Abcc5 (NM_053924) Rat Tagged ORF Clone

CAT#: RR202372

Abcc5 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family C (CFTR/MRP), member 5 (Abcc5), (10 ug)

Product Images

Circular map for RR202372

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abcc5
• Synonyms: Abcc5a; Mrp5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202372 representing NM_053924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGATATTGACATGGGAAAAGAATACATCATCCCCAGTCCCGGATACAGAAGCGTCAGGGACAGAA
  GCACTATCCCGGGACAACACGGCGATCGTGAAGAACCCAGGTTCCGGAGAACAAGATCATTGGAATGCCA
  AGATGCCCTAGAAACAGCAGCCCGAGTTGAGGGTCTTTCCCTGGACATCTCTGTGCATTCTCATCTCCAA
  ATTCTGGACGAGGAACATACGAAGGGGAAATACCACCATGGTTTAAGCGCCCTGAAGCCCTTCCGGACCA
  CCACCAAGCACCAGCACCCAGTGGACAATGCTGGCCTTTTCTCCTACATGACCTTTTCATGGCTCTCTCC
  TCTGGCCCAAGTGGTTCACAAGAAGGGGGAGCTGTTAATGGAGGATGTGTGGCCTTTGTCCAAGTATGAG
  TCTTCTGATGTGAACTGCAGAAGACTAGAGAGACTGTGGCAAGAAGAGCTGAATGAAGTCGGGCCAGACG
  CCGCCTCCCTGCGAAGGGTTGTGTGGATCTTTTGCCGCACCAGGCTCATCCTGTCCATCGTGTGCCTGAT
  GATCACGCAGTTGGCTGGCTTCAGTGGACCAGCCTTCGTGGTGAAGCACCTCTTGGAGTACACCCAGGCA
  ACAGAGTCTAACCTGCAGTACAGCTTGCTGTTGGTACTAGGCCTCCTCCTGACAGAGGTTGTACGCTCCT
  GGTCACTCGCACTGACTTGGGCATTGAATTATCGCACTGGTGTCCGGCTGCGGGGGGCTGTCCTGACCAT
  GGCATTCAAGAAGATCCTGAAGCTAAAGAACATTAAGGAAAAGTCCCTGGGGGAGCTTATCAATATCTGC
  TCCAACGATGGGCAGAGGATGTTTGAAGCAGCCGCTGTGGGCAGCTTGCTGGCCGGAGGACCCGTTGTCG
  CCATCTTGGGCATGATTTATAATGTAATCATTCTAGGACCAACGGGCTTCCTGGGATCGGCTGTCTTTAT
  CCTCTTTTATCCGGCAATGATGTTCGTGTCACGGCTAACCGCGTATTTCAGGAGAAAGTGTGTAGCCGCC
  ACAGACGACCGTGTCCAGAAGATGAATGAAGTTCTTACCTACATTAAATTTATTAAAATGTATGCCTGGG
  TCAAAGCGTTTTCTCAGTGTGTTCAAAAAATCCGAGAGGAGGAGCGTCGGATCCTGGAGAAGGCCGGGTA
  CTTTCAGAGCATCACCGTTGGAGTGGCTCCCATTGTCGTAGTGATCGCCAGCGTGGTGACGTTCTCCGTC
  CACATGACCCTGGGCTTCGACCTGACAGCGGCGCAGGCCTTCACAGTGGTGACTGTCTTCAATTCCATGA
  CTTTTGCTTTAAAAGTAACACCATTCTCAGTGAAGTCCCTCTCTGAAGCATCCGTTGCTGTTGACAGATT
  TAAGAGTTTGTTTCTAATGGAAGAGGTTCACATGATAAAGAACAAACCGGCCAGTCCTCACATCAAGATA
  GAGATGAAAAATGCCACCTTGGCATGGGACTCCTCCCACTCCAGTACACAGAGCTCGCCCAAGCTGACCC
  CCAAAGTGAAAAAAGACAAGAGGGCTCCCAAGGGCAAGAAAGAGAAGTCGAGGCAGCTGCAACATACTGA
  GCACCAGGCGGTGCTGGCAGAGCAGAAAGGACACCTCCTCCTGGACAGTGATGAGCGGCCCAGTCCGGAA
  GAGGAAGAAGGCAAGCAGATCCACGCAGGGAGCATGCGTCTGCAGAGGACACTCTACAACATTGACTTAG
  AGATTGAAGAGGGCAAACTGGTTGGAATCTGTGGCAGTGTGGGAAGTGGAAAAACCTCTCTGATTTCCGC
  CATTTTAGGCCAGATGACGCTTTTGGAGGGCAGCATTGCCGTCAGTGGGACCTTTGCTTACGTGGCCCAA
  CAGGCCTGGATTCTCAACGCCACGCTGAGAGACAACATTCTCTTTGGGAAGGAATTTGATGAAGAGAGAT
  ACAACTCAGTGCTGAACAGTTGCTGCCTGCGGCCTGACTTGGCCATTCTCCCCAACAGCGACCTGACTGA
  GATTGGAGAGCGAGGAGCCAACCTGAGTGGTGGGCAGCGCCAGCGAATCAGCCTTGCTCGAGCCCTGTAC
  AGTGACAGAAGCATCTATATCTTGGATGATCCCCTCAGTGCCTTAGATGCCCATGTGGGCAACCACATCT
  TCAACAGTGCTATCCGGAAGCGTCTCAAGTCTAAGACGGTCCTGTTTGTCACACACCAGTTACAGTACCT
  GGTCGATTGTGATGAGGTGATCTTCATGAAAGAAGGCTGTATCACAGAGAGAGGTACCCACGAGGAGCTG
  ATGAACTTAAATGGAGACTACGCTACCATTTTTAATAACCTGTTGCTGGGAGAGACACCCCCGGTCGAGA
  TTAATTCAAAAAAGGAAGCTAGTGGTTCACAAAAATCACAAGACAAGGGCCCTAAGCCAGGGTCAGTGAA
  GAAGGAGAAGGCAGTGAAGTCGGAGGAAGGGCAGCTCGTGCAGGTGGAGGAGAAAGGGCAGGGCTCCGTG
  CCTTGGTCAGTTTACTGGGTCTACATCCAGGCTGCAGGGGGTCCCTTGGCTTTCCTGGTCATCATGGTCC
  TCTTCATGCTCAATGTGGGCAGCACTGCCTTCAGCACCTGGTGGCTAAGCTACTGGATCAAGCAAGGAAG
  CGGGAACAGCACGGTGTTTGAAGGGAACAGAAGCTCTGTGAGTGACAGCATGAGGGACAACCCCTTCCTG
  CAGTACTATGCCAGCATCTACGCTCTCTCCATGGCAGTCATGCTGATCCTGAAGGCCATCCGAGGAGTTG
  TCTTTGTCAAGGGTACACTGAGAGCCTCCTCCCGGCTCCATGATGAGCTCTTCCGGAGGATCCTGAGAAG
  CCCCATGAAGTTTTTTGATACTACCCCAACAGGAAGGATTCTCAACAGGTTTTCCAAAGACATGGATGAA
  GTGGACGTGCGTCTGCCATTCCAGGCTGAGATGTTTATCCAGAATGTGATCCTGGTGTTCTTCTGTGTTG
  GAATGATCGCAGGGGTCTTCCCATGGTTCCTTGTGGCAGTGGGGCCTCTCCTCATCCTCTTCTCAGTTCT
  ACACATTGTCTCCAGGGTCCTGATTCGGGAGCTGAAGCGCTTGGACAATATCACGCAATCTCCTTTCCTC
  TCCCATATCACGTCCAGCATTCAGGGCCTCGCCACCATCCATGCCTATAACAAAAGGCAGGAGTTTCTAC
  ACAGGTATCAGGAGCTTCTGGATGACAACCAAGCCCCCTTCTTCTTGTTCACCTGTGCAATGAGGTGGCT
  GGCAGTGCGGCTGGACCTCATCAGCATCGCCCTGATCACCACCACGGGGCTCATGATTGTTCTCATGCAC
  GGGCAGATCCCTTCAGCGTACGCGGGCCTCGCCATCTCCTACGCTGTGCAGTTAACCGGACTCTTCCAGT
  TTACCGTTAGACTGGCGTCGGAGACAGAAGCACGGTTCACCTCAGTGGAGAGGATCAACCACTACATAAA
  GACTCTCTCTTTGGAAGCACCTGCCAGAATCAAGAACAAGGCTCCTCCCCATGACTGGCCCCAGGAGGGA
  GAAATAACCTTTGAGAATGCGGAAATGAGATACCGGGAAAATCTCCCTCTGGTCCTTAAGAAAGTGTCCT
  TCACCATCAAGCCCAAGGAAAAGATAGGCATTGTGGGACGAACAGGGTCAGGGAAGTCCTCTCTAGGGAT
  GGCCCTCTTCCGCCTGGTGGAGTTATCTGGAGGCTGCATCAAGATCGATGGAGTAAGAATCAGTGACATT
  GGCCTGGCCGACCTCCGAAGCAAACTGACCATCATCCCTCAGGAGCCAGTGCTGTTCAGCGGCACAGTCA
  GATCAAACCTGGACCCTTTCAACCAGTACACAGAAGAGCAGATCTGGGATGCCCTAGAGAGAACACACAT
  GAAGGAATGTATTGCCCAGCTACCTCTGAAACTGGAATCTGAAGTAATGGAGAACGGGGACAACTTCTCT
  GTTGGGGAACGGCAGTTGTTATGCATAGCAAGGGCCCTGCTACGTCACTGTAAGATTCTGATTTTAGATG
  AAGCTACAGCCGCTATGGACACAGAGACAGACTTACTGATCCAAGAGACCATCCGGGAAGCATTTGCTGA
  CTGCACCATGCTGACCATTGCCCATCGCCTGCACACAGTTCTGGGCTCTGATAGGATCATGGTGCTGGCC
  CAGGGACAGGTGGTGGAGTTTGACACCCCGTCGGTCCTTCTGTCCAACGACAGCTCAAGATTCTATGCCA
  TGTGTGCTGCTGCAGAGAACAAGGTGGCTGTCAAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202372 representing NM_053924
  Red=Cloning site Green=Tags(s)
  
  MKDIDMGKEYIIPSPGYRSVRDRSTIPGQHGDREEPRFRRTRSLECQDALETAARVEGLSLDISVHSHLQ
  ILDEEHTKGKYHHGLSALKPFRTTTKHQHPVDNAGLFSYMTFSWLSPLAQVVHKKGELLMEDVWPLSKYE
  SSDVNCRRLERLWQEELNEVGPDAASLRRVVWIFCRTRLILSIVCLMITQLAGFSGPAFVVKHLLEYTQA
  TESNLQYSLLLVLGLLLTEVVRSWSLALTWALNYRTGVRLRGAVLTMAFKKILKLKNIKEKSLGELINIC
  SNDGQRMFEAAAVGSLLAGGPVVAILGMIYNVIILGPTGFLGSAVFILFYPAMMFVSRLTAYFRRKCVAA
  TDDRVQKMNEVLTYIKFIKMYAWVKAFSQCVQKIREEERRILEKAGYFQSITVGVAPIVVVIASVVTFSV
  HMTLGFDLTAAQAFTVVTVFNSMTFALKVTPFSVKSLSEASVAVDRFKSLFLMEEVHMIKNKPASPHIKI
  EMKNATLAWDSSHSSTQSSPKLTPKVKKDKRAPKGKKEKSRQLQHTEHQAVLAEQKGHLLLDSDERPSPE
  EEEGKQIHAGSMRLQRTLYNIDLEIEEGKLVGICGSVGSGKTSLISAILGQMTLLEGSIAVSGTFAYVAQ
  QAWILNATLRDNILFGKEFDEERYNSVLNSCCLRPDLAILPNSDLTEIGERGANLSGGQRQRISLARALY
  SDRSIYILDDPLSALDAHVGNHIFNSAIRKRLKSKTVLFVTHQLQYLVDCDEVIFMKEGCITERGTHEEL
  MNLNGDYATIFNNLLLGETPPVEINSKKEASGSQKSQDKGPKPGSVKKEKAVKSEEGQLVQVEEKGQGSV
  PWSVYWVYIQAAGGPLAFLVIMVLFMLNVGSTAFSTWWLSYWIKQGSGNSTVFEGNRSSVSDSMRDNPFL
  QYYASIYALSMAVMLILKAIRGVVFVKGTLRASSRLHDELFRRILRSPMKFFDTTPTGRILNRFSKDMDE
  VDVRLPFQAEMFIQNVILVFFCVGMIAGVFPWFLVAVGPLLILFSVLHIVSRVLIRELKRLDNITQSPFL
  SHITSSIQGLATIHAYNKRQEFLHRYQELLDDNQAPFFLFTCAMRWLAVRLDLISIALITTTGLMIVLMH
  GQIPSAYAGLAISYAVQLTGLFQFTVRLASETEARFTSVERINHYIKTLSLEAPARIKNKAPPHDWPQEG
  EITFENAEMRYRENLPLVLKKVSFTIKPKEKIGIVGRTGSGKSSLGMALFRLVELSGGCIKIDGVRISDI
  GLADLRSKLTIIPQEPVLFSGTVRSNLDPFNQYTEEQIWDALERTHMKECIAQLPLKLESEVMENGDNFS
  VGERQLLCIARALLRHCKILILDEATAAMDTETDLLIQETIREAFADCTMLTIAHRLHTVLGSDRIMVLA
  QGQVVEFDTPSVLLSNDSSRFYAMCAAAENKVAVKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053924
• ORF Size: 4308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053924.1, NP_446376.1
• RefSeq Size: 5744 bp
• RefSeq ORF: 4311 bp
• Locus ID: 116721
• UniProt ID: Q9QYM0
• Cytogenetics: 11q23
• MW: 160.9 kDa
• Gene Summary: ATP-binding cassette (ABC) multiple drug resistance protein that mediates efflux cGMP during nitric oxide-induced regulation of smooth muscle [RGD, Feb 2006]