Camk2b (NM_001042354) Rat Tagged ORF Clone

CAT#: RR200521

Camk2b (Myc-DDK-tagged ORF) - Rat calcium/calmodulin-dependent protein kinase II beta (Camk2b), transcript variant 1, (10 ug)

Product Images

Circular map for RR200521

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Camk2b
• Synonyms: Ck2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200521 representing NM_001042354
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCACGGTGACCTGCACCCGTTTCACGGACGAGTACCAGCTATACGAGGATATTGGCAAGGGGG
  CTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGCACCGGCCATGAGTATGCAGCTAAGATCATTAACAC
  CAAGAAGCTGTCAGCTAGAGATCACCAGAAGCTGGAGAGGGAGGCTCGGATCTGCCGCCTGCTGAAGCAT
  TCCAACATTGTACGCCTCCATGACAGCATCTCTGAAGAGGGCTTCCACTACCTGGTCTTCGACCTGGTCA
  CTGGTGGGGAGCTCTTTGAAGACATTGTGGCGAGAGAGTACTACAGTGAGGCTGACGCCAGTCACTGTAT
  CCAGCAGATCCTGGAGGCTGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCTGAA
  AACCTGCTCCTGGCCAGCAAATGCAAAGGGGCCGCAGTGAAACTGGCAGACTTCGGCCTGGCCATCGAGG
  TTCAGGGAGACCAGCAGGCATGGTTTGGATTTGCGGGAACACCAGGCTACCTGTCTCCCGAAGTTCTTCG
  GAAGGAGGCCTATGGCAAACCAGTGGATATCTGGGCATGTGGGGTGATCCTGTATATCCTGCTGGTGGGA
  TACCCACCTTTCTGGGATGAGGACCAGCACAAGCTGTACCAGCAGATCAAGGCTGGGGCCTATGACTTCC
  CATCCCCCGAGTGGGACACCGTTACCCCTGAAGCCAAAAACCTCATCAACCAGATGTTGACCATCAACCC
  CGCCAAGCGCATCACGGCCCACGAGGCCCTGAAGCACCCATGGGTCTGCCAACGATCCACGGTGGCCTCC
  ATGATGCACAGACAGGAGACTGTGGAATGTCTGAAGAAGTTCAATGCAAGGAGGAAGCTCAAGGGAGCCA
  TCCTCACCACTATGCTGGCCACACGGAATTTCTCAGCAGCCAAGAGTTTACTCAACAAGAAAGCAGACGG
  AGTCAAGCCCCAGACAAACAGCACCAAAAACAGCTCGGCCATCACCAGCCCCAAAGGATCCCTCCCTCCT
  GCCGCCCTGGAATCTTCCGACAGCACCAACACAACCATAGAGGACGAAGATGCCAAAGCCCCCAGGATCT
  CTGACATCCTGAACTCAGTGAGAAGGGGCTGTGGAACCCCAGAAGCCGAGGGACCCCTATCAGTGGGGCC
  CCCGCCCTGCTTGTCTCCGGGTCTCCTAGGCCCCCTGCCCACCCCGTCCCCCAGGATCTCTGACATCCTC
  AACTCAGTGAGGCGGGGCTCAGGGACCCCAGAAGCTGAGGGCCTCCCACCAGTGGGCCCCCCACCCTGCC
  CATCTCCGACTCTCCCTGGCCCCTTGCCCACCCCATCCCGGAAGCAGGAAATCATCAAGACCACAGAGCA
  GCTCATCGAGGCCGTCAACAACGGCGACTTTGAGGCCTATGCGAAAATCTGTGACCCAGGCCTGACCTCA
  TTTGAGCCTGAAGCTCTGGGCAACCTGGTCGAAGGGATGGATTTCCACAGATTCTACTTTGAGAACCTGC
  TGGCCAAGAACAGCAAGCCGATCCACACCACTATCCTGAACCCGCACGTGCACGTCATCGGCGAGGATGC
  AGCCTGCATCGCTTACATCCGCCTCACACAGTACATCGACGGCCAGGGCAGACCCCGCACCAGCCAGTCC
  GAAGAGACCCGTGTGTGGCACCGCCGCGACGGCAAGTGGCAGAATGTCCATTTCCACTGCTCGGGCGCTC
  CAGTGGCCCCACTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200521 representing NM_001042354
  Red=Cloning site Green=Tags(s)
  
  MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKH
  SNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPE
  NLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVG
  YPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVAS
  MMHRQETVECLKKFNARRKLKGAILTTMLATRNFSAAKSLLNKKADGVKPQTNSTKNSSAITSPKGSLPP
  AALESSDSTNTTIEDEDAKAPRISDILNSVRRGCGTPEAEGPLSVGPPPCLSPGLLGPLPTPSPRISDIL
  NSVRRGSGTPEAEGLPPVGPPPCPSPTLPGPLPTPSRKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTS
  FEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQS
  EETRVWHRRDGKWQNVHFHCSGAPVAPLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042354
• ORF Size: 1767 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 4005 bp
• RefSeq ORF: 1770 bp
• Locus ID: 24245
• Cytogenetics: 14q21
• MW: 65 kDa
• Gene Summary: beta subunit of calcium/calmodulin-dependent protein kinase II; may be involved in retinal development [RGD, Feb 2006]