Il1rl1 (NM_013037) Rat Tagged ORF Clone

CAT#: RR200411

Il1rl1 (Myc-DDK-tagged ORF) - Rat interleukin 1 receptor-like 1 (Il1rl1), transcript variant 1, (10 ug)

Product Images

Circular map for RR200411

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Il1rl1
• Synonyms: Fit-1; FIT1; St2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200411 representing NM_013037
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGGCAAATGGAGAATGGGGCTTTGGGCTTTGGCAATTCTGACAGTTCCCATGTATTTCATAGTGA
  CAGAGGGCAGAAAAACATCCTGGGGTCTAGAAAACGAGGCTTTAATTGTCAGATGCCCCCAAAGAGGAGG
  TGCGATTAACCCTGTGGAATGGTATTATTCAAATACAAATGAAAGAATTCCTACTCAAAAGAGAAATCGG
  ATCTTCGTCTCAAGAGATCGTCTGAAGTTTCTACCAGCCAAAGTGGAAGACTCTGGGATTTATACGTGTG
  TTATCAGAAGCCCTGAATCGATTAAGACCGGATCTTTGAATGTCACCATATATAAAAGACCACCAAACTG
  CAAAATCCCTGATTACATGATGTACTCGACAGTAGATGGATCAGATAAAAATTCCAAGATAACATGTCCA
  ACAATTGCCTTGTATAATTGGACAGCGCCTGTTCAGTGGTTTAAGAACTGCAAAGCTCTCCAAGGGCCAA
  GGTTCAGGGCACACATGTCCTATTTGTTCATTGACAAAGTGAGTCATGTTGATGAAGGTGACTACACATG
  TCGATTCACTCACACGGAGAACGGAACCAATTACATTGTGACTGCCACCAGATCATTCACAGTTGAAGAA
  AAAGGCTTCTCTACATTTCCAGTAATTACAAACCCTCCACACAACTACACAGTGGAAGTGGAAATAGGAA
  AAACAGCAAACATTGCCTGCTCAGCTTGCTTTGGCACAGCCTCTCAGTTCGTTGCTGTCCTGTGGCAGAT
  TAACAAAACGAGAATTGGATCTTTTGGCAAAGCAAGAATTCAAGAAGAGAAAGGCCCAAATAAAAGTTCC
  AGCAATGGCATGATTTGCTTAACCTCACTGTTAAGGATAACTGGTGTGACCGACAAGGACTTCTCCCTGA
  AATATGACTGTGTGGCCATGAACCATCACGGAGTGATAAGGCACCCCGTAAGACTGAGAAGGAAACAACC
  AAGTAAGGAGTGTCTCTCACAAATTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200411 representing NM_013037
  Red=Cloning site Green=Tags(s)
  
  MIGKWRMGLWALAILTVPMYFIVTEGRKTSWGLENEALIVRCPQRGGAINPVEWYYSNTNERIPTQKRNR
  IFVSRDRLKFLPAKVEDSGIYTCVIRSPESIKTGSLNVTIYKRPPNCKIPDYMMYSTVDGSDKNSKITCP
  TIALYNWTAPVQWFKNCKALQGPRFRAHMSYLFIDKVSHVDEGDYTCRFTHTENGTNYIVTATRSFTVEE
  KGFSTFPVITNPPHNYTVEVEIGKTANIACSACFGTASQFVAVLWQINKTRIGSFGKARIQEEKGPNKSS
  SNGMICLTSLLRITGVTDKDFSLKYDCVAMNHHGVIRHPVRLRRKQPSKECLSQIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013037
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013037.1, NP_037169.1
• RefSeq Size: 2586 bp
• RefSeq ORF: 1011 bp
• Locus ID: 25556
• UniProt ID: Q62611
• Cytogenetics: 9q22
• MW: 38.1 kDa
• Gene Summary: may be involved in modulating IL-1 signaling [RGD, Feb 2006]