Thbs1 (NM_001013062) Rat Untagged Clone

CAT#: RN201766

Thbs1 (untagged ORF) - Rat thrombospondin 1 (Thbs1), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Thbs1
• Synonyms: TSP-1; Tsp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN201766 representing NM_001013062
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCCTCAGGGGACTAGGCGTCCTGTTCCTGTTGCATGTGTGTGGAAGCAACCGCATTCCAGAGT
  CTGGGGGAGACAATGGTGTGTTCGACATTTTTGAACTCATTGGAGGTGCCCGCAAGGTTCCGGGTCGCCG
  ACTGGTGAAGGGCCAAGATCTATCCAGCCCCGCCTTCCGGATCGAGAATGCCAACCTGATCCCCCCTGTG
  CCAGATGACAAGTTCCAAGACCTACTGGACGCTGTGTGGGCCGACAAAGGCTTCATCTTCCTGGCTTCCT
  TGAGGCAGATGAAGAAGACTCGGGGCACACTCCTGGCCGTGGAACGGAAAGACAATTCTGGCCAGATCTT
  CAGTGTGGTCTCCAATGGCAAAGCCGGCACCCTCGATCTGAGTCTGAGCCTGCCCGGGAAGCAGCAAGTG
  GTGTCAGTGGAGGAAGCTCTCCTGGCCACTGGCCAGTGGAAGAGCATCACGCTGTTTGTCCAAGAAGACA
  GGGCCCAGCTCTACATTGACTGTGACAAGATGGAGAGCGCAGAGCTGGATGTTCCCATCCAGAGCATCTT
  CACAAGGGATTTGGCCAATGTCGCCAGGCTCCGAGTCGCAAAGGGAGATGTCAATGACAATTTTCAGGGG
  GTGCTGCAGAATGTGAGGTTTGTCTTTGGAACCACCCCAGAAGACATTCTCAGGAACAAAGGCTGCTCCA
  GTTCAACCAACGTCCTTCTCACCCTTGACAACAACGTGGTGAACGGTTCCAGCCCTGCCATCCGCACCAA
  CTACATCGGCCACAAAACAAAGGACCTCCAAGCCATCTGTGGCCTCTCCTGTGACGAACTATCCAGCATG
  GTCCTGGAACTGAGGGGCCTGCGTACCATCGTGACCACGCTACAGGACAGCATTCGCAAAGTGACGGAAG
  AGAACAGAGAGCTGGCTAGCGAGCTGAGGCGGCCTCCCCTCTGCTTCCACAATGGAGTCCAATACAGGAA
  CAACGAGGAGTGGACTGTAGATAGTTGCACAGAGTGTCACTGCCAGAACTCAGTTACCATCTGCAAAAAG
  GTGTCCTGTCCCATCATGCCCTGCTCCAATGCCACAGTTCCTGATGGTGAATGCTGCCCACGGTGCTGGC
  CCAGTGACTCTGCTGACGATGGCTGGTCTCCCTGGTCTGAGTGGACCTCTTGCTCTGCCACCTGTGGCAA
  TGGGATTCAGCAACGTGGTCGCTCCTGTGACAGCCTCAACAACAGATGTGAGGGCTCTTCAGTACAGACG
  AGGACCTGCCACATTCAGGAGTGCGACAAAAGATTTAAACAGGATGGTGGTTGGAGTCACTGGTCCCCGT
  GGTCGTCCTGTTCTGTGACCTGTGGTGACGGTGTGATCACGAGGATCCGACTCTGCAACTCCCCCAGCCC
  CCAGATGAACGGGAAGCCCTGTGAAGGTGAAGCTCGGGAGACCAAAGCCTGCAAGAAGGACGCCTGCCCA
  ATCAATGGAGGCTGGAGTCCCTGGTCACTATGGGACATCTGCTCTGTCACCTGTGGAGGAGGAGTACAGA
  GACGTAGCCGACTCTGCAACAACCCCACACCCCAGTTTGGAGGCAAAGACTGTGTTGGTGATGTGACGGA
  AAATCAAGTTTGCAACAAGCAGGACTGTCCGATTGATGGATGCCTGTCCAATCCCTGCTTTGCTGGTGCC
  AAGTGTACGAGCTATCCTGATGGTAGCTGGAAATGTGGTGCGTGTCCTCCTGGCTACAGTGGAAATGGTA
  TCCAGTGCAAAGACGTCGACGAGTGCAAAGAAGTGCCTGATGCTTGCTTCAATCACAACGGGGAACATCG
  GTGCAAGAACACAGATCCTGGCTACAACTGCCTGCCCTGCCCACCACGATTCACCGGCTCACAGCCCTTC
  GGCAGAGGTGTCGAACACGCTATGGCCAACAAACAGGTGTGCAAACCCCGAAACCCCTGCACCGACGGGA
  CACACGACTGCAACAAGAACGCCAAGTGCAACTACCTGGGTCACTACAGCGACCCCATGTACCGCTGCGA
  GTGCAAGCCTGGCTATGCAGGCAATGGCATCATTTGCGGAGAGGACACAGACCTTGACGGCTGGCCTAAT
  GAAAACCTGGTGTGTGTGGCCAACGCAACCTACCACTGCAAAAAGGACAACTGCCCCAATCTTCCCAATT
  CGGGGCAGGAAGACTATGACAAGGATGGGATTGGCGATGCCTGTGATGACGATGATGACAACGACAAGAT
  TCCTGATGACAGGGACAACTGTCCATTCCATTACAACCCTGCCCAGTATGACTATGACAGAGATGATGTG
  GGAGACCGCTGTGACAATTGCCCCTACAACCACAACCCTGACCAGGCAGACACAGACAACAATGGGGAGG
  GAGATGCCTGCGCTGTGGACATCGATGGGGATGGAATCCTCAATGAACGAGACAACTGCCAGTATGTTTA
  CAACGTGGATCAGAGGGACACGGACATGGATGGGGTTGGAGACCAGTGTGACAACTGCCCCCTGGAACAC
  AATCCAGACCAGCTGGACTCTGACTCGGACCGCATAGGGGACACCTGTGACAACAATCAGGCCATCGATG
  AGGATGGCCATCAGAACAACCTTGAAAACTGTCCCTATGTGCCCAATGCCAACCAGGCCGACCACGATAA
  AGATGGTAAAGGAGACGCCTGTGACCATGACGATGACAACGACGGCATCCCTGATGACAGAGACAACTGC
  AGGCTGGTGCCCAATCCTGACCAGAAGGACTCTGATGGTGATGGCCGAGGCGATGCCTGCAAAGACGACT
  TTGACCATGACAATGTGCCAGACATTGATGACATCTGTCCTGAGAATGTTGACATCAGTGAAACCGATTT
  CCGCCGATTCCAGATGATTCCTCTAGATCCCAAAGGAACCTCCCAAAATGACCCTAACTGGGTTGTCCGC
  CATCAGGGCAAAGAACTTGTCCAGACTGTAAACTGTGACCCTGGACTTGCTGTAGGTTATGATGAGTTTA
  ATGCCGTGGACTTCAGTGGTACCTTCTTCATCAACACTGAGAGGGATGACGACTATGCTGGCTTTGTTTT
  CGGGTACCAGTCCAGCAGCCGCTTCTACGTTGTGATGTGGAAACAAGTCACCCAGTCCTACTGGGACACC
  AACCCCACAAGGGCTCAGGGATACTCAGGCCTGTCTGTAAAGGTTGTAAACTCCACCACTGGCCCCGGCG
  AGCACCTGCGGAATGCACTGTGGCACACAGGAAACACCCCTGGCCAGGTGCGCACCCTGTGGCATGACCC
  TCGTCACATTGGCTGGAAAGATTTCACTGCATACAGATGGCGTCTCAGCCACAGGCCAAAGACCGGTTTG
  ATCAGAGTGGTGATGTATGAAGGAAAGAAAATCATGGCTGACTCAGGACCCATCTATGACAAAACCTACG
  CTGGCGGTAGACTAGGCCTGTTCGTCTTCTCTCAAGAAATGGTGTTCTTCTCGGACATGAAATACGAGTG
  CCGAGACTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013062
• Insert Size: 3513 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013062.1, NP_001013080.1
• RefSeq Size: 3734 bp
• RefSeq ORF: 3513 bp
• Locus ID: 445442
• Cytogenetics: 3q35