Cd44 (NM_012924) Rat Tagged ORF Clone

CAT#: RR202727

Cd44 (Myc-DDK-tagged ORF) - Rat Cd44 molecule (Cd44), (10 ug)

Product Images

Circular map for RR202727

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd44
• Synonyms: CD44A; METAA; RHAMM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202727 representing NM_012924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAGGTTTGGTGGCACACAGCTTGGGGACTACTTTGCCTCTTACAGTTGAGCCTGGCACAGCAGC
  AGATCGATTTGAATATAACCTGCCGTTACGCAGGTGTATTCCATGTGGAGAAAAATGGCCGCTACAGTAT
  CTCCAGGACTGAAGCAGCTGACCTCTGCGAGGCTTTCAACACCACCTTGCCCACCATGGCTCAGATGGAG
  TTAGCCCTGAGAAAGGGGTTTGAAACATGCAGGTATGGGTTCATAGAAGGACACGTGGTAATCCCGAGGA
  TCCACCCCAACGCTATCTGTGCAGCCAACAACACAGGAGTGTATATCCTCCTCGCATCCAACACCTCCCA
  CTATGACACATATTGCTTCAATGCCTCAGCTCCTCTTGAAGAAGACTGTACATCAGTCACAGACCTACCC
  AATTCCTTCGATGGACCAGTTACCATAACTATTGTCAACCGTGATGGCACCCGCTACAGCAAGAAGGGCG
  AGTATAGAACACACCAAGAAGACATCGATGCCTCAAACATTATAGATGAGGATGTCAGCAGTGGATCCAC
  CATTGAGAAGAGCACCCCAGAAGGCTACATTTTGCACACCGACCTTCCCACTTCACAGCCTACTGGAGAC
  CGGGATGACGCCTTCTTTATTGGGAGCACCCTGGCCACCATTGCCTCAACTGTGTACTCAAAAAGCCATG
  CAACAGCTCAGAAACAAAATAACTGGATATGGTCTTGGTTTGGTAATTCACAATCCACAACTCAGACTCA
  GGATAGCCCCACAACAACAGCAACTACAGCCTTGATGACTACCCCTGAAACACCACCCAAGAGGCAAGAA
  GCCCAGAACTGGTTTTCATGGTTTTTTCAACCATCAGAGTCCAAGAGTCATCTTCATACAACAACGAAGA
  TGCCTGGTACGGAGTCAAATACCAACCCAACAGGCTGGAAGCCAAATGAGGAAAATGAAGATGAAACAGA
  CAAATACCCCAATTTTTCTGGATCAGGCATTGATGATGATGAAGATTTTATCTCCAGCACCATTGCAACT
  ACTCCATGGGTTTCTGCCCACACAAAACAGAACCAGGAACGGACCCAGTGGAACCCAATCCATTCAAACC
  CAGAAGTACTACTTCAGACAACCACCAGGATGACTGATATAGACAGAAACAGCACCAGTGCTCATGGAGA
  AAACTGGACCCAGGAACCACAGCCTCCTTTCAATAACCATGAGTATCAGGATGAAGAGGAGACCCCACAT
  GCTACAAGCACAACCTGGGCAGATCCTAATAGCACAACAGAAGAAGCAGCTACCCAGAAGGAGAAGTGGT
  TTGAGAATGAATGGCAGGGGAAGAACCCACCCACCCCAAGTGAAGACTCCCATGTGACAGAAGGGACAAC
  TGCCTCAGCCCACAACAACCATCCAAGTCAAAGAATGACAACACAGAGTCAAGAGGATGTTTCATGGACC
  GATTTCTTCGACCCAATCTCACATCCAATGGGACAAGGTCATCAAACAGAAAGCAAGGATACAGGCTCCA
  GTCATAGTACAACCCTTCAGCCTACTGCGGCTCCAAATACCCATTTGGTGGAAGACTTGAACAGGACAGG
  ACCACTTTCAGTGACAACTCCACAGAGTCATTCTCAGAACTTCTCTACATTACCTGGAGAGCTGGAAGAA
  GGCGAAGACCATCCAACAACTTCTGTTCTGCCATCTAGCACTAAGAGTGGTCGAAGAAGAGGTGGAAGTC
  TTCCCAGAGATACAACTACTTCACTGGAAGGCTACACCCCTCAATATCCAGACACAATGGAAAACGGGAC
  TCTCTTCCCAGTGACCCCTGCTAAGACTGAGGTCTTTGGAGAAACTGAAGGGACTGTTGCTACTGACTCC
  AACTTTAATGTGGATGGCTCCTTACCAGGTGATGGAGACTCATCCATGGACCCCAGGGGTGGTTTCGACA
  CTGTGACTCATGGATCCGAATTAGCTGGACACTCAAGTGGGAATCAAGACAGTGGAGTGACCACAACTTC
  TGGTCCTGCGAGGAGACCTCAGATTCCAGAGTGGCTTATCATCTTGGCATCCCTCCTGGCGCTGGCTCTG
  ATTCTTGCCGTCTGCATTGCTGTCAACAGTAGGAGAAGGTGTGGGCAGAAGAAGAAGCTGGTGATCAACA
  GTGGCAATGGAACAGTGGAAGACAGGAAACCAAGTGAACTCAACGGGGAGGCCAGCAAGTCTCAGGAAAT
  GGTGCATTTGGTGAACAAGGAACCAACAGAGACTCCGGACCAGTTTATGACAGCTGATGAGACCCGGAAT
  CTGCAGAGTGTGGATATGAAGATTGGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202727 representing NM_012924
  Red=Cloning site Green=Tags(s)
  
  MDKVWWHTAWGLLCLLQLSLAQQQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCEAFNTTLPTMAQME
  LALRKGFETCRYGFIEGHVVIPRIHPNAICAANNTGVYILLASNTSHYDTYCFNASAPLEEDCTSVTDLP
  NSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDEDVSSGSTIEKSTPEGYILHTDLPTSQPTGD
  RDDAFFIGSTLATIASTVYSKSHATAQKQNNWIWSWFGNSQSTTQTQDSPTTTATTALMTTPETPPKRQE
  AQNWFSWFFQPSESKSHLHTTTKMPGTESNTNPTGWKPNEENEDETDKYPNFSGSGIDDDEDFISSTIAT
  TPWVSAHTKQNQERTQWNPIHSNPEVLLQTTTRMTDIDRNSTSAHGENWTQEPQPPFNNHEYQDEEETPH
  ATSTTWADPNSTTEEAATQKEKWFENEWQGKNPPTPSEDSHVTEGTTASAHNNHPSQRMTTQSQEDVSWT
  DFFDPISHPMGQGHQTESKDTGSSHSTTLQPTAAPNTHLVEDLNRTGPLSVTTPQSHSQNFSTLPGELEE
  GEDHPTTSVLPSSTKSGRRRGGSLPRDTTTSLEGYTPQYPDTMENGTLFPVTPAKTEVFGETEGTVATDS
  NFNVDGSLPGDGDSSMDPRGGFDTVTHGSELAGHSSGNQDSGVTTTSGPARRPQIPEWLIILASLLALAL
  ILAVCIAVNSRRRCGQKKKLVINSGNGTVEDRKPSELNGEASKSQEMVHLVNKEPTETPDQFMTADETRN
  LQSVDMKIGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012924
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012924.2, NP_037056.2
• RefSeq Size: 4038 bp
• RefSeq ORF: 2343 bp
• Locus ID: 25406
• Cytogenetics: 3q32
• MW: 85.9 kDa
• Gene Summary: adhesion molecule involved in migration, cell fusion and resportion in osteoclasts that also plays a role in cellular metastasis [RGD, Feb 2006]