Hip1r (NM_031234) Rat Tagged ORF Clone

CAT#: RR200269

Hip1r (Myc-DDK-tagged ORF) - Rat huntingtin interacting protein 1 related (Hip1r), transcript variant 2, (10 ug)

Product Images

Circular map for RR200269

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hip1r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200269 representing NM_031234
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCATCAAGAATGTGCCGGCGCGGGTGCTGAGCCGCAGGCCGGGCCATAGCCTAGAGGCCGAGC
  GCGAGCAGTTCGACAAGACGCAGGCCATCAGTATCAGCAAAGCCATCAACAGCCAGGAGGCCCCAGTGAA
  GGAGAAGCATGCCCGGCGTATCATTCTGGGCACTCACCATGAGAAAGGAGCCTTTACCTTCTGGTCCTAT
  GCCATCGGCCTGCCGCTGCCCAGCAGCTCCATCCTCAGTTGGAAATTCTGTCACGTCCTTCACAAAGTCC
  TCCGAGATGGACACCCCAATGTGCTGCATGACTGCCAGCGGTACCGGAGCAACATACGTGAGATTGGCGA
  CTTGTGGGGCCACCTACGTGACCAGTATGGACACCTGGTGAATATCTATACCAAACTGCTGCTGACTAAA
  ATCTCCTTCCACCTTAAGCACCCCCAGTTTCCTGCGGGCTTGGAGGTAACAGATGAAGTGTTGGAGAAGG
  CGGCGGGAACTGATGTTAATAACATTTTTCAGCTCACTGTGGAGATGTTTGACTACATGGACTGTGAACT
  GAAGCTTTCTGAGTCAGTTTTCCGGCAGCTCAACACAGCCATCGCAGTGTCACAGATGTCTTCTGGCCAG
  TGTCGCCTGGCGCCGCTCATCCAGGTCATCCAGGACTGCAGCCACCTGTACCACTACACGGTGAAGCTCA
  TGTTCAAGCTCCACTCCTGTCTCCCAGCAGACACCCTGCAAGGCCACAGGGATCGGTTCCACGAGCAGTT
  TCACAGTCTCAGAAACTTCTTCCGCCGAGCCTCGGACATGCTCTACTTCAAGCGACTCATCCAGATCCCC
  AGACTGCCTGAGGGACCCCCCAATTTCCTGCGGGCCTCAGCCCTGGCTGAGCACATCAAGCCGGTGGTGG
  TGATTCCAGAAGAGGCCCCGGAGGAGGAGGAGCCTGAGAACCTAATTGAGATCAGCAGTGGGCCCCCTGC
  TGGGGAGCCAGTGGTGGTGGCTGACCTCTTTGATCAGACCTTTGGACCCCCCAATGGCTCCATGAAGGAT
  GACAGGGACCTCCAGATTGAGAACTTGAAGAGAGAGGTGGAGACCCTCCGTGCTGAGCTGGAGAAGATTA
  AGATGGAGGCCCAGCGGTACATCTCGCAGCTGAAGGGCCAGGTGAACAGCCTGGAGGCAGAGCTGGAGGA
  GCAGCGGAAGCAGAAGCAGAAGGCCCTGGTGGACAACGAGCAGCTGCGCCACGAGCTGGCCCAGCTGAAA
  GCCCTGCAGCTGGAAGGCGCGCGCAACCAGGGCCTGCGAGAGGAGGCGGAGAGGAAGGCCAGTGCCACTG
  AGGCACGCTACAGCAAGCTGAAGGAGAAGCACAATGAACTCATTAACACGCATGCCGAGCTGCTCAGGAA
  GAATGCAGACACAGCCAAGCAGCTGACGGTGACACAGCAGAGCCAGGAGGAGGTGGCACGGGTAAAGGAG
  CAGCTGGCCTTCCAGATGGAGCAAGTGAAGCGGGAGTCTGAGATGAAGATGGAGGAGCAGAGTGACCAGC
  TGGAGAAGCTCAAGAGGGAGCTGGTGGCCAAGGCAGGAGAGCTGGCCCATGCCCAGGAGGCCCTGAGCCG
  CACGGAACAGAGTGGGTCAGAGCTGAGCTCACGGCTAGATACACTGAACGCAGAGAAGGAAGCACTGAGC
  GGAGCCATTCGGCAGCGTGAGGCAGAGCTGCTGGCTGCCCAGAGCCTGGTGCGGGAGAAGGAAGAGGCTC
  TTAGCCAAGAGCAGCAGCGCAGCGCCCAGGAGAAGGGCGAGCTACAGGGGCGGCTGGCAGAAAAGGAGTC
  TCAGGAGCAGGGGCTCCAGCAGAAGCTGCTGGATGAACAGTTTGCAGTGCTGCGAGGTGCAGCCGCCGAG
  GCGGAGGCCATCCTGCAGGACGCAGTGAGCAAGCTGGATGACCCCCTGCACCTCCGTTGCACCAGCTCCC
  CAGACTACTTAGTGAGCCGGGCTCAGGCAGCCCTGGACAGCGTGAGTGGCCTGGAGAAGGGCCACACCCA
  GTACCTGGCTTCCTCTGAGGATGCTTCCGCCCTGGTAGCAGCACTGACCCGCTTCTCCCATCTGGCCGCG
  GACACCATTGTCAACGGTGGCGCCACCTCGCACCTGGCCCCCACTGACCCCGCTGACCGCCTGATCGACA
  CGTGCAGGGAGTGTGGAGCCCGGGCTCTGGAGCTCATGGGACAGCTGCAGGACCAGACAAAGCTACCGAG
  GGCCCAGCCCAGCCTGATGCGGGCCCCTCTGCAGGGCATTCTTCAGTTGGGCCAGGACCTGAAGCCTAAG
  AGCCTGGACGTCCGGCAAGAGGAGCTAGGGGCCATGGTTGACAAGGAGATGGCAGCCACCTCTGCAGCCA
  TTGAGGACGCTGTGCGGAGGATCGAGGACATGATGAACCAGGCCCGCCACGAGAGCTCAGGGGTGAAACT
  AGAGGTGAACGAGAGGATCCTCAACTCCTGCACAGATCTGATGAAGGCCATCCGGCTCCTGGTGATGACG
  TCCACCAGCCTGCAGAAGGAGATTGTGGAGAGCGGCAGGGGGGCAGCAACACAGCAGGAATTTTATGCCA
  AGAATTCACGGTGGACTGAAGGCCTCATCTCTGCCTCGAAGGCAGTGGGCTGGGGAGCCACACAGCTGGT
  GGAGTCAGCTGACAAGGTTGTGCTTCACATGGGCAAATATGAGGAACTCATCGTCTGCTCACACGAGATT
  GCAGCCAGCACTGCCCAGCTGGTGGCAGCCTCGAAGGTAAAAGCCAACAAGAACAGTCCCCACCTGAGCC
  GCCTGCAGGAGTGTTCCCGCACTGTCAACGAGAGGGCTGCCAATGTGGTGGCCTCCACCAAGTCTGGCCA
  GGAGCAGATCGAGGACAGAGACACCATGGATTTCTCTGGCCTGTCTCTCATCAAGCTGAAGAAGCAGGAG
  ATGGAGACACAGGTGCGAGTCTTGGAGCTGGAGAAGACGCTAGAGGCAGAGCGCGTACGGCTCGGGGAGC
  TTCGGAAGCAGCACTATGTACTGGCGGGGGGCATGGGGACACCTGGCGAAGAAGAACCCAGCAGACCCAG
  CCCAGCTCCCCGAAGTGGGGCCACTAAGAAGCCACCTCTTGCCCAGAAACCCAGCATAGCCCCCAGGCCG
  GACAACCAGCTCGACAAGAAGGATGGTGTCTACCCAGCTCAACTTGTGAACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200269 representing NM_031234
  Red=Cloning site Green=Tags(s)
  
  MNSIKNVPARVLSRRPGHSLEAEREQFDKTQAISISKAINSQEAPVKEKHARRIILGTHHEKGAFTFWSY
  AIGLPLPSSSILSWKFCHVLHKVLRDGHPNVLHDCQRYRSNIREIGDLWGHLRDQYGHLVNIYTKLLLTK
  ISFHLKHPQFPAGLEVTDEVLEKAAGTDVNNIFQLTVEMFDYMDCELKLSESVFRQLNTAIAVSQMSSGQ
  CRLAPLIQVIQDCSHLYHYTVKLMFKLHSCLPADTLQGHRDRFHEQFHSLRNFFRRASDMLYFKRLIQIP
  RLPEGPPNFLRASALAEHIKPVVVIPEEAPEEEEPENLIEISSGPPAGEPVVVADLFDQTFGPPNGSMKD
  DRDLQIENLKREVETLRAELEKIKMEAQRYISQLKGQVNSLEAELEEQRKQKQKALVDNEQLRHELAQLK
  ALQLEGARNQGLREEAERKASATEARYSKLKEKHNELINTHAELLRKNADTAKQLTVTQQSQEEVARVKE
  QLAFQMEQVKRESEMKMEEQSDQLEKLKRELVAKAGELAHAQEALSRTEQSGSELSSRLDTLNAEKEALS
  GAIRQREAELLAAQSLVREKEEALSQEQQRSAQEKGELQGRLAEKESQEQGLQQKLLDEQFAVLRGAAAE
  AEAILQDAVSKLDDPLHLRCTSSPDYLVSRAQAALDSVSGLEKGHTQYLASSEDASALVAALTRFSHLAA
  DTIVNGGATSHLAPTDPADRLIDTCRECGARALELMGQLQDQTKLPRAQPSLMRAPLQGILQLGQDLKPK
  SLDVRQEELGAMVDKEMAATSAAIEDAVRRIEDMMNQARHESSGVKLEVNERILNSCTDLMKAIRLLVMT
  STSLQKEIVESGRGAATQQEFYAKNSRWTEGLISASKAVGWGATQLVESADKVVLHMGKYEELIVCSHEI
  AASTAQLVAASKVKANKNSPHLSRLQECSRTVNERAANVVASTKSGQEQIEDRDTMDFSGLSLIKLKKQE
  METQVRVLELEKTLEAERVRLGELRKQHYVLAGGMGTPGEEEPSRPSPAPRSGATKKPPLAQKPSIAPRP
  DNQLDKKDGVYPAQLVNY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031234
• ORF Size: 3204 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031234.1, NP_112513.1
• RefSeq Size: 4354 bp
• RefSeq ORF: 3207 bp
• Locus ID: 81917
• Cytogenetics: 12q15
• MW: 119.4 kDa
• Gene Summary: may interact with F-actin, huntingtin, and huntingtin interacting protein HIP1; may play a role in vesicular mediated transport [RGD, Feb 2006]