Cast (NM_001033716) Rat Tagged ORF Clone

CAT#: RR200418

Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 3, (10 ug)

Product Images

Circular map for RR200418

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cast
• Synonyms: MGC108997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200418 representing NM_001033716
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC
  AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT
  GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA
  GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG
  AAGGGTCAGAGACAAAATTTCAAGATGCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGT
  GGCCACTGCCTCAGACAAGGTGGTTGTTAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACC
  TTAGGTGAATGTGAAGATACTAACAAAGATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGG
  ATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGA
  GAAAAATGAAGCTATCACAGGACCTCTTCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGAT
  GCCTTGTCATCTGATTTCACCTGTAGCTCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGG
  AAAGTTCGAAAGCTCAGTCTGCAGGAGTGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGT
  AGAAGAGGAGGTCATGAATGATCAAGCCCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGAC
  CCTCAGAGCCATCTTAGGCAAGCCAAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAAT
  GTGGTGAAGATGAGGACACAGTACCTGCTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACC
  ACTATTGCCAGAACCTGAAGAAACATCTAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCA
  GATTTTGTCCAACCTACATATCAAGAAAAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGC
  CAGATGATGCTGTAGAAACCTTGGCTAGAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATC
  TCTGGTGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACA
  ATTCCTCCTGATTATCGACTAGAAATAGTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCG
  AGGAACAACTTCCACCCTTAAGCGATGACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGC
  AAACATATTGTCTCTTGGTTTTGAAGATGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTG
  CCTGCTCCAAGCAACCACACAGCAGCCCCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATG
  CCTTGGATGAACTTTCTGACAGTCTTGGACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGA
  TAAAGTAAAGGAAAAAATCAAAGCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCT
  GAATATAGGCATCTCCTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGA
  AGCCTGGTCAGGACCAGGACCCCATTGATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGA
  GACTTCACAGAATACAACAAAGGAGAAGGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAG
  AAAACAAAGGATTCCTCCAAGAAGACAGAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200418 representing NM_001033716
  Red=Cloning site Green=Tags(s)
  
  MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKI
  ESEKSQSSEPPVIHEKKPKGKPKEGSETKFQDAPSADGESVAGGVTVATASDKVVVKSGVNAALDDLIDT
  LGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLEKNEAITGPLPDSPKPMGIDHAID
  ALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKVEEEVMNDQALQALSDSLGTRQPD
  PQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKCLSESELIGELSA
  DFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKSLVDKVKEKAKEEDHEKLGEKEET
  IPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPANILSLGFEDAKLSAAVSETVSQV
  PAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDDKVKEKIKAEHSEKLGERDDTIPP
  EYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTETSQNTTKEKGKKTSSSKASKNEE
  KTKDSSKKTEEVPKPKVDEDAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033716
• ORF Size: 1956 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033716.1, NP_001028888.1
• RefSeq Size: 4466 bp
• RefSeq ORF: 1959 bp
• Locus ID: 25403
• UniProt ID: P27321
• Cytogenetics: 2q11
• MW: 70.6 kDa
• Gene Summary: inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006]