Thbs1 (NM_001013062) Rat Tagged ORF Clone

CAT#: RR201766

Thbs1 (Myc-DDK-tagged ORF) - Rat thrombospondin 1 (Thbs1), (10 ug)

Product Images

Circular map for RR201766

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Thbs1
• Synonyms: TSP-1; Tsp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201766 representing NM_001013062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTCAGGGGACTAGGCGTCCTGTTCCTGTTGCATGTGTGTGGAAGCAACCGCATTCCAGAGT
  CTGGGGGAGACAATGGTGTGTTCGACATTTTTGAACTCATTGGAGGTGCCCGCAAGGTTCCGGGTCGCCG
  ACTGGTGAAGGGCCAAGATCTATCCAGCCCCGCCTTCCGGATCGAGAATGCCAACCTGATCCCCCCTGTG
  CCAGATGACAAGTTCCAAGACCTACTGGACGCTGTGTGGGCCGACAAAGGCTTCATCTTCCTGGCTTCCT
  TGAGGCAGATGAAGAAGACTCGGGGCACACTCCTGGCCGTGGAACGGAAAGACAATTCTGGCCAGATCTT
  CAGTGTGGTCTCCAATGGCAAAGCCGGCACCCTCGATCTGAGTCTGAGCCTGCCCGGGAAGCAGCAAGTG
  GTGTCAGTGGAGGAAGCTCTCCTGGCCACTGGCCAGTGGAAGAGCATCACGCTGTTTGTCCAAGAAGACA
  GGGCCCAGCTCTACATTGACTGTGACAAGATGGAGAGCGCAGAGCTGGATGTTCCCATCCAGAGCATCTT
  CACAAGGGATTTGGCCAATGTCGCCAGGCTCCGAGTCGCAAAGGGAGATGTCAATGACAATTTTCAGGGG
  GTGCTGCAGAATGTGAGGTTTGTCTTTGGAACCACCCCAGAAGACATTCTCAGGAACAAAGGCTGCTCCA
  GTTCAACCAACGTCCTTCTCACCCTTGACAACAACGTGGTGAACGGTTCCAGCCCTGCCATCCGCACCAA
  CTACATCGGCCACAAAACAAAGGACCTCCAAGCCATCTGTGGCCTCTCCTGTGACGAACTATCCAGCATG
  GTCCTGGAACTGAGGGGCCTGCGTACCATCGTGACCACGCTACAGGACAGCATTCGCAAAGTGACGGAAG
  AGAACAGAGAGCTGGCTAGCGAGCTGAGGCGGCCTCCCCTCTGCTTCCACAATGGAGTCCAATACAGGAA
  CAACGAGGAGTGGACTGTAGATAGTTGCACAGAGTGTCACTGCCAGAACTCAGTTACCATCTGCAAAAAG
  GTGTCCTGTCCCATCATGCCCTGCTCCAATGCCACAGTTCCTGATGGTGAATGCTGCCCACGGTGCTGGC
  CCAGTGACTCTGCTGACGATGGCTGGTCTCCCTGGTCTGAGTGGACCTCTTGCTCTGCCACCTGTGGCAA
  TGGGATTCAGCAACGTGGTCGCTCCTGTGACAGCCTCAACAACAGATGTGAGGGCTCTTCAGTACAGACG
  AGGACCTGCCACATTCAGGAGTGCGACAAAAGATTTAAACAGGATGGTGGTTGGAGTCACTGGTCCCCGT
  GGTCGTCCTGTTCTGTGACCTGTGGTGACGGTGTGATCACGAGGATCCGACTCTGCAACTCCCCCAGCCC
  CCAGATGAACGGGAAGCCCTGTGAAGGTGAAGCTCGGGAGACCAAAGCCTGCAAGAAGGACGCCTGCCCA
  ATCAATGGAGGCTGGAGTCCCTGGTCACTATGGGACATCTGCTCTGTCACCTGTGGAGGAGGAGTACAGA
  GACGTAGCCGACTCTGCAACAACCCCACACCCCAGTTTGGAGGCAAAGACTGTGTTGGTGATGTGACGGA
  AAATCAAGTTTGCAACAAGCAGGACTGTCCGATTGATGGATGCCTGTCCAATCCCTGCTTTGCTGGTGCC
  AAGTGTACGAGCTATCCTGATGGTAGCTGGAAATGTGGTGCGTGTCCTCCTGGCTACAGTGGAAATGGTA
  TCCAGTGCAAAGACGTCGACGAGTGCAAAGAAGTGCCTGATGCTTGCTTCAATCACAACGGGGAACATCG
  GTGCAAGAACACAGATCCTGGCTACAACTGCCTGCCCTGCCCACCACGATTCACCGGCTCACAGCCCTTC
  GGCAGAGGTGTCGAACACGCTATGGCCAACAAACAGGTGTGCAAACCCCGAAACCCCTGCACCGACGGGA
  CACACGACTGCAACAAGAACGCCAAGTGCAACTACCTGGGTCACTACAGCGACCCCATGTACCGCTGCGA
  GTGCAAGCCTGGCTATGCAGGCAATGGCATCATTTGCGGAGAGGACACAGACCTTGACGGCTGGCCTAAT
  GAAAACCTGGTGTGTGTGGCCAACGCAACCTACCACTGCAAAAAGGACAACTGCCCCAATCTTCCCAATT
  CGGGGCAGGAAGACTATGACAAGGATGGGATTGGCGATGCCTGTGATGACGATGATGACAACGACAAGAT
  TCCTGATGACAGGGACAACTGTCCATTCCATTACAACCCTGCCCAGTATGACTATGACAGAGATGATGTG
  GGAGACCGCTGTGACAATTGCCCCTACAACCACAACCCTGACCAGGCAGACACAGACAACAATGGGGAGG
  GAGATGCCTGCGCTGTGGACATCGATGGGGATGGAATCCTCAATGAACGAGACAACTGCCAGTATGTTTA
  CAACGTGGATCAGAGGGACACGGACATGGATGGGGTTGGAGACCAGTGTGACAACTGCCCCCTGGAACAC
  AATCCAGACCAGCTGGACTCTGACTCGGACCGCATAGGGGACACCTGTGACAACAATCAGGCCATCGATG
  AGGATGGCCATCAGAACAACCTTGAAAACTGTCCCTATGTGCCCAATGCCAACCAGGCCGACCACGATAA
  AGATGGTAAAGGAGACGCCTGTGACCATGACGATGACAACGACGGCATCCCTGATGACAGAGACAACTGC
  AGGCTGGTGCCCAATCCTGACCAGAAGGACTCTGATGGTGATGGCCGAGGCGATGCCTGCAAAGACGACT
  TTGACCATGACAATGTGCCAGACATTGATGACATCTGTCCTGAGAATGTTGACATCAGTGAAACCGATTT
  CCGCCGATTCCAGATGATTCCTCTAGATCCCAAAGGAACCTCCCAAAATGACCCTAACTGGGTTGTCCGC
  CATCAGGGCAAAGAACTTGTCCAGACTGTAAACTGTGACCCTGGACTTGCTGTAGGTTATGATGAGTTTA
  ATGCCGTGGACTTCAGTGGTACCTTCTTCATCAACACTGAGAGGGATGACGACTATGCTGGCTTTGTTTT
  CGGGTACCAGTCCAGCAGCCGCTTCTACGTTGTGATGTGGAAACAAGTCACCCAGTCCTACTGGGACACC
  AACCCCACAAGGGCTCAGGGATACTCAGGCCTGTCTGTAAAGGTTGTAAACTCCACCACTGGCCCCGGCG
  AGCACCTGCGGAATGCACTGTGGCACACAGGAAACACCCCTGGCCAGGTGCGCACCCTGTGGCATGACCC
  TCGTCACATTGGCTGGAAAGATTTCACTGCATACAGATGGCGTCTCAGCCACAGGCCAAAGACCGGTTTG
  ATCAGAGTGGTGATGTATGAAGGAAAGAAAATCATGGCTGACTCAGGACCCATCTATGACAAAACCTACG
  CTGGCGGTAGACTAGGCCTGTTCGTCTTCTCTCAAGAAATGGTGTTCTTCTCGGACATGAAATACGAGTG
  CCGAGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201766 representing NM_001013062
  Red=Cloning site Green=Tags(s)
  
  MELLRGLGVLFLLHVCGSNRIPESGGDNGVFDIFELIGGARKVPGRRLVKGQDLSSPAFRIENANLIPPV
  PDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNSGQIFSVVSNGKAGTLDLSLSLPGKQQV
  VSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLANVARLRVAKGDVNDNFQG
  VLQNVRFVFGTTPEDILRNKGCSSSTNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSM
  VLELRGLRTIVTTLQDSIRKVTEENRELASELRRPPLCFHNGVQYRNNEEWTVDSCTECHCQNSVTICKK
  VSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSATCGNGIQQRGRSCDSLNNRCEGSSVQT
  RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACP
  INGGWSPWSLWDICSVTCGGGVQRRSRLCNNPTPQFGGKDCVGDVTENQVCNKQDCPIDGCLSNPCFAGA
  KCTSYPDGSWKCGACPPGYSGNGIQCKDVDECKEVPDACFNHNGEHRCKNTDPGYNCLPCPPRFTGSQPF
  GRGVEHAMANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPN
  ENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDV
  GDRCDNCPYNHNPDQADTDNNGEGDACAVDIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH
  NPDQLDSDSDRIGDTCDNNQAIDEDGHQNNLENCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDRDNC
  RLVPNPDQKDSDGDGRGDACKDDFDHDNVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVR
  HQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDT
  NPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGL
  IRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDMKYECRDS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013062
• ORF Size: 3510 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013062.1, NP_001013080.1
• RefSeq Size: 3734 bp
• RefSeq ORF: 3513 bp
• Locus ID: 445442
• Cytogenetics: 3q35
• MW: 129.7 kDa