Polr1c (NM_001008330) Rat Tagged ORF Clone

CAT#: RR200906

Polr1c (Myc-DDK-tagged ORF) - Rat polymerase (RNA) I polypeptide C (Polr1c), (10 ug)

Product Images

Circular map for RR200906

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Polr1c
• Synonyms: Ac2-127; Rpo1-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200906 representing NM_001008330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCTCAGGCGGTGGAGGAAATGCGGACTCGCGTGGTTCTGGGAGAGTTTGGGGTTCGCAATG
  TCCACACCACTGACTTTCCCGGTAACTACTCCGGTTATGATGATGCCTGGGACCAGGACCGGTTCGAGAA
  GAACTTCCGTGTGGACGTGGTGCATATGGACGAGAGCACACTGGAGTTCGACATGGTGGGGATTGATGCT
  GCCATTGCCAATGCCTTCCGGAGGATTCTGTTAGCTGAGGTGCCAACAATGGCTGTGGAAAAGGTTTTGG
  TGTATAACAACACATCCATCGTCCAGGATGAGATCCTTGCTCACCGCCTGGGGCTTATCCCCATTCTTGC
  TGATCCACGTCTCTTTGAATATCGGAACCCAGGAGAGGAGGAGGGAACAGAGATAGATACACTACAGTTT
  CGGCTGCAGGTCAGGTGTACCAGGAACCCCAGTGCTGCTAAGGATTCTTCCGACCCCAATGAACTCTATG
  TGAACCACAAAGTTTATACCAGGCACATGACGTGGGTGCCCTTGGGAAACCAGGCTGATGTCTTCCCGGA
  GGGCACGGTTCGCCCTGTGCATGACGACATCCTCATTGCCCAGCTTCGGCCCGGCCAGGAGATTGACCTG
  ATGATGCACTGCGTCAAGGGCATCGGCAAAGACCATGCCAAGTTCTCACCGGTGGCCACAGCCAGCTACA
  GGCTCCTGCCGGACATCACCCTGCTTGAACCTGTAGAAGGGGAAGCAGCCGAGGAGCTGAGCCAGTGCTT
  CTCACCCGGCGTGATTGCGGTGCAGGAAGTACAAGGTAAAAAGGTGGCCAGGGTTGCCAATGCTCGGCTG
  GATACCTTCAGCAGGGAGATCTTCCGTCATGAGAAGCTCAAGAAGGCCGTGCGGCTTGCCCGTGTTCGGG
  ACCATTACATCTTTTCTGTGGAGTCAACTGGGGTTTTGCCACCAGATGTGTTGGTGAGTGAAGCTATCAA
  GATCCTGATGGGGAAGTGCCGGCGGTTCTTGGATGAACTAGATGCAGTTGAGATGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200906 representing NM_001008330
  Red=Cloning site Green=Tags(s)
  
  MAAAQAVEEMRTRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDESTLEFDMVGIDA
  AIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPILADPRLFEYRNPGEEEGTEIDTLQF
  RLQVRCTRNPSAAKDSSDPNELYVNHKVYTRHMTWVPLGNQADVFPEGTVRPVHDDILIAQLRPGQEIDL
  MMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSQCFSPGVIAVQEVQGKKVARVANARL
  DTFSREIFRHEKLKKAVRLARVRDHYIFSVESTGVLPPDVLVSEAIKILMGKCRRFLDELDAVEMD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008330
• ORF Size: 1038 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008330.1, NP_001008331.1
• RefSeq Size: 1320 bp
• RefSeq ORF: 1041 bp
• Locus ID: 301246
• Cytogenetics: 9q12
• MW: 39 kDa