Grm5 (NM_017012) Rat Tagged ORF Clone

CAT#: RR201568

Grm5 (Myc-DDK-tagged ORF) - Rat glutamate receptor, metabotropic 5 (Grm5), (10 ug)

Product Images

Circular map for RR201568

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grm5
• Synonyms: mGluR5; mGlur5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201568 representing NM_017012
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTTCTGTTGATCCTGTCAGTCCTACTTCTGAAAGAAGATGTACGAGGGAGTGCACAGTCCAGTG
  AGAGGAGGGTGGTGGCTCACATGCCAGGTGACATCATTATTGGAGCTCTCTTCTCTGTCCACCACCAACC
  AACTGTGGACAAAGTTCATGAGAGGAAGTGTGGTGCAGTTCGTGAGCAATATGGGATTCAGAGAGTGGAG
  GCCATGCTGCATACCTTGGAAAGGATCAACTCAGATCCCACACTCTTGCCCAACATCACACTTGGCTGTG
  AGATCAGAGATTCCTGCTGGCATTCCGCTGTGGCCCTAGAACAAAGCATTGAGTTTATAAGGGATTCCCT
  CATCTCTTCAGAAGAGGAGGAAGGTTTGGTACGCTGTGTAGATGGATCTTCTTCCTTCCGCTCCAAGAAA
  CCCATAGTGGGAGTCATTGGGCCTGGCTCGAGTTCTGTGGCCATTCAAGTCCAGAACTTACTCCAGCTTT
  TCAACATACCACAGATTGCTTACTCTGCAACCAGCATGGATTTGAGTGACAAGACTCTATTCAAGTACTT
  CATGAGGGTTGTACCTTCCGATGCCCAGCAAGCTCGAGCCATGGTAGACATAGTGAAGAGATACAACTGG
  ACTTATGTCTCAGCTGTGCACACAGAAGGCAACTATGGAGAAAGTGGGATGGAGGCTTTCAAAGATATGT
  CAGCGAAGGAAGGGATTTGCATCGCCCACTCTTACAAAATTTACAGCAATGCTGGGGAACAGAGCTTTGA
  CAAGCTGTTGAAAAAACTCAGAAGTCATTTACCTAAAGCCCGGGTGGTAGCTTGCTTCTGTGAAGGCATG
  ACAGTTCGAGGTCTGCTCATGGCCATGAGACGCTTAGGTCTAGCAGGGGAGTTTCTACTTCTGGGCAGTG
  ATGGCTGGGCCGACAGGTATGACGTGACAGATGGATATCAGCGAGAAGCTGTCGGTGGAATTACAATCAA
  GCTTCAGTCTCCTGATGTCAAGTGGTTTGATGATTATTATCTGAAACTCCGGCCAGAAACAAACCTCAGA
  AACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGGCTGGAAGGGTTTGCACAGGAGAACA
  GCAAGTACAACAAGACTTGCAACAGTTCTCTGACTCTGAGAACACATCATGTTCAAGATTCCAAAATGGG
  ATTTGTGATAAATGCAATCTATTCTATGGCTTATGGACTCCACAATATGCAGATGTCCCTGTGTCCAGGC
  TATGCAGGCCTCTGTGATGCGATGAAGCCAATTGATGGACGGAAACTTTTGGATTCCCTGATGAAAACCA
  ATTTTACTGGAGTTTCTGGAGATATGATCCTGTTTGATGAAAATGGAGACTCTCCAGGAAGGTATGAAAT
  AATGAATTTCAAGGAAATGGGAAAAGATTATTTTGATTACATCAATGTTGGAAGTTGGGACAATGGGGAA
  TTAAAAATGGATGATGACGAAGTGTGGTCCAAGAAAAATAACATCATCAGATCTGTGTGCAGTGAACCAT
  GTGAGAAAGGCCAAATAAAGGTGATCCGGAAGGGAGAAGTCAGCTGTTGTTGGACCTGCACACCTTGTAA
  AGAGAATGAGTATGTGTTTGATGAGTACACCTGCAAGGCATGCCAACTGGGCTCCTGGCCCACTGACGAC
  CTGACAGGCTGTGACTTGATCCCAGTCCAGTATCTTCGCTGGGGTGACCCTGAGCCGATTGCAGCTGTGG
  TGTTTGCCTGCCTCGGTCTGCTAGCCACCTTATTCGTTACTGTAATCTTCATCATTTATCGGGACACTCC
  GGTGGTCAAGTCCTCCAGCAGGGAGCTCTGCTACATTATCCTTGCTGGCATCTGCCTGGGATACTTATGT
  ACCTTTTGCCTCATTGCAAAGCCCAAGCAGATTTACTGCTATCTTCAGAGAATTGGCATTGGACTCTCTC
  CAGCCATGAGCTACTCAGCCCTTGTAACCAAGACCAACCGTATTGCAAGGATTCTAGCTGGAAGCAAGAA
  GAAAATCTGTACCAAGAAGCCCAGATTCATGAGCGCCTGTGCTCAGTTAGTGATCGCTTTCATTCTCATC
  TGTATTCAGCTGGGCATTATCGTGGCCCTCTTTATCATGGAGCCTCCGGATATAATGCATGACTATCCAA
  GCATCCGAGAAGTCTACTTGATTTGTAACACCACCAACCTAGGGGTTGTTACTCCTCTTGGATACAATGG
  ATTATTGATTTTGAGTTGCACGTTCTATGCGTTTAAGACCAGAAATGTTCCAGCCAACTTTAATGAGGCC
  AAATATATTGCTTTCACCATGTACACAACCTGCATCATATGGCTGGCCTTCGTGCCTATCTACTTTGGCA
  GCAACTACAAAATCATCACCATGTGTTTTTCAGTCAGCCTCAGTGCCACAGTGGCCCTGGGTTGCATGTT
  TGTCCCGAAGGTGTACATCATCCTAGCCAAACCGGAGAGAAATGTGCGCAGCGCCTTCACAACCTCTACA
  GTGGTACGCATGCATGTAGGAGACGGCAAATCATCGTCCGCTGCCAGCAGATCCAGCAGCCTAGTCAACC
  TGTGGAAGAGGAGGGGCTCCTCTGGGGAAACCCTAAGCTCCAACGGAAAATCTGTGACTTGGGCCCAGAA
  TGAGAAGAGTACCCGGGGGCAACATTTGTGGCAGCGACTGTCTGTCCACATCAACAAGAAGGAGAACCCC
  AACCAAACGGCGGTCATCAAACCCTTTCCCAAGAGCACAGAGAACCGGGGGCCTGGTGCAGCGGCAGGTG
  GTGGCTCGGGTCCCGGTGTAGCTGGTGCTGGTAACGCAGGATGCACAGCAACAGGTGGCCCGGAGCCACC
  GGACGCCGGTCCCAAAGCGCTTTATGATGTGGCGGAGGCAGAGGAGAGCTTCCCCGCGGCTGCCAGGCCG
  CGCTCGCCATCGCCCATCAGCACGTTGAGCCACCTCGCAGGCTCGGCGGGCCGCACAGATGATGATGCGC
  CGTCGCTGCACTCTGAGACAGCTGCTCGCAGCAGCTCGTCCCAGGGCTCGCTCATGGAGCAGATCAGCAG
  CGTAGTGACGCGCTTCACAGCCAACATCTCCGAGCTCAACTCCATGATGTTGTCCACCGCGGCCACGCCG
  GGGCCCCCTGGTACTCCAATCTGCTCCTCCTACCTGATCCCCAAAGAGATTCAGCTGCCCACGACCATGA
  CGACCTTCGCAGAGATCCAGCCGCTGCCGGCCATCGAGGTGACCGGAGGAGCTCAGGGGGCGACAGGCGT
  ATCACCTGCCCAGGAGACGCCCACAGGAGCTGAATCCGCCCCGGGCAAACCAGATCTGGAGGAGCTTGTG
  GCCCTCACTCCACCATCGCCCTTCAGGGACTCGGTGGACTCGGGGAGCACCACCCCAAACTCTCCAGTCT
  CAGAATCGGCCCTCTGCATCCCATCCTCTCCCAAATATGACACTCTCATCATCAGAGATTACACGCAGAG
  TTCTTCATCGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201568 representing NM_017012
  Red=Cloning site Green=Tags(s)
  
  MVLLLILSVLLLKEDVRGSAQSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVE
  AMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSFRSKK
  PIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNW
  TYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVACFCEGM
  TVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNLR
  NPWFQEFWQHRFQCRLEGFAQENSKYNKTCNSSLTLRTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPG
  YAGLCDAMKPIDGRKLLDSLMKTNFTGVSGDMILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGE
  LKMDDDEVWSKKNNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDD
  LTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVIFIIYRDTPVVKSSSRELCYIILAGICLGYLC
  TFCLIAKPKQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILI
  CIQLGIIVALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEA
  KYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTST
  VVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSSNGKSVTWAQNEKSTRGQHLWQRLSVHINKKENP
  NQTAVIKPFPKSTENRGPGAAAGGGSGPGVAGAGNAGCTATGGPEPPDAGPKALYDVAEAEESFPAAARP
  RSPSPISTLSHLAGSAGRTDDDAPSLHSETAARSSSSQGSLMEQISSVVTRFTANISELNSMMLSTAATP
  GPPGTPICSSYLIPKEIQLPTTMTTFAEIQPLPAIEVTGGAQGATGVSPAQETPTGAESAPGKPDLEELV
  ALTPPSPFRDSVDSGSTTPNSPVSESALCIPSSPKYDTLIIRDYTQSSSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017012
• ORF Size: 3513 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017012.1, NP_058708.1
• RefSeq Size: 3918 bp
• RefSeq ORF: 3516 bp
• Locus ID: 24418
• UniProt ID: P31424
• Cytogenetics: 1q32
• MW: 128.3 kDa
• Gene Summary: may play a role in synaptic transmission; may interact with mGluR1, another group I metabotropic glutamate receptor [RGD, Feb 2006]