Ak3 (NM_013218) Rat Tagged ORF Clone

CAT#: RR202076

Ak3 (Myc-DDK-tagged ORF) - Rat adenylate kinase 3 (Ak3), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR202076

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ak3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR202076 representing NM_013218
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCATCGGGGCGGCTGCTGCGCGCCGTGATCATGGGGGCCCCGGGCTCCGGTAAGGGCACTGGGT
  CGTCACGCATCACCAAACACTTCGAGCTGAAGCACCTCTCCAGCGGGGACCTGCTCCGCCAGAACATGCT
  GCAGGGCACAGAAATCGCTGTGTTGGCCAAGAGCTTCATTGACCAAGGAAAGCTCATCCCGGATGATGAC
  ATGACTCGGCTGGCCCTCCATGAGCTGAAAAACCTTACCCAGTGTAGCTGGCTGTTGGACGGATTTCCAA
  GGACACTTCCACAGGCAGAAGCCCTGGATAGAGTTTATCAGATAGACACAGTGATAAATCTCAACGTGCC
  CTTTGAGGTCATTAAACTACGCCTTACTGCTCGCTGGATTCATCCTGCCAGTGGCCGAGTTTACAACATT
  GAATTCAACCCTCCCAAGACTGTGGGCATCGATGACCTAACGGGAGAACCTCTGATTCAGCGTGAGGACG
  ACAAACCAGAGACGGTGATCAAGAGATTGAAAGCGTATGAAGCCCAGACAGAGCCGGTCCTGCAGTATTA
  CCAGAAAAAAGGGGTGTTGGAAACATTCTCCGGAACAGAAACCAACAAGATCAGGCCCCACGTATACTCC
  TTCCTGCAAATGAAAGTTCCAGAAACCATCCAAAAAGCCTCTGTTACTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR202076 representing NM_013218
  Red=Cloning site Green=Tags(s)
  
  MGASGRLLRAVIMGAPGSGKGTGSSRITKHFELKHLSSGDLLRQNMLQGTEIAVLAKSFIDQGKLIPDDD
  MTRLALHELKNLTQCSWLLDGFPRTLPQAEALDRVYQIDTVINLNVPFEVIKLRLTARWIHPASGRVYNI
  EFNPPKTVGIDDLTGEPLIQREDDKPETVIKRLKAYEAQTEPVLQYYQKKGVLETFSGTETNKIRPHVYS
  FLQMKVPETIQKASVTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013218
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013218.1, NP_037350.1
• RefSeq Size: 1061 bp
• RefSeq ORF: 684 bp
• Locus ID: 26956
• UniProt ID: P29411
• Cytogenetics: 1q52
• MW: 25.4 kDa
• Gene Summary: Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.[UniProtKB/Swiss-Prot Function]