Hip1r (NM_001134763) Rat Tagged ORF Clone

CAT#: RR200268

Hip1r (Myc-DDK-tagged ORF) - Rat huntingtin interacting protein 1 related (Hip1r), transcript variant 1, (10 ug)

Product Images

Circular map for RR200268

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hip1r
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200268 representing NM_001134763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGCATCAAGAATGTGCCGGCGCGGGTGCTGAGCCGCAGGCCGGGCCATAGCCTAGAGGCCGAGC
  GCGAGCAGTTCGACAAGACGCAGGCCATCAGTATCAGCAAAGCCATCAACAGCCAGGAGGCCCCAGTGAA
  GGAGAAGCATGCCCGGCGTATCATTCTGGGCACTCACCATGAGAAAGGAGCCTTTACCTTCTGGTCCTAT
  GCCATCGGCCTGCCGCTGCCCAGCAGCTCCATCCTCAGTTGGAAATTCTGTCACGTCCTTCACAAAGTCC
  TCCGAGATGGACACCCCAATGTGCTGCATGACTGCCAGCGGTACCGGAGCAACATACGTGAGATTGGCGA
  CTTGTGGGGCCACCTACGTGACCAGTATGGACACCTGGTGAATATCTATACCAAACTGCTGCTGACTAAA
  ATCTCCTTCCACCTTAAGCACCCCCAGTTTCCTGCGGGCTTGGAGGTAACAGATGAAGTGTTGGAGAAGG
  CGGCGGGAACTGATGTTAATAACATTTTTCAGCTCACTGTGGAGATGTTTGACTACATGGACTGTGAACT
  GAAGCTTTCTGAGTCAGTTTTCCGGCAGCTCAACACAGCCATCGCAGTGTCACAGATGTCTTCTGGCCAG
  TGTCGCCTGGCGCCGCTCATCCAGGTCATCCAGGACTGCAGCCACCTGTACCACTACACGGTGAAGCTCA
  TGTTCAAGCTCCACTCCTGTCTCCCAGCAGACACCCTGCAAGGCCACAGGGATCGGTTCCACGAGCAGTT
  TCACAGTCTCAGAAACTTCTTCCGCCGAGCCTCGGACATGCTCTACTTCAAGCGACTCATCCAGATCCCC
  AGACTGCCTGAGGGACCCCCCAATTTCCTGCGGGCCTCAGCCCTGGCTGAGCACATCAAGCCGGTGGTGG
  TGATTCCAGAAGAGGCCCCGGAGGAGGAGGAGCCTGAGAACCTAATTGAGATCAGCAGTGGGCCCCCTGC
  TGGGGAGCCAGTGCAGGTGGTGGCTGACCTCTTTGATCAGACCTTTGGACCCCCCAATGGCTCCATGAAG
  GATGACAGGGACCTCCAGATTGAGAACTTGAAGAGAGAGGTGGAGACCCTCCGTGCTGAGCTGGAGAAGA
  TTAAGATGGAGGCCCAGCGGTACATCTCGCAGCTGAAGGGCCAGGTGAACAGCCTGGAGGCAGAGCTGGA
  GGAGCAGCGGAAGCAGAAGCAGAAGGCCCTGGTGGACAACGAGCAGCTGCGCCACGAGCTGGCCCAGCTG
  AAAGCCCTGCAGCTGGAAGGCGCGCGCAACCAGGGCCTGCGAGAGGAGGCGGAGAGGAAGGCCAGTGCCA
  CTGAGGCACGCTACAGCAAGCTGAAGGAGAAGCACAATGAACTCATTAACACGCATGCCGAGCTGCTCAG
  GAAGAATGCAGACACAGCCAAGCAGCTGACGGTGACACAGCAGAGCCAGGAGGAGGTGGCACGGGTAAAG
  GAGCAGCTGGCCTTCCAGATGGAGCAAGTGAAGCGGGAGTCTGAGATGAAGATGGAGGAGCAGAGTGACC
  AGCTGGAGAAGCTCAAGAGGGAGCTGGTGGCCAAGGCAGGAGAGCTGGCCCATGCCCAGGAGGCCCTGAG
  CCGCACGGAACAGAGTGGGTCAGAGCTGAGCTCACGGCTAGATACACTGAACGCAGAGAAGGAAGCACTG
  AGCGGAGCCATTCGGCAGCGTGAGGCAGAGCTGCTGGCTGCCCAGAGCCTGGTGCGGGAGAAGGAAGAGG
  CTCTTAGCCAAGAGCAGCAGCGCAGCGCCCAGGAGAAGGGCGAGCTACAGGGGCGGCTGGCAGAAAAGGA
  GTCTCAGGAGCAGGGGCTCCAGCAGAAGCTGCTGGATGAACAGTTTGCAGTGCTGCGAGGTGCAGCCGCC
  GAGGCGGAGGCCATCCTGCAGGACGCAGTGAGCAAGCTGGATGACCCCCTGCACCTCCGTTGCACCAGCT
  CCCCAGACTACTTAGTGAGCCGGGCTCAGGCAGCCCTGGACAGCGTGAGTGGCCTGGAGAAGGGCCACAC
  CCAGTACCTGGCTTCCTCTGAGGATGCTTCCGCCCTGGTAGCAGCACTGACCCGCTTCTCCCATCTGGCC
  GCGGACACCATTGTCAACGGTGGCGCCACCTCGCACCTGGCCCCCACTGACCCCGCTGACCGCCTGATCG
  ACACGTGCAGGGAGTGTGGAGCCCGGGCTCTGGAGCTCATGGGACAGCTGCAGGACCAGACAAAGCTACC
  GAGGGCCCAGCCCAGCCTGATGCGGGCCCCTCTGCAGGGCATTCTTCAGTTGGGCCAGGACCTGAAGCCT
  AAGAGCCTGGACGTCCGGCAAGAGGAGCTAGGGGCCATGGTTGACAAGGAGATGGCAGCCACCTCTGCAG
  CCATTGAGGACGCTGTGCGGAGGATCGAGGACATGATGAACCAGGCCCGCCACGAGAGCTCAGGGGTGAA
  ACTAGAGGTGAACGAGAGGATCCTCAACTCCTGCACAGATCTGATGAAGGCCATCCGGCTCCTGGTGATG
  ACGTCCACCAGCCTGCAGAAGGAGATTGTGGAGAGCGGCAGGGGGGCAGCAACACAGCAGGAATTTTATG
  CCAAGAATTCACGGTGGACTGAAGGCCTCATCTCTGCCTCGAAGGCAGTGGGCTGGGGAGCCACACAGCT
  GGTGGAGTCAGCTGACAAGGTTGTGCTTCACATGGGCAAATATGAGGAACTCATCGTCTGCTCACACGAG
  ATTGCAGCCAGCACTGCCCAGCTGGTGGCAGCCTCGAAGGTAAAAGCCAACAAGAACAGTCCCCACCTGA
  GCCGCCTGCAGGAGTGTTCCCGCACTGTCAACGAGAGGGCTGCCAATGTGGTGGCCTCCACCAAGTCTGG
  CCAGGAGCAGATCGAGGACAGAGACACCATGGATTTCTCTGGCCTGTCTCTCATCAAGCTGAAGAAGCAG
  GAGATGGAGACACAGGTGCGAGTCTTGGAGCTGGAGAAGACGCTAGAGGCAGAGCGCGTACGGCTCGGGG
  AGCTTCGGAAGCAGCACTATGTACTGGCGGGGGGCATGGGGACACCTGGCGAAGAAGAACCCAGCAGACC
  CAGCCCAGCTCCCCGAAGTGGGGCCACTAAGAAGCCACCTCTTGCCCAGAAACCCAGCATAGCCCCCAGG
  CCGGACAACCAGCTCGACAAGAAGGATGGTGTCTACCCAGCTCAACTTGTGAACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200268 representing NM_001134763
  Red=Cloning site Green=Tags(s)
  
  MNSIKNVPARVLSRRPGHSLEAEREQFDKTQAISISKAINSQEAPVKEKHARRIILGTHHEKGAFTFWSY
  AIGLPLPSSSILSWKFCHVLHKVLRDGHPNVLHDCQRYRSNIREIGDLWGHLRDQYGHLVNIYTKLLLTK
  ISFHLKHPQFPAGLEVTDEVLEKAAGTDVNNIFQLTVEMFDYMDCELKLSESVFRQLNTAIAVSQMSSGQ
  CRLAPLIQVIQDCSHLYHYTVKLMFKLHSCLPADTLQGHRDRFHEQFHSLRNFFRRASDMLYFKRLIQIP
  RLPEGPPNFLRASALAEHIKPVVVIPEEAPEEEEPENLIEISSGPPAGEPVQVVADLFDQTFGPPNGSMK
  DDRDLQIENLKREVETLRAELEKIKMEAQRYISQLKGQVNSLEAELEEQRKQKQKALVDNEQLRHELAQL
  KALQLEGARNQGLREEAERKASATEARYSKLKEKHNELINTHAELLRKNADTAKQLTVTQQSQEEVARVK
  EQLAFQMEQVKRESEMKMEEQSDQLEKLKRELVAKAGELAHAQEALSRTEQSGSELSSRLDTLNAEKEAL
  SGAIRQREAELLAAQSLVREKEEALSQEQQRSAQEKGELQGRLAEKESQEQGLQQKLLDEQFAVLRGAAA
  EAEAILQDAVSKLDDPLHLRCTSSPDYLVSRAQAALDSVSGLEKGHTQYLASSEDASALVAALTRFSHLA
  ADTIVNGGATSHLAPTDPADRLIDTCRECGARALELMGQLQDQTKLPRAQPSLMRAPLQGILQLGQDLKP
  KSLDVRQEELGAMVDKEMAATSAAIEDAVRRIEDMMNQARHESSGVKLEVNERILNSCTDLMKAIRLLVM
  TSTSLQKEIVESGRGAATQQEFYAKNSRWTEGLISASKAVGWGATQLVESADKVVLHMGKYEELIVCSHE
  IAASTAQLVAASKVKANKNSPHLSRLQECSRTVNERAANVVASTKSGQEQIEDRDTMDFSGLSLIKLKKQ
  EMETQVRVLELEKTLEAERVRLGELRKQHYVLAGGMGTPGEEEPSRPSPAPRSGATKKPPLAQKPSIAPR
  PDNQLDKKDGVYPAQLVNY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134763
• ORF Size: 3207 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134763.1, NP_001128235.1
• RefSeq Size: 4357 bp
• RefSeq ORF: 3210 bp
• Locus ID: 81917
• Cytogenetics: 12q15
• MW: 119.6 kDa
• Gene Summary: may interact with F-actin, huntingtin, and huntingtin interacting protein HIP1; may play a role in vesicular mediated transport [RGD, Feb 2006]