Cast (NM_001033715) Rat Tagged ORF Clone

CAT#: RR200417

Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 2, (10 ug)

Product Images

Circular map for RR200417

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cast
• Synonyms: MGC108997
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200417 representing NM_001033715
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC
  AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT
  GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA
  GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG
  AAGGGTCAGAGACAAAATTTCAAGATGCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGT
  GGCCACTGCCTCAGACAAGGTGGTTGTTAAGAAAAAAGAGAAGAAATCATTAACACCTACTTTACCTATG
  GAGTCCACACTAAACAAGCTATCTGACAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACCT
  TAGGTGAATGTGAAGATACTAACAAAGATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGGA
  TTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGAG
  AAAAATGAAGCTATCACAGGACCTCTTCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGATG
  CCTTGTCATCTGATTTCACCTGTAGCTCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGGA
  AAGTTCGAAAGCTCAGTCTGCAGGAGTGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGTA
  GAAGAGGAGGTCATGAATGATCAAGCCCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGACC
  CTCAGAGCCATCTTAGGCAAGCCAAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAATG
  TGGTGAAGATGAGGACACAGTACCTGCTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACCA
  CTATTGCCAGAACCTGAAGAAACATCTAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCAG
  ATTTTGTCCAACCTACATATCAAGAAAAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGCC
  AGATGATGCTGTAGAAACCTTGGCTAGAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATCT
  CTGGTGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACAA
  TTCCTCCTGATTATCGACTAGAAATAGTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCGA
  GGAACAACTTCCACCCTTAAGCGATGACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGCA
  AACATATTGTCTCTTGGTTTTGAAGATGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTGC
  CTGCTCCAAGCAACCACACAGCAGCCCCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATGC
  CTTGGATGAACTTTCTGACAGTCTTGGACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGAT
  AAAGTAAAGGAAAAAATCAAAGCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTG
  AATATAGGCATCTCCTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGAA
  GCCTGGTCAGGACCAGGACCCCATTGATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGAG
  ACTTCACAGAATACAACAAAGGAGAAGGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAGA
  AAACAAAGGATTCCTCCAAGAAGACAGAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200417 representing NM_001033715
  Red=Cloning site Green=Tags(s)
  
  MSRPGPKPAASSRPRRGAAASHTQEHVNEKNIGSSSKPAEKKGSDEVTASSAATGTSPRMSTTGAKAVKI
  ESEKSQSSEPPVIHEKKPKGKPKEGSETKFQDAPSADGESVAGGVTVATASDKVVVKKKEKKSLTPTLPM
  ESTLNKLSDKSGVNAALDDLIDTLGECEDTNKDDPPYTGPVVLDPMDSTYLEALGIKEGTIPPEYRKLLE
  KNEAITGPLPDSPKPMGIDHAIDALSSDFTCSSPTGKQTEKEKSTGESSKAQSAGVTRSAVPPQEKKRKV
  EEEVMNDQALQALSDSLGTRQPDPQSHLRQAKQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKP
  LLPEPEETSKCLSESELIGELSADFVQPTYQEKPSMPAAKIKKGVVPDDAVETLARSLGTRKEDPEDEKS
  LVDKVKEKAKEEDHEKLGEKEETIPPDYRLEIVKDKDGKPLLPKEAEEQLPPLSDDFLLDALSQDFSSPA
  NILSLGFEDAKLSAAVSETVSQVPAPSNHTAAPPPGTERRDKELDDALDELSDSLGQRQPDPDENKPLDD
  KVKEKIKAEHSEKLGERDDTIPPEYRHLLDNDGKDKPEKPLTKNTEKPGQDQDPIDALSEDLDSCPPTTE
  TSQNTTKEKGKKTSSSKASKNEEKTKDSSKKTEEVPKPKVDEDAT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033715
• ORF Size: 2025 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033715.1, NP_001028887.1
• RefSeq Size: 4535 bp
• RefSeq ORF: 2028 bp
• Locus ID: 25403
• UniProt ID: P27321
• Cytogenetics: 2q11
• MW: 73.2 kDa
• Gene Summary: inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006]