Tmprss9 (NM_001047100) Rat Tagged ORF Clone

CAT#: RR201644

Tmprss9 (Myc-DDK-tagged ORF) - Rat transmembrane protease, serine 9 (Tmprss9), (10 ug)

Product Images

Circular map for RR201644

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmprss9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201644 representing NM_001047100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAGCGGCACCAGACTTACAACCTGTGCCAGAGGTGACGAAGGGTGTCCCCGTCCCCACTCCTG
  ACTCTGGCTGTTGTCGAGCCGCTGTCACCACAGTGGTGGCCATCAGCGTGGCCAGCCTCACCCTAGGAGT
  CCTTTCAGCCTTCCTCTCTGCACAGGGTGTCCAGGTGGAGCACACGGCGCAGCTACATGGGGTCCGTTTC
  ACCAGCCTTTTGCAGCAGGAGAACTCCGACTTCTACCGCCTGCTTACGCCTGCTCTGCAGACGCTGCTAC
  ACTTTCTGCTCCGAGCCCTCCAGCCACTGAGCCTGGACCAGGAAGCAGACATCTTGCAGAAAGGAATCCA
  GGCAAGGTTACAAGGACAAGGTCTTTCCCTGGCTGCCTACGGCACCATCACATCCGTGGAGCTGACAGGA
  AGGTGTGAAGGTCCGGTGACCGAGAGGGACTTAAAGTCAGGCCACTGCCCCGGGAATGCCTTCTCCTGTC
  AGAACAGTCAATGCGTGAGCAAAGAAAACCCGGAGTGTGACGACAGAGTGGACTGCTCTGATGGGTCTGA
  CGAGGCTCAGTGTGACTGCGGCTGGCAGCCTGCCTGGAGATCAGCTGGAAGGATCGTGGGCGGAGCGGAG
  GCGGCTCCGGGAGAGTTCCCCTGGCAGGTCAGCCTCCGAGAGAATCACGAACACTTCTGCGGCGCCACCA
  TCATCGGTGCCCGGTGGCTGGTGTCTGCTGCCCACTGCTTCAACGAATTCCAGGACCCTGCGCAATGGGC
  CGCCCAGGCAGGCAGCGTGCACCTGAGCGGTTCAGAGGCCAGCGCTGTGCGTGCGCGCGTGCTCCGTATC
  GCCAAGCATCCTGCCTACAACGCGGACACGGCGGACTTTGACGTGGCGGTGCTGGAACTGGCTCGCCCGC
  TGCCCTTCGGCCGCTATGTGCAGCCTGCCTGCCTTCCCGCGGCCACTCACGTCTTCCCACCGCGCAAGAA
  GTGCCTGATCTCGGGCTGGGGTTACCTGAAGGAGGACTTCCTGGTGAAGCCAGAGGTCCTTCAGAAAGCC
  ACAGTGGAATTGCTGGACCAGAACCTGTGTTCCAGCCTGTATGGCCACTCGCTCACGGACAGGATGGTGT
  GTGCCGGCTACCTGGACGGGAAGGTGGACTCCTGCCAGGGTGACTCCGGGGGACCCCTGGTCTGCGAGGA
  GCCTTCCGGAAGGTTCTTCCTGGCTGGTGTCGTGAGCTGGGGAATCGGCTGTGCAGAAGCACGGCGCCCA
  GGGGTCTACACCCGAGTCACCAGGCTGCGGGATTGGATCTTGGAGGTGACCAGCTCGGCTGACACGCCCG
  TGGTCCCCACCGAGGCTCCAGCACCCATCACCCCCAGCACTCCCTGGCCTACCAGCCCCGAGAGCCGGGT
  GCCCAACACCACTGCTAAACCCACCGTTGCCCCCACCCCTGCGCCACTGCACCCCAGCACTGCGGCCAAG
  CCACAAGAGTGTGGGGCCAGGCCCGCGATGGACAAGCCCACTCGCATTGTGGGCGGGATCAGCGCCGTGT
  CCGGGGAGGTGCCCTGGCAGGCCAGCCTGAAGGAAGGATCCAGACATTTCTGCGGAGCCACCGTGGTGGG
  TGACCGCTGGCTGCTGTCCGCTGCCCACTGTTTCAACCACACCAAACTGGAACAGGTGCAGGCGCACTTG
  GGCACCGTGTCCCTGCTGGGTGTCGGCGGGAGCCCCGTGAAGCTGGGGCTGCGAAGCGTGGCCCTGCACC
  CCCGCTACAACCCAGGAATCCTGGACTTTGACGTGGCCTTGCTGGAGTTGGCCCAGCCATTGGTCTTCAA
  CAAGTACATCCAGCCAGTCTGTCTCCCGCTGGCCATCCACAAGTTCCCCGTGGGCCGGAAGTGTATGATC
  TCGGGATGGGGCAACATGCAGGAGGGAAACGCCACCAAGCCCGACATCTTGCAGAAGGCGTCTGTGGGCA
  TCATAGAGCAGAAGATGTGTGGTGCCCTCTACAACTTCTCCCTCACCGACCGCATGCTCTGCGCCGGCTT
  CCTGGAGGGTAGAGTCGACTCCTGCCAGGGTGATTCTGGTGGCCCCCTGGCATGTGAGGAGACACCTGGT
  GTCTTTTACCTGGCTGGCATCGTGAGCTGGGGCATTGGCTGTGCCCAAGCTAAGAAGCCTGGTGTATATG
  CACGAATCACGCGCCTCAAGGACTGGATCCTGAAAGCCATGTCCTCAGACCCCAGCTCTACGGCTCACCC
  CCACACCTCCAGCACACGGCTCATCCCTAGCCAGCCCCCAACAACCACAGCTGCTGGCCTCATCCCAGAG
  GCCAGCACAGGCAGACCTGCTACCCTCAGAGCCACCATCAGAGTGACTACTAGACCACTCAACACAACGT
  TGTCTGCCAGGAGTACCACCACCAGGAGACAGACACCAGCACCGGGCACCACTGTGTTCTCTCATCTGCC
  AGACTGCGGCCTGGCACCCCCAGGGGCCCTGACCCGGATAGTGGGCGGCAGCGCAGCCTCGCTTGGAGAA
  TGGCCCTGGCAAGTGAGCCTGTGGCTGCGGCGCAGGGAGCACCGCTGTGGGGCGGTACTGGTGGCCGAGA
  GGTGGCTTCTGTCTGCAGCACACTGTTTCGACGTCTATGGGGACCCTATGCAGTGGGCAGCCTTCCTGGG
  GACCCCGTTCCTGAGCAGCACGGAGGGCCAGTTGGAGCGTGTGGCACGCATCTATCGCCACCCGTTCTAC
  AACATCTACACGCTGGACTATGACGTGGCGCTGCTGGAGCTGGCGGGGCCTGTGCGCCGTAGCCGCTTGG
  TGCGACCCATCTGCCTACCAGGTCCCACTCGTCCTCCTGAAGGCGCGCGCTGTGTCATCACTGGCTGGGG
  TTCGCTGCGAGAAGGAGGCTCCATGGCGAGGCAGCTGCAGAAGGCTGCGGTGAGGGTCCTCAGTGAACAG
  ACATGTCGCCGCTTCTACCCGGTACAGATCAGCAGTCGTATGTTGTGCGCAGGCTTCCCGCAGGGTGGCG
  TGGACAGCTGCTCGGGTGATGCAGGGGGACCCCTGGCCTGCAGGGAGCCATCTGGCCAATGGGTGCTAAC
  TGGGGTCACCAGTTGGGGCTACGGCTGTGGCAGGCCGCACTTCCCAGGTGTCTACACTCGAGTGGCTGCT
  GTACTAGGATGGATAGGCCAGAATATCCGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201644 representing NM_001047100
  Red=Cloning site Green=Tags(s)
  
  MEPAAPDLQPVPEVTKGVPVPTPDSGCCRAAVTTVVAISVASLTLGVLSAFLSAQGVQVEHTAQLHGVRF
  TSLLQQENSDFYRLLTPALQTLLHFLLRALQPLSLDQEADILQKGIQARLQGQGLSLAAYGTITSVELTG
  RCEGPVTERDLKSGHCPGNAFSCQNSQCVSKENPECDDRVDCSDGSDEAQCDCGWQPAWRSAGRIVGGAE
  AAPGEFPWQVSLRENHEHFCGATIIGARWLVSAAHCFNEFQDPAQWAAQAGSVHLSGSEASAVRARVLRI
  AKHPAYNADTADFDVAVLELARPLPFGRYVQPACLPAATHVFPPRKKCLISGWGYLKEDFLVKPEVLQKA
  TVELLDQNLCSSLYGHSLTDRMVCAGYLDGKVDSCQGDSGGPLVCEEPSGRFFLAGVVSWGIGCAEARRP
  GVYTRVTRLRDWILEVTSSADTPVVPTEAPAPITPSTPWPTSPESRVPNTTAKPTVAPTPAPLHPSTAAK
  PQECGARPAMDKPTRIVGGISAVSGEVPWQASLKEGSRHFCGATVVGDRWLLSAAHCFNHTKLEQVQAHL
  GTVSLLGVGGSPVKLGLRSVALHPRYNPGILDFDVALLELAQPLVFNKYIQPVCLPLAIHKFPVGRKCMI
  SGWGNMQEGNATKPDILQKASVGIIEQKMCGALYNFSLTDRMLCAGFLEGRVDSCQGDSGGPLACEETPG
  VFYLAGIVSWGIGCAQAKKPGVYARITRLKDWILKAMSSDPSSTAHPHTSSTRLIPSQPPTTTAAGLIPE
  ASTGRPATLRATIRVTTRPLNTTLSARSTTTRRQTPAPGTTVFSHLPDCGLAPPGALTRIVGGSAASLGE
  WPWQVSLWLRRREHRCGAVLVAERWLLSAAHCFDVYGDPMQWAAFLGTPFLSSTEGQLERVARIYRHPFY
  NIYTLDYDVALLELAGPVRRSRLVRPICLPGPTRPPEGARCVITGWGSLREGGSMARQLQKAAVRVLSEQ
  TCRRFYPVQISSRMLCAGFPQGGVDSCSGDAGGPLACREPSGQWVLTGVTSWGYGCGRPHFPGVYTRVAA
  VLGWIGQNIRE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001047100
• ORF Size: 3183 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001047100.2, NP_001040565.2
• RefSeq Size: 3341 bp
• RefSeq ORF: 3186 bp
• Locus ID: 314636
• UniProt ID: P69526
• Cytogenetics: 7q11
• MW: 113.9 kDa
• Gene Summary: Serase-1 and serase-2 are serine proteases that hydrolyze the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC. In contrast, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Ala-Pro-Ala-AMC are not significantly hydrolyzed (By similarity).[UniProtKB/Swiss-Prot Function]