Cdc27 (NM_001024793) Rat Tagged ORF Clone

CAT#: RR201505

Cdc27 (Myc-DDK-tagged ORF) - Rat cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10 ug)

Product Images

Circular map for RR201505

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR201505 representing NM_001024793
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGACG
  CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTCTACTGGCGACCTG
  TTACTACCGCTCAGGAAAGGCCTATAAAGCCTATAGACTCTTGAAAGGACACAGTTGTACCACCCCGCAG
  TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAAATCTTATCTG
  GTGGAGTGTTTAATAAGCAAAAAAGCCATGACGACCTTGTCACTGAGTTTGGTGATTCAGCTTGCTTCAC
  TCTTTCCTTGTTGGGACATGTATATTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAG
  AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
  CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACGTGTACAGCTCT
  AGTATCTAACCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACTCCCCAAGACACGATT
  GAATTAAACAGACTGAACTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
  ACATTGATTCAGCTGTAATTTCACCAGATAACGTCCCCCTTGGAACTGGTCCTTCCATATTATCTAAACA
  GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
  AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
  CACCTTCTGTAATTGACGTGCCATCCACCGGAGCACCTACTAAAAAGTCCGTTGCAAGAATGGGCCACAC
  TGGAGCGAAGTCTGTCTTCTCACAGAGTGGGAACAGTCGGGAGGCCACACCGGTGCTCGTTGCCCAGACA
  CAAAGCTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATTACATCTCCCCCAAATG
  CATTGCCTCGAAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAAGGAGAATAGCAAAAAGTT
  AAAAATGAAGTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGAATAACT
  CAACCCAACATAAACGATAGTCTGGAAATTACAAAATTGGACTCTTCTATCATTTCAGAAGGGAAAATAT
  CCACAATCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGGTTTGATGAGCCTTCTTCG
  TGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATAAATATTCTGAGCCAC
  CTACCTTCTCACCACTACAGTACTGGCTGGGTCCTGTGCCAGATTGGACGGGCTTATTTTGAACTTTCAG
  AATACATGCAGGCTGAAAGAATATTCTCAGAAGTTAGAAGGATTGAGAGTTTCAGAGTTGAGGGTATGGA
  GATCTACTCTACAACACTCTGGCATCTTCAGAAAGATGTTGCTCTTTCGGTTCTTTCAAAAGATTTAACA
  GACATGGATAAGAATTCACCAGAGGCTTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGGGAGCATG
  ATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTGGATCCAAATTATGCTTATGCCTATACTCTATT
  AGGACATGAATTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTATAAGAGTA
  AATCCCAGACATTATAATGCATGGTATGGTTTAGGGATGATTTATTACAAGCAAGAGAAATTCAGCCTCG
  CAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTTACTTTGCCACATTGGAGT
  AGTTCAGCATGCACTCAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTTATAGATCCC
  AAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAAAATACAAGTCTGCTTTAC
  AAGAACTTGAAGAACTGAAACAGATTGTCCCCAAAGAATCCCTCGTTTACTTCTTAATAGGAAAGGTTTA
  CAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAAGGA
  GCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGAGGAGCCGATAACCCAGG
  AGGAGCAGATCATGGGAACAGATGAATCCCAGGAGAGCAGCATGACAGATGCAGATGACACTCAACTTCA
  TGCAGCGGAAAGTGATGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR201505 representing NM_001024793
  Red=Cloning site Green=Tags(s)
  
  MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQ
  CKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDLVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQK
  SLSLNPFLWSPFESLCEIGEKPDPDQTFKLTSLQNFSSCLPNTCTALVSNHSLSHRQPETVLTETPQDTI
  ELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDNVPLGTGPSILSKQVQNKPKTGRSLLGGPTALSPLTP
  SFGILPLETPSPGDGSYLQNYTNTPSVIDVPSTGAPTKKSVARMGHTGAKSVFSQSGNSREATPVLVAQT
  QSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGIT
  QPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSH
  LPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIYSTTLWHLQKDVALSVLSKDLT
  DMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRV
  NPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDP
  KNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKG
  ANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024793
• ORF Size: 2472 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024793.1, NP_001019964.1
• RefSeq Size: 3045 bp
• RefSeq ORF: 2475 bp
• Locus ID: 360643
• UniProt ID: Q4V8A2
• Cytogenetics: 10q32.1
• MW: 91.7 kDa
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]