Dnmt1 (NM_053354) Rat Tagged ORF Clone

CAT#: RR200671

Dnmt1 (Myc-DDK-tagged ORF) - Rat DNA (cytosine-5-)-methyltransferase 1 (Dnmt1), (10 ug)

Product Images

Circular map for RR200671

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dnmt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200671 representing NM_053354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCACGAACAGCTCCAGCCCGAGTGCCTGCACTTGCCTCCCCGGCAGGCTCGCTCCCCGACCATG
  TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAAAGAGAAATTGAA
  CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGCTATGCGACTTGGAAACCAAACTACATAAA
  GAGGAATTGTCTGAGGAAGGCTACCTGGCTAAAGTCAAGACCCTTCTAAATAAGGATTTGTGCCTGGAGA
  ACGGAACACTCTCTCTCACTCAGAAAGCCAACGGTTGTCCTGCCAACGGGAGTCGGCCAACCTGGAAAGC
  AGAAATGGCAGACTCAAACCGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGGAGCAAGTCG
  GACAGTGAGACCATGATTGAAGCTTCATCTAGTTCGGTGGCTACGAGGAGAACCACCAGGCAGACCACCA
  TCACGTCTCACTTCAAGGGTCCCGCTAAACGGAAACCCAAGGAAGACTCTGAAAAGGGAAACGCAAATGA
  ATCTGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTCGCAGGCACAGAGAGTCGTGCCAGTCGT
  GCCGGAGAGTCTGTGGAGAAACCTGAACGAGTAAGGCCAGGAACCCAGCTGTGTCAGGAAGAGCAAGGTG
  AACAGGAAGATGACAGGAGACCTCGACGTCAAACCAGAGAGCTAGCATCAAGGCGGAAATCAAGGGAGGA
  CCCAGACAGAGAAGCAAGGCCAGGAACTCACTTGGATGTGGACGATGACGATGAAAAGGACAAAAGAAGT
  TCCAGGCCCAGAAGCCAGCCCAGAGATCTAGCCACCAAACGGAGACCCAAGGAAGAAGTAGAGCAGATCA
  CTCCAGAGCCTCCCGAGGGCAAAGATGAGGACGAAAGGGAGGAGAAGAGACGAAAAACGACACGTAAAAA
  GCCGGAACCACTCAGCATTCCCGTACAGAGCAGAGTGGAGAGAAAAGCCTCTCAGGGCAAAGCAAGTGCA
  ATCCCAAAGCTCAACCCGCCACAGTGCCCTGAGTGTGGCCAGTACCTAGATGACCCTGACCTGAAGTACC
  AGCAGCACCCTGTGGATGCTGTGGATGAACCCCAGATGCTGACCAATGAGGCACTGTCCGTCTTTGACTC
  CAACTCGTCTTGGTTTGAGACCTATGATTCCTCTCCCATGCATAAGTTCACTTTCTTCAGTGTGTATTGC
  AGTCGCGGTCACTTGTGTCCTGTCGACACTGGTCTCATTGAGAAGAACGTTGAACTCTACTTTTCTGGTG
  TTGCCAAAGCAATTCATGAAGAAAATCCGTCTGTGGAAGGTGGTGTTAATGGCAAGAACCTGGGGCCAAT
  CAATCAGTGGTGGATCAGTGGCTTTGACGGTGGCGAGAAGGCGCTCATTGGCTTTTCTACCGCATTTGCT
  GAATACTTTCTGATGGAGCCCAGCCCAGAGTATGCACCAATATTTGGGCTGATGCAGGAGAAAATTTACA
  TCAGCAAGATTGTGGTCGAGTTCTTGCAGAGCAATCCTGACGCTGTCTATGAAGACTTGATCAATAAGAT
  CGAGACCACTGTTCCTCCTTCTGCCATCAATGTGAACCGGTTCACCGAGGACTCCCTCCTACGCCATGCC
  CAGTTTGTGGTGAGCCAGGTAGAAAGTTACGACGACGCTAAGGACGATGATGAGACGCCCATCTTCTTGT
  CTCCCTGTATGAGGTCCCTGATCCATTTGGCTGGTGTCTCGCTGGGACAGAGGCGAGCAACAAGGCGCAC
  GGTCATCAATTCTGCCAAAGTGAAGCGCAAGGGGCCCACAAAAGCCACCACCACCAAGCTGGTCTATCAG
  ATCTTCGACACTTTCTTCTCGGAGCAGATCGAGAAGGATGACAAGGAGGACAAAGAGAACACCATGAAGC
  GCCGCCGGTGTGGTGTCTGTGAGGTCTGTCAACAGCCTGAGTGTGGGAAGTGCAAGGCGTGCAAAGACAT
  GGTTAAGTTTGGTGGCACCGGACGGAGTAAACAGGCTTGCCTCAAGAGGAGGTGTCCTAACTTGGCCGTA
  AAGGAGGCAGATGAAGACGAAGAAGCTGATGATGATATACCAGAGTTGCCGTCACCCAAAAAGTTGCACC
  AAGGGAAGAAGAAGAAACAGAATAAGGATCGCATCTCCTGGCTCGGGGAGCCTGTGAAGATCGAAGAGAA
  CAGAACTTACTATTGGAAGGTGAGCATCGACGAAGAGACTCTGGAGGTGGGCGACTGCGTCTCGGTCATT
  CCGGATGATCCCTCCAAACCACTGTATCTAGCCAGGGTCACAGCACTGTGGGAAGACAAGAACGGGCAGA
  TGTTCCATGCACACTGGTTCTGCGCGGGGACAGACACGGTCCTCGGAGCCACCTCTGACCCTCTGGAGCT
  GTTCCTTGTAGGCGAGTGTGAAAACATGCAACTTTCCTACATCCACAGCAAGGTCAAGGTCATCTACAGA
  GGGCCTTCCCCAAACTGGGCCATGGAGGGAGGCATGGACCCTGAGGCCATGTTGCCGGGGGCTGAGGATG
  GGAAGACCTACTTCTACCAGTTCTGGTACAGCCAGGACTACGCAAGGTTTGAGTCCCCACCCAAGACCCA
  GCCGACGGAGGACAATAAGCACAAGTTCTGCCTATCTTGTATCCGGCTGGCTGAGCTGAGACAAAAAGAA
  ATGCCCAAAGTCCTGGAACAACTTGAGGAGGTGGACGGCCGGGTCTACTGCAGTTCTATCACCAAGAACG
  GTGTTGTCTACCGACTGGGTGACAGTGTGTACCTGCCTCCTGAGGCCTTTACTTTCAACATCAAAATGGC
  CAGCCCCATGAAACGCTCTAAGAGGGACCCTGTGAATGAGAACCTGTACCCCGAGCATTACCGCAAGTAT
  TCCGACTACATCAAAGGAAGCAACCTGGACGCCCCTGAACCCTATCGGATTGGTCGGATAAAAGAGATCC
  ACTGTGGCAAGAAGAAAGGTGGCAAGGTCAATGAGGCAGACATCAAGATCAGGCTCTACAAGTTCTATAG
  GCCTGAGAACACCCACAAGTCCATCCAGGCAACCTACCACGCCGACATCAACCTGCTCTACTGGAGCGAT
  GAGGAAGCCGTGGTGGACTTCAGCGACGTGCAAGGCCGCTGCACCGTGGAGTACGGAGAGGACCTGCTTG
  AGAGCATCCAGGATTACTCACAGGGCGGCCCCGACCGCTTCTACTTTCTCGAGGCCTACAATTCAAAGAC
  TAAAAGCTTTGAAGACCCTCCGAACCATGCCCGCAGCCCTGGGAACAAAGGCAAAGGAAAGGGGAAAGGG
  AAAGGGAAGGGAAAACCTCAGGTATCAGAGCCCAAAGAGCCCGAGGCAGCCATCAAACTGCCTAAGCTGC
  GGACCCTGGATGTGTTTTCTGGCTGTGGAGGATTAACAGAAGGATTCCACCAAGCAGGCATCTCGGAGAC
  GCTGTGGGCCATCGAGATGTGGGAACCAGCAGCCCAGGCATTTCGGCTGAACAACCCTGGCACCACGGTG
  TTCACAGAGGACTGCAACGTGCTTCTCAAGCTGGTCATGGCTGGTGAGGTGACCAACTCTCTGGGCCAGA
  GGCTGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCGCCCTGCCAGGGCTTCAGTGGCATGAA
  CCGTTTCAACTCTCGTACTTACTCCAAGTTCAAAAACTCCCTCGTGGTCTCCTTCCTCAGCTACTGTGAC
  TACTACCGGCCTCGGTTCTTCCTCTTGGAGAATGTCAGGAACTTCGTGTCCTTCAGGCGCTCCATGGTGC
  TGAAGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTCGGTGTGCTCCAGGCTGGACA
  GTATGGTGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCTGGAGAGAAGCTGCCTCTG
  TTCCCAGAACCTCTACACGTGTTTGCCCCCCGTGCCTGCCAGCTGAGTGTTGTGGTGGACGACAAGAAGT
  TCGTTAGCAACATTACAAGGCTAAGCTCGGGTCCCTTCCGAACCATCACGGTGCGAGATACCATGTCTGA
  CCTCCCTGAGATCCAGAATGGAGCCTCAGCCCCCGAGATCTCCTACAATGGAGAGCCACAGTCCTGGTTC
  CAGAGGCAGCTGCGAGGGTCGCACTACCAGCCCATCCTCAGGGACCATATCTGCAAGGACATGAGCGCGC
  TGGTGGCTGCCCGTATGCGGCACATCCCGCTGTCCCCAGGATCGGATTGGCGTGACCTGCCCAACATACA
  AGTGCGGCTGAGAGATGGCGTCATAACCAATAAACTTCGCTACACTTTCCACGACACGAAGAACGGCTGC
  AGCAGCACCGGGGCCCTGCGTGGGGTCTGCTCCTGTGCGGAAGGCAAGACCTGCGACCCTGCTTCCAGAC
  AATTCAACACGCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAACCACTGGGCTGGCCTCTA
  CGGGCGTCTGGAGTGGGATGGCTTCTTCAGTACCACCGTCACCAACCCTGAGCCCATGGGCAAGCAAGGC
  CGGGTGCTCCATCCCGAGCAGCACCGGGTCGTGAGTGTGCGGGAATGTGCTCGCTCCCAAGGCTTTCCAG
  ATACCTACCGGTTATTCGGCAACATCCTGGACAGACACCGGCAGGTGGGGAACGCTGTGCCACCACCACT
  GGCCAAAGCCATCGGCCTGGAGATTAAGCTCTGTCTGCTGGCCAGCGCTCAGGAGAGTGCCTCAGCTGCA
  GTTAAAGGAAAGGAGGAGACTACTACCGAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200671 representing NM_053354
  Red=Cloning site Green=Tags(s)
  
  MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVKEKLNLLHEFLQTEIKSQLCDLETKLHK
  EELSEEGYLAKVKTLLNKDLCLENGTLSLTQKANGCPANGSRPTWKAEMADSNRSPRSRPKPRGPRRSKS
  DSETMIEASSSSVATRRTTRQTTITSHFKGPAKRKPKEDSEKGNANESAAEERDQDKKRRVAGTESRASR
  AGESVEKPERVRPGTQLCQEEQGEQEDDRRPRRQTRELASRRKSREDPDREARPGTHLDVDDDDEKDKRS
  SRPRSQPRDLATKRRPKEEVEQITPEPPEGKDEDEREEKRRKTTRKKPEPLSIPVQSRVERKASQGKASA
  IPKLNPPQCPECGQYLDDPDLKYQQHPVDAVDEPQMLTNEALSVFDSNSSWFETYDSSPMHKFTFFSVYC
  SRGHLCPVDTGLIEKNVELYFSGVAKAIHEENPSVEGGVNGKNLGPINQWWISGFDGGEKALIGFSTAFA
  EYFLMEPSPEYAPIFGLMQEKIYISKIVVEFLQSNPDAVYEDLINKIETTVPPSAINVNRFTEDSLLRHA
  QFVVSQVESYDDAKDDDETPIFLSPCMRSLIHLAGVSLGQRRATRRTVINSAKVKRKGPTKATTTKLVYQ
  IFDTFFSEQIEKDDKEDKENTMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAV
  KEADEDEEADDDIPELPSPKKLHQGKKKKQNKDRISWLGEPVKIEENRTYYWKVSIDEETLEVGDCVSVI
  PDDPSKPLYLARVTALWEDKNGQMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYR
  GPSPNWAMEGGMDPEAMLPGAEDGKTYFYQFWYSQDYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKE
  MPKVLEQLEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKMASPMKRSKRDPVNENLYPEHYRKY
  SDYIKGSNLDAPEPYRIGRIKEIHCGKKKGGKVNEADIKIRLYKFYRPENTHKSIQATYHADINLLYWSD
  EEAVVDFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKSFEDPPNHARSPGNKGKGKGKG
  KGKGKPQVSEPKEPEAAIKLPKLRTLDVFSGCGGLTEGFHQAGISETLWAIEMWEPAAQAFRLNNPGTTV
  FTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCD
  YYRPRFFLLENVRNFVSFRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPL
  FPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASAPEISYNGEPQSWF
  QRQLRGSHYQPILRDHICKDMSALVAARMRHIPLSPGSDWRDLPNIQVRLRDGVITNKLRYTFHDTKNGC
  SSTGALRGVCSCAEGKTCDPASRQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQG
  RVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLASAQESASAA
  VKGKEETTTED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053354
• ORF Size: 4863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053354.3, NP_445806.3
• RefSeq Size: 5917 bp
• RefSeq ORF: 4866 bp
• Locus ID: 84350
• Cytogenetics: 8q13
• MW: 183 kDa
• Gene Summary: methylates genomic DNA; involved in processes of gene inactivation, chromatin organization, X chromosome inactivation and genomic imprinting [RGD, Feb 2006]