Wee1 (NM_001012742) Rat Tagged ORF Clone

CAT#: RR202092

Wee1 (Myc-DDK-tagged ORF) - Rat wee 1 homolog (S. pombe) (Wee1), (10 ug)

Product Images

Circular map for RR202092

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wee1
• Synonyms: MGC105683
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202092 representing NM_001012742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCCGCGCCGCAGCCGCCTGCAGTTTGCGGCAGA
  AGCTCATCTTCTCACCCGGCAGCGATTGTGAGGAGGAAGAGGAGGAGGAGGAGGAAGGCAGCGGCCACAG
  TACGGGGGAGGACTCGGCCTTCCAGGAGCCTGATTCGCCGCTGCCCTCTGCACGCAGCCCTGCCGAGGCC
  GAGGCCGAGCGCCGCCGCCGCTCGCCCGGCGCGGAGCCCAGCAGCCCGGGAGAGCTGGAGGAGGACTTGC
  TGCTGCGGGGCGGCGGCGGGGGCGCGCAAGCGGCGGGCGGAGGAGCCGAGGGCGACTCTTGGGAGGAGGA
  GGGTTTCGGCTCTTCGTCGCCCGTCAAGTCACCTACGACTGCCTACTTTCTGGGCAGCTCGTTCTCGCCG
  GTGCGCTGCGGCGGCCCTGGGGATGCCTCCCCGCGGGGCTGCGGGGTCCCGCGAGCCATGGACGACCCCT
  GTTCTCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAAGACCTTCCGCAAGTTGCGCCTGTTCGACAC
  GCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCTCGAGTTATTGATTCCAGCTCTGTTAAACTCCGGGGT
  AGTTCTCTATTCATGGACACTGAAAAATCAGGAAAAAGAGAATTCGACACACGGCAAACTCCTCAAGTGA
  ATATTAACCCCTTTACTCCGGATCCTGTAATGCTTCATTCCTCAGGACAGTGTCGTGGGAGAAAGAGAGC
  ATATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGATTATGAGTTTGAAGATGAAACAAGACCTGCC
  AAAAGAATTACAATTACTGAAAGCAATATGAAGTCACGATATACAACTGAATTTCATGAGCTGGAGAAAA
  TTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAAGAGGCTAGATGGATGCATTTATGCCATTAA
  ACGATCAAAAAAGCCATTGGCTGGCTCTGTTGATGAACAGAATGCTTTGAGAGAAGTGTATGCTCATGCT
  GTGCTTGGACAGCATCCCCATGTGGTTCGTTATTTCTCTGCCTGGGCAGAAGATGACCATATGCTTATAC
  AGAATGAATACTGTAATGGTGGGAGTTTAGCTGACGCTGTAAGTGAGAACTACAGAGTCATGAGCTACTT
  TACTGAAGCAGAGCTGAAGGATCTCCTTTTGCAAGTTGGCAGGGGCTTGAGATACATACATTCAATGTCT
  TTGGTTCACATGGATATAAAACCTAGTAATATTTTTATATCTCGAACCTCAATCCCAAATGCAGTCTCTG
  AAGAAGGAGATGAAGATGACTGGATATCCAACAAAGTTATGTTTAAAATAGGTGATCTTGGGCATGTAAC
  AAGAATCTCTAGTCCACAAGTTGAAGAAGGTGATAGTCGTTTTCTAGCAAATGAAGTTTTACAAGAGAAC
  TATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTCTCACAGTCGTATGTGCTGCTGGTGCTGAAC
  CCCTTCCCAGAAATGGAGACCAGTGGCATGAAATCCGACAGGGTCGATTACCTCGGATTCCACAAGTGCT
  TTCCCAGGAGTTGACAGAGTTGCTAAAAGTTATGATTCATCCTGATCCAGAGAGAAGACCTTCAGCAATG
  GTGCTGGTGAAGCATTCAGTGTTGCTGTCTGCCTCTAGAAAGAGCGCAGAGCAGTTACGAATAGAATTGA
  ATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACTCAAGAAAGCCCAAATGGCAGCCAAAGTTGC
  AGCTGAGGAACGGGCACTCCTCACAGATCGGATGGCCACTAGATCTACCACCCAGAGTAATAGAACTTCT
  CGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATACCGACTTATTGGAAAGAAAATGA
  ACCGCTCTGTCAGCCTTACTATATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202092 representing NM_001012742
  Red=Cloning site Green=Tags(s)
  
  MSFLSRQQPPPTRRAAAACSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEA
  EAERRRRSPGAEPSSPGELEEDLLLRGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPTTAYFLGSSFSP
  VRCGGPGDASPRGCGVPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSSSVKLRG
  SSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVMLHSSGQCRGRKRAYFNDSSEDMEASDYEFEDETRPA
  KRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHA
  VLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAVSENYRVMSYFTEAELKDLLLQVGRGLRYIHSMS
  LVHMDIKPSNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQEN
  YSHLPKADIFALALTVVCAAGAEPLPRNGDQWHEIRQGRLPRIPQVLSQELTELLKVMIHPDPERRPSAM
  VLVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALLTDRMATRSTTQSNRTS
  RLIGKKMNRSVSLTIYRLIGKKMNRSVSLTIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001012742
• ORF Size: 1938 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3305 bp
• RefSeq ORF: 1941 bp
• Locus ID: 308937
• UniProt ID: Q63802
• Cytogenetics: 1q33
• MW: 71.5 kDa
• Gene Summary: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).[UniProtKB/Swiss-Prot Function]