGTACATGGGCTAC
  ACCAACACCGACAACCCCTTTGGAGACAACAACCTGCTGGGCACTTTTATCTGGAACAAAGCCTTGGAGA
  AAAAAGGCATCGGTCACCTGGAGGAAAAGGAGCTGAAGGAACGTAACAAGAGGATCCAGGAGGAGAACCG
  GCTAGAGCTGCAAAAGGTAAAACAGCTGCGGCTGGAGCGGGAACGGGAGAAGGCCATGCGGGAGCAGGAA
  CTGGAACTGTTGCAACGGGAGAAGGAGGCAGAGCACTTTAAGACCTGGGAGGAGCAGGAGGACAGCTTCC
  ACTTGCGGCAGGCCAAGCTTCGTTCCAAGATCCGAATCCGAGACGGGCGGGCCAAGCCCATTGACTTGCT
  GGCCAAGTACATCAGTGCAGAGGACGATGACCTGGCAGTGGAGATGCATGAACCTTACACCTTCCTCAAC
  GGCCTCACGGTGGCAGACATGGAGGACCTGCTAGAGGACATCCAGGTGTACATGGAGCTGGAGCAGGGCA
  AGAACGTGGACTTCTGGCGAGACATGACAACCATCACAGAGGATGAGATTGCCAAGCTCCGCAAGCTGGA
  GGCCTCGGGCAAGGGCCCAGGTGAGCGCCGTGAGGGGGTCAACGCCTCGGTCAGCTCTGACGTGCAGTCC
  GTCTTCAAGGGAAAGACGTACAACCAGCTGCAGGTCATCTTCCAGGGCATCGAAGGCAAGATCCGTGCCG
  GCGGCCCCAACCTCGACATGGGCTACTGGGAGAGCCTGCTGCAGCAGTTGCGCGCGCACATGGCCAGGGC
  CAGGCTCCGTGAGCGCCACCAAGATGTCCTTAGACAGAAGCTGTTCAAACTGAAGCAGGAGCAGGGTGTG
  GAGAGTGAGCCGCTGTTTCCCATCCTCAAGTCAGAGCCCACAGCCACTCACAGCCCTGAACCTGAGGAAC
  GGCCTCCCAGCCCTGGGACTTCAGTGGACCCCGTGGAACCCGAGGAGACCACAGCAGCAGGCGAGGCAGA
  GGGAGAGGCAGATGGCGAGGCAGTGCTGATGGAGGAGGACCTGATCCAGCAGAGCCTGGCAGACTATGAC
  GCTGGCCGCTACAGCCCACGACTGCTCACCGCGCACGAGTTGCCTTTGGACGCGCATGTGCTGGAGCCGC
  ATGAGGACCTGCAGCGCCTGCAGCTGTCTCGCCAGCAGCTTCAGGCTACAGGTGACGCGAGCGAGAGTGC
  TGAGGACATCTTCTTCCGGCGTGCGAGGGAGGGCATGGGGCAGGATGAGGCACAGTTCAGCGTGGAGATG
  CCACTGGGTGGCCGCGCCTACCTGTGGGCCGACAAGTATCGGCCGCGGAAGCCGCGATTCTTTAACCGTG
  TGCACACAGGCTTTGAATGGAACAAATATAACCAGACACATTATGACTTTGACAACCCTCCACCCAAGAT
  CGTGCAGGGCTACAAATTTAACATCTTCTACCCGGACCTCATCCGCAAACGTGCCACACCAGAGTACTTC
  CTGGAGGCCTGCGCTGACAACCGGGACTTTGCCATCCTGCGTTTCCACGCAGGCCCGCCCTACGAGGACA
  TCGCCTTCAAGATCGTCAGTCGAGAATGGGAGTACTCGCACCGCCATGGCTTCCGCTGCCAGTTTGCCAA
  TGGAATCTTCCAGCTCTGGTTCCACTTCAAGCGCTACCGCTACCGCCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202971 representing NM_001081447
  Red=Cloning site Green=Tags(s)
  
  MRGAGRQMGSHSRSRSRSAGRRSRKQRSRSRSCSRSRSRSRSRSRSRSHGGSGRRRREHERRRERKRRSR
  GRRSDSEGNRVQKSRRRSRSLRPPRWHSQDRSSHSDSGEEQAQGSRARKGHRHSWSPGSSASSSDSPRRS
  RSPGVAALAASQQQSLQERLRLREERKQQEELLKAFETPEEKRARRLAKKEAKERKKREKMGWGEEYMGY
  TNTDNPFGDNNLLGTFIWNKALEKKGIGHLEEKELKERNKRIQEENRLELQKVKQLRLEREREKAMREQE
  LELLQREKEAEHFKTWEEQEDSFHLRQAKLRSKIRIRDGRAKPIDLLAKYISAEDDDLAVEMHEPYTFLN
  GLTVADMEDLLEDIQVYMELEQGKNVDFWRDMTTITEDEIAKLRKLEASGKGPGERREGVNASVSSDVQS
  VFKGKTYNQLQVIFQGIEGKIRAGGPNLDMGYWESLLQQLRAHMARARLRERHQDVLRQKLFKLKQEQGV
  ESEPLFPILKSEPTATHSPEPEERPPSPGTSVDPVEPEETTAAGEAEGEADGEAVLMEEDLIQQSLADYD
  AGRYSPRLLTAHELPLDAHVLEPHEDLQRLQLSRQQLQATGDASESAEDIFFRRAREGMGQDEAQFSVEM
  PLGGRAYLWADKYRPRKPRFFNRVHTGFEWNKYNQTHYDFDNPPPKIVQGYKFNIFYPDLIRKRATPEYF
  LEACADNRDFAILRFHAGPPYEDIAFKIVSREWEYSHRHGFRCQFANGIFQLWFHFKRYRYRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081447
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081447.1, NP_001074916.1
• RefSeq Size: 2595 bp
• RefSeq ORF: 2292 bp
• Locus ID: 500790
• Cytogenetics: 7q11
• MW: 89.3 kDa