Cd248 (NM_001106325) Rat Tagged ORF Clone

CAT#: RR202433

Cd248 (Myc-DDK-tagged ORF) - Rat CD248 molecule, endosialin (Cd248), (10 ug)

Product Images

Circular map for RR202433

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd248
• Synonyms: Cd164l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202433 representing NM_001106325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGCGCCTGCTGCTGGCCTGGGCGGCCGCGGTGCCCGCACTGGGCCAGGCCCCCTGGACGCCGG
  AGCCTAGAGCCGCCTGCGGCCCCAGCAGCTGCTACGCTCTCTTTCCCCGGCGCCGCACATTCCTGGAGGC
  TTGGCGGTCGTGCCGCGAATTGGGGGGCAACCTGGCCACACCGAGGACCCCGGAGGAGGCCCGACGTGTG
  GACAGCCTGGTGGGCGTCGGACCCGCCAACGGGCTGCTATGGATTGGGTTGCAGCGGCAGGCTCGGCAAT
  GCCAGCCACAGCGCCCACTGCGGGGCTTCATATGGACCACGGGAGACCAGGACACCGCCTTCACTAACTG
  GGCCCAGCCGGCTACGGAAGGACCCTGCCCGGCCCAGCGCTGTGCTGCCCTTGAGGCCAGCGGAGAACAT
  CGCTGGCTCGAAGGCTCGTGCACACTGGCTGTCGATGGCTACCTCTGCCAGTTTGGTTTTGAGGGTGCCT
  GTCCTGCCTTGCCGCTTGAGGTGGGCCAAGCCGGTCCAGCTATCTACACCACACCCTTCAACCTGGTTTC
  CAGTGAGTTCGAATGGCTACCCTTTGGCTCCGTGGCAGCTGTGCAGTGCCAAGCTGGCAGGGGAACGTCT
  CTGTTGTGTGTGAAACAACCTTCAGGTGGCGTTGGCTGGTCCCAGACTGGCCCACTGTGTCCAGGGACTG
  GCTGTGGTCCTGACAATGGGGGTTGCGAACATGAATGTGTGGAAGAGTTGGATGGCGGTATGTCCTGCCG
  CTGCAGTGAAGGCTTCCGTCTAGCAGCAGATGGGCACAGTTGTGAAGACCCTTGTGCCCAGGCCCCCTGT
  GAGCAGCAGTGTGAGCCTGGTGGGCCACAAGGCTACAGCTGCCACTGTCGCCTAGGCTTCCGGCCAGCTG
  AGGATGAGCCACACCGCTGCGTGGACACGGATGAGTGCCAGATTGCTGGTGTGTGCCAGCAGATGTGTGT
  CAACTATGTTGGTGGCTTTGAGTGTTACTGCAGGGAGGGTCATGAGCTTGAGGCAGATGGTATCAGTTGT
  AGCCCTGCAGGAGCTATGGGTGCCCAGGCTTCCCAGGATCTTAGAGACGAGTTGCTGGATGATGGAGAAG
  AAGGGGAGGATGAAGAGGAGCCCTGGGAGGACTTCGATGGCACCTGGACAGAGGAGCAGGGGACCCTATG
  GATGGCACCTACACATCCGCCTGACTTTGGCCTGCCCTATAGGCCCAACTTCCCACAGGATGGAGAGCCT
  CAGAGATTGCACCTGGAGCCTACCTGGCCACCCCCACTTAGCGCCCCCAGGGGCCCCTACCACTCCTCAG
  TGGTGTCTGCCACACGGCCCATGGTAATCTCTGCCACTCGACCCACACAACCTTCTGCCCGAAAGACCTC
  TGTTATTTCAGCCACACACCTACCCCTTAACCCTGTCCACCCACCTGCCCTAGCCCCTACCACACCTCCA
  GCCGTGCTCCCTGAGCACCAGATCCCCAAAATCAAGGCCAGTTATCCAGACTTGCCTTTTGGCCACAAGC
  CTGGGATAACCTCAGCCACTCACCCAGCACAGCCTCCTCCTCACCAGCCCCCCATCATCTCAACGAAATA
  TCCCCAAGTCTTCCCTCCCCAGCAGGCCCCTATGTCTCCAGACACCCACACTATCACTAATTTGCCTCTA
  ATCCCATCTCACCTTGACCCTGGGGATACCACTTCCCAAGCCGGTCACCATCCTTTGCTCCCAGATGTTC
  CAGGTATCAGAACCCAGGCTCCCCAGGTTTCTGTCTCAGCTCTCCAGCCCTCTCTGCCTACCAACTCCAG
  GTCTTCTGTCCATGAACCCCCTGTGCCTACTGCCAACCAGCCCCCAGCCTTCCCTTCTCCCCTGCCCCCT
  CAGAGCCCCATTAACCAGACCTCATCTATCAGCCCTACACACTCCTATTCCAGAGCCCCTCAGGTCCCAA
  GGGAAGGAGCTCCCAGTCCCAAATCAGTGCCAAGGCTGCACTCAGTGGCCCCCACAGCAGCTCCAACAGC
  CCTGGCAGAGTTGGGTCTTGCAGGCCAAAGCCAGAGAGATGACCGATGGCTGCTGGTGGCACTCTTGGTA
  CCAACGTGTGTCTTCTTGGTGGTCCTGCTCGCATTGGGCATTGTGTACTGCACTCGCTGTGGCTCCCATA
  CGCCCAACAAGCGTATCACTGACTGCTATCGCTGGGTCACGCATGCTGGGAACAAGAGCTCAACAGAACC
  CATGCCCCCCAGAGGCAGCCTTACAGGGGTACAGACCTGTAGAACCAGTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202433 representing NM_001106325
  Red=Cloning site Green=Tags(s)
  
  MLLRLLLAWAAAVPALGQAPWTPEPRAACGPSSCYALFPRRRTFLEAWRSCRELGGNLATPRTPEEARRV
  DSLVGVGPANGLLWIGLQRQARQCQPQRPLRGFIWTTGDQDTAFTNWAQPATEGPCPAQRCAALEASGEH
  RWLEGSCTLAVDGYLCQFGFEGACPALPLEVGQAGPAIYTTPFNLVSSEFEWLPFGSVAAVQCQAGRGTS
  LLCVKQPSGGVGWSQTGPLCPGTGCGPDNGGCEHECVEELDGGMSCRCSEGFRLAADGHSCEDPCAQAPC
  EQQCEPGGPQGYSCHCRLGFRPAEDEPHRCVDTDECQIAGVCQQMCVNYVGGFECYCREGHELEADGISC
  SPAGAMGAQASQDLRDELLDDGEEGEDEEEPWEDFDGTWTEEQGTLWMAPTHPPDFGLPYRPNFPQDGEP
  QRLHLEPTWPPPLSAPRGPYHSSVVSATRPMVISATRPTQPSARKTSVISATHLPLNPVHPPALAPTTPP
  AVLPEHQIPKIKASYPDLPFGHKPGITSATHPAQPPPHQPPIISTKYPQVFPPQQAPMSPDTHTITNLPL
  IPSHLDPGDTTSQAGHHPLLPDVPGIRTQAPQVSVSALQPSLPTNSRSSVHEPPVPTANQPPAFPSPLPP
  QSPINQTSSISPTHSYSRAPQVPREGAPSPKSVPRLHSVAPTAAPTALAELGLAGQSQRDDRWLLVALLV
  PTCVFLVVLLALGIVYCTRCGSHTPNKRITDCYRWVTHAGNKSSTEPMPPRGSLTGVQTCRTSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001106325
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001106325.1, NP_001099795.1
• RefSeq Size: 2565 bp
• RefSeq ORF: 2295 bp
• Locus ID: 293669
• Cytogenetics: 1q43
• MW: 81.8 kDa