Kcnma1 (NM_031828) Rat Tagged ORF Clone

CAT#: RR202293

Kcnma1 (Myc-DDK-tagged ORF) - Rat potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), (10 ug)

Product Images

Circular map for RR202293

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnma1
• Synonyms: BKCa; cbv1; Kcnma; KCNMA1b; KCNMA1c; Slo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202293 representing NM_031828
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCG
  GAGGCGAGACGGCTCTTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCTTC
  TTCCTCCTCCTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTC
  ATCATCCCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCT
  CCTCCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACTCTCAAGTACCTGTGGAC
  CGTTTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGAT
  GGTACTCTCAAGCCAGTGGATGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGA
  AGGACTGGGCAGGGGTGATGATATCTGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGC
  TCTCAGCATTGGTGCCCTTGTAATATACTTCATAGACTCATCAAACCCAATAGAATCCTGCCAGAATTTC
  TACAAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTTCTCTACTTTGGCTTGCGGT
  TTATTGCAGCCAATGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCTGTAGTAGATTTCTTCACAGTCCC
  TCCCGTGTTTGTGTCTGTATACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTG
  ATTCAGTTTTCAGAAATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAATTCCATCAAGCTGGTGAACC
  TGCTCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGA
  CCCATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACA
  ATGTCTACAGTGGGTTACGGGGATGTTTATGCAAAAACCACGCTTGGACGCCTCTTCATGGTCTTCTTCA
  TCCTCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAA
  ATACGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGGCACATTACTCTGGAG
  AGTGTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTTTTTC
  TTCACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTA
  TCAGGGCTCTGTCCTCAATCCACACGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGTTTGATC
  CTTGCCAATAAGTACTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATAA
  AGAACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAA
  CATCCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATA
  GCCCAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAA
  AGATTGAGGAAGATACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCT
  TTCCAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTG
  ATGATAGCCATTGAGTACAAGTCTGCCAACAGGGAGAGCCGAAGCCGAAAGCGAATATTAATTAACCCTG
  GGAACCACCTTAAGACCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAG
  GGCATTTTTTTACTGCAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGCGGCTGC
  AGGCGGCTGATCTATTCCAAGATGTCCATCTACAAGAGAATGAGCCGAGCATGTTGTTTTGATTGCGGAC
  GTTCTGAGCGTGACTGCTCGTGCATGTCAGGCCGTGTGCGTGGTAACGTGGACACCCTTGAGAGAAACTT
  CCCGCTTTCTTCTGTCTCTGTTAATGATTGCTCCACCAGTTTCCGTGCCTTTGAAGATGAGCAACCGCCA
  ACACTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCACCCAACACATCCCCGAAGCTGA
  TGAGGCATGACCCCTTGTTAATTCCTGGCAACGATCAGATTGACAACATGGACTCCAATGTGAAAAAGTA
  CGACTCCACTGGAATGTTTCACTGGTGCGCACCCAAGGAGATTGAGAAAGTCATCTTGACTCGAAGTGAA
  GCTGCCATGACTGTCCTGAGTGGTCATGTTGTAGTCTGCATCTTTGGGGATGTCAGCTCAGCCCTGATTG
  GCCTCCGGAACCTGGTGATGCCGCTTCGTGCTAGCAATTTTCACTATCATGAGCTCAAACACATTGTGTT
  TGTGGGCTCCATCGAGTACCTCAAGCGGGAGTGGGAAACACTGCACAACTTCCCCAAAGTATCCATATTG
  CCTGGTACGCCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCC
  TGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTTGGCGTCACTCAA
  CATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGATTCACACCTCCT
  GGAATGGACAGATCATCACCTGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGTCCATCACAACCG
  GAGTCAACATTCCCATCATCACGGAACTCGTGAACGATACTAATGTTCAGTTTTTGGACCAAGATGATGA
  TGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTTGCCGTTAGCGTC
  CTGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGACCCTGGTGACAG
  GAGGAGCCACGCCAGAGCTCGAAGCTTTAATAGCTGAGGAGAATGCACTTCGAGGGGGTTACAGCACTCC
  GCAGACACTAGCCAACAGGGACCGCTGCCGAGTGGCCCAGTTAGCCCTGTTGGATGGGCCCTTTGCGGAC
  TTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAACATGCTTTGTTTTG
  GAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTACGTCATCACCAA
  TCCCCCCTACGAGTTTGAACTTGTACCGACAGACCTGATCTTCTGCCTGATGCAGTTTGACCACAACGCT
  GGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGAAGAGCTCCTCTG
  TCCACTCCATCCCGTCCACAGCAAATCGGCCAAACCGGCCCAAGTCCAGGGAGTCCCGCGACAAACAGAA
  AAAAGAAATGGTTTACAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202293 representing NM_031828
  Red=Cloning site Green=Tags(s)
  
  MANGGGGGGGGSSGSSGGGGGGGGGETALRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSVHEPKMDAL
  IIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQAD
  GTLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNF
  YKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRL
  IQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVT
  MSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLE
  SVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLI
  LANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFI
  AQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLL
  MIAIEYKSANRESRSRKRILINPGNHLKTQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGC
  RRLIYSKMSIYKRMSRACCFDCGRSERDCSCMSGRVRGNVDTLERNFPLSSVSVNDCSTSFRAFEDEQPP
  TLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVILTRSE
  AAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPKVSIL
  PGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPP
  GMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSV
  LDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDGPFAD
  LGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNA
  GQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKKEMVYR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031828
• ORF Size: 3729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031828.1, NP_114016.1
• RefSeq Size: 5453 bp
• RefSeq ORF: 3732 bp
• Locus ID: 83731
• UniProt ID: Q62976
• Cytogenetics: 15p16
• MW: 138.4 kDa
• Gene Summary: large-conductance Ca(2+)-activated K(+) channel; may be involved in the shortened duration of action potential in hippocampal CA1 pyramidal neurons and in channel activity in carotid body chemoreceptor cells [RGD, Feb 2006]