Prom1 (NM_021751) Rat Tagged ORF Clone

CAT#: RR200326

Prom1 (Myc-DDK-tagged ORF) - Rat prominin 1 (Prom1), transcript variant 1, (10 ug)

Product Images

Circular map for RR200326

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Prom1
• Synonyms: CD133; Prom
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200326 representing NM_021751
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTCGTATTCAGTGTCCTGCTGCTACTGGGGCTGTGTGGAAAGATGGCTTCAGGAGGTCAGCCTG
  CATTCGATAACACTCCCGGGGCTTTGAATTATGAATTGCCTACCACAGAATATGAGACCCAAGACACCTT
  CAATGCTGGGATTATTGACCCTCTCTACCAAATGGTGCACATCTTCCTCAACGTGGTCCAGCCGAATGAC
  TTCCCTCAAGATTTGGTCAAAAAACTCATACAGAAGAGGTTTGACATCTCGGTTGATACCAAGGAGGTCG
  CCATCTATGAAATTGGAGTTCTTATCTGCGTCATCCTGGGCCTGCTGTTCATTTTCCTCATGCCCCTGGT
  GGGCTTCTTCTTTTGTATGTGCCGTTGCTGTAACAAATGCGGCGGAGAAATGCACCAGCGGCAGAAGCAG
  AACGAGTCGTGCCGGAGGAAGTGCTTAGCCATCTCCCTCTTGCTGATTTGTCTGCTCATGAGTCTTGGCA
  TCGCGTTTGGCTTTGTGGCCAACCAGCAGACAAGGACTCGGATCCAAAGGACCCAGAAGCTGGCAGAGAG
  CAATTACAGAGACTTGCGGGCTCTCCTGACTGAAGCCCCAAAGCAAATCGACTATATTCTGGGGCAATAC
  AACACAACCAAGAACAAGGCATTCTCAGACCTGGACAGTATCGATTCCGTGCTGGGAGGCAGAATAAAGG
  GCCAACTAAAACCCAAAGTTACTCCTGTTCTCGAAGAGATTAAGGCCATGGCAACAGCCATTAGACAGAC
  CAAGGATGCCCTGCAGAACATGAGCAGCAGTCTGAAAAGTCTACGTGACGCAAGCACCCAGCTCAGTACC
  AACCTGACGTCTGTGAGAAACAGCATCGAGAATTCACTCAACAGCAACGACTGTGCTTCAGACCCAGCCA
  GCAAGATCTGTGATAGCCTCAGACCACAGCTGAGCAATCTGGGGAGCAACCACAACGGAAGTCAGCTCCC
  ATCAGTGGACAGGGAACTCAACACCGTTAATGATGTCGACAGAACCGATCTGGAGAGCCTCGTCAAAAGG
  GGGTATATGTCGATTGATGAAATACCCAATATGATACAAAACCAAACTGGGGATGTCATCAAAGATGTCA
  AAAAGACCTTGGATTCTGTTAGCTCCAAGGTTAAGAACATGAGCCAAAGTATTCCTGTCGAGGAGGTGCT
  GCTTCAGTTCTCCCACTACCTTAATGACAGCAACAGGTACATCCACGAGTCGCTGCCCAGAGTGGAAGAA
  TATGACTCATACTGGTGGCTGGGTGGCTTAATAGTCTGCTTCCTGCTGACCCTCATTGTGACTTTCTTTT
  ACCTGGGCTTGCTGTGTGGTGTGTTTGGCTATGACAAGCGTGCCACCCCAACCAGAAGAGGCTGCGTGTC
  CAACACTGGAGGCATCTTCCTCATGGCTGGGGTTGGATTCAGCTTCCTTTTTTGCTGGATATTGATGATC
  CTTGTGGTTCTTACTTTTGTTGTTGGTGCAAATGTGGAAAAGTTGCTCTGTGAACCTTATGAAAACAAGA
  AATTACTACAGGTTTTGGATACTCCCTACCTGCTCAACGATCAATGGCAATTCTATCTCTCTGGCATACT
  ACTCAAAAACCCAGACATTAACATGACCTTTGAGCAAGTCTACAGGGATTGCAAAAGAGGTCGAGGAGTG
  TATGCCACTTTTCAGCTTGAGAATGTCTTCAACATCACTGAGAATTTCAACATTGAGAGGCTTTCTGAAG
  ACATAGTGAAGGAGTTGGAAAAGCTGAATGTGAACATTGACAGCATCGAACTGTTGGACAAAACAGGAAG
  GAAGAGCCTCGAGGACTTTGCACAATCTGGGATAGATAGAATCAATTATTCCATGTACTTACAGGAGGCT
  GAGAAACCCCCTACCAAAGTGGACCTGCTGACATTTGCCTCTTTTCTGGAAACAGAAGCAAACCAGTTGC
  CTGACGGAAATCTGAAACAGGCCTTCTTAATGGATGCACAGAACATTCGAGCCATCCACCAGCAGCACGT
  CCCTCCTGTGCAGCAGTCACTGAATTCATTAAAACAAAGTGTCTGGGCCCTTAAGCAAACAAGCAGCAAG
  TTGCCGGAGGAAGTGAAGAAGGTCCTTGCCTCTTTGGATTCTGCTCAGCATTTCCTCACCAGTAACCTCT
  CCTCCATCGTTATTGGAGAAACAAAGAAGTTTGGGAGAACAATAATAGGCTACTTTGAACATTATCTGCA
  ATGGGTCTTGTACGCCATCACAGAGAAGATGACATCCTGCAAACCCATGATTACAGCAATGGACTCTGCT
  GTTAATGGCATTCTGTGTAGCTATGTTGCTGACCCTCTGAATCTGTTCTGGTTCGGCATAGGGAAAGCCA
  CCATGCTCTTACTTCCGGCTGTAATCATCGCTATCAAGTTGGCCAAGTACTATCGCAGGATGGATTCAGA
  GGATGTATATGATGATGTTGAGACTGTACCCATGAAAAATTTGGAAAACGGTAGTAATGGTTATCATAAA
  GATCATTTATATGGTGTTCACAATCCTGTTATGACAAGCCCGTCTCGATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200326 representing NM_021751
  Red=Cloning site Green=Tags(s)
  
  MALVFSVLLLLGLCGKMASGGQPAFDNTPGALNYELPTTEYETQDTFNAGIIDPLYQMVHIFLNVVQPND
  FPQDLVKKLIQKRFDISVDTKEVAIYEIGVLICVILGLLFIFLMPLVGFFFCMCRCCNKCGGEMHQRQKQ
  NESCRRKCLAISLLLICLLMSLGIAFGFVANQQTRTRIQRTQKLAESNYRDLRALLTEAPKQIDYILGQY
  NTTKNKAFSDLDSIDSVLGGRIKGQLKPKVTPVLEEIKAMATAIRQTKDALQNMSSSLKSLRDASTQLST
  NLTSVRNSIENSLNSNDCASDPASKICDSLRPQLSNLGSNHNGSQLPSVDRELNTVNDVDRTDLESLVKR
  GYMSIDEIPNMIQNQTGDVIKDVKKTLDSVSSKVKNMSQSIPVEEVLLQFSHYLNDSNRYIHESLPRVEE
  YDSYWWLGGLIVCFLLTLIVTFFYLGLLCGVFGYDKRATPTRRGCVSNTGGIFLMAGVGFSFLFCWILMI
  LVVLTFVVGANVEKLLCEPYENKKLLQVLDTPYLLNDQWQFYLSGILLKNPDINMTFEQVYRDCKRGRGV
  YATFQLENVFNITENFNIERLSEDIVKELEKLNVNIDSIELLDKTGRKSLEDFAQSGIDRINYSMYLQEA
  EKPPTKVDLLTFASFLETEANQLPDGNLKQAFLMDAQNIRAIHQQHVPPVQQSLNSLKQSVWALKQTSSK
  LPEEVKKVLASLDSAQHFLTSNLSSIVIGETKKFGRTIIGYFEHYLQWVLYAITEKMTSCKPMITAMDSA
  VNGILCSYVADPLNLFWFGIGKATMLLLPAVIIAIKLAKYYRRMDSEDVYDDVETVPMKNLENGSNGYHK
  DHLYGVHNPVMTSPSRY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021751
• ORF Size: 2571 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021751.2, NP_068519.2
• RefSeq Size: 3714 bp
• RefSeq ORF: 2574 bp
• Locus ID: 60357
• Cytogenetics: 14q21
• MW: 96.6 kDa
• Gene Summary: induces expression of 3-phosphate glyceraldehyde dehydrogenase (GAPDH); may play a role in glucose homeostasis [RGD, Feb 2006]