Akap12 (NM_001033653) Rat Tagged ORF Clone

CAT#: RR200267

Akap12 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 12 (Akap12), transcript variant 2, (10 ug)

Product Images

Circular map for RR200267

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akap12
• Synonyms: AKAP12A; AKAP12B; AKAP12G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200267 representing NM_001033653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGACCATTACCATCACAGTTGGACAGCGAGAGTCAGAAGATGTGAGAGAAAAAGACCGAGTTG
  AAGAAATGGCGGCCAACTCCACAGCTGTTGAAGATATCACAAAGGATGGGCAGGAGGAGACATCAGAAAT
  AATTGAACAGATCCCTGCTTCAGAAAGCAATGTGGAAGAAATGGTACAGCCTGCTGAGTCCCAGGCTAAT
  GATGTTGGCTTCAAGAAAGTATTTAAATTTGTTGGTTTTAAATTCACGGTGAAGAAGGATAAAAATGAAA
  AGTCAGATACTGTCCAACTACTCACTGTCAAGAAGGATGAAGGCGAAGGGGCAGAAGCCTCTGTCGGAGC
  TGGAGACCACCAGGAGCCCAGTGTGGAGACTGCCGTCGGAGAGTCAGCATCCAAAGAAAGTGAGCTGAAG
  CAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGAACAGAGCAGCACAGAAATCCCCCTTCAAGCCG
  AATCTGATCAAGCGGCTGAGGAAGAAGCCAAAGATGAAGGAGAAGAAAAACAAGAGAAAGAGCCCACCAA
  GTCCCCAGAATCCCCGAGCAGCCCAGTCAGCAGTGAGACAACATCTTCCTTCAAGAAGTTCTTCACTCAC
  GGTTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAATCAAAAGAGGATGATCTGGAAACTGCCGAGA
  AGAGAAAGGAGCAAGAGGCAGAAAAAGTAGACGAGGAAGAAAAGGAAAAGACAGAGCCAGCCTCGGAGGA
  GCAGGAGCCGGCAGAAGACACAGACCAGGCCAGGTTGTCAGCAGACTACGAGAAGGTGGAGCTGCCTTTG
  GAAGACCAGGTTGGTGACCTGGAGGCATCGTCAGAGGAGAAGTGTGCTCCTTTGGCAACGGAAGTGTTTG
  ATGAGAAGATGGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCACGTGAGCACCGTGGAGAAGACAGAGGA
  GGAGCAGGGAGGAGGAGGAGAGGCTGAAGGGGGCGTGGTGGTAGAAGGAACAGGAGAATCCTTGCCCCCT
  GAGAAACTGGCTGAGCCCCAGGAGGTCCCCCAGGAAGCTGAGCCTGCTGAGGAGCTGATGAAGAGCAGAG
  AGATGTGTGTCTCTGGAGGAGACCACACTCAACTGACAGACCTAAGTCCTGAAGAGAAGACGCTGCCCAA
  ACACCCAGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAGGAAAGAATCAAGGTACAGGGAAGT
  CCCTTGAAGAAACTCTTCAGTAGCTCAGGCTTAAAGAAGCTGTCTGGGAAGAAGCAGAAGGGGAAACGAG
  GAGGTGGGGGAGACGAAGAGCCTGGAGAATACCAACACATTCACACCGAATCCCCAGAGAGTGCTGATGA
  GCAGAAGGGAGAGAGCTCTGCGTCGTCCCCCGAGGAGCCTGAGGAGACCACGTGTCTGGAGAAAGGGCCG
  CTGGAAGCACCCCAGGATGGGGAAGCTGAGGAAGGAACTACTTCCGATGGAGAGAAGAAGAGGGAAGGGA
  TCACTCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAAGAAACGGGTCCGAAGACCTTCTGAGAGTGA
  CAAGGAGGAAGAGCTGGAGAAGGTCAAGAGCGCCACCTTGTCCTCCACTGATAGCACAGTGTCAGAAATG
  CAAGATGAAGTCAAAACTGTTGGTGAGGAACAAAAGCCAGAGGAACCAAAGCGTAGGGTGGATACTTCAG
  TGTCTTGGGAAGCACTGATTTGTGTCGGATCATCCAAGAAGAGAGCAAGGAAGGCATCCTCTTCAGATGA
  TGAAGGAGGGCCAAGGACACTGGGAGGGGACAGTCACAGAGCAGAGGAGGCCAGCAAAGACAAAGAAGCC
  GGAACAGACGCTGTTCCTGCCAGCACCCAGGAGCAGGACCAAGCGCAAGGAAGTTCCTCACCCGAGCCAG
  CGGGAAGCCCTTCCGAAGGGGAAGGTGTCTCCACTTGGGAGTCATTTAAAAGATTAGTCACTCCAAGAAA
  AAAATCCAAGTCAAAACTGGAAGAGAAAGCCGAAGACTCTAGTGTAGAGCAGTTGTCCACTGAGATCGAA
  CCGAGTAGAGAAGAATCTTGGGTTTCCATTAAGAAATTCATCCCCGGACGGCGGAAGAAAAGGGCAGATG
  GGAAGCAAGAACAAGCCACTGTGGAAGACTCAGGGCCAGTGGAGATAAATGAGGACGACCCTGATGTCCC
  AGCCGTCGTGCCTCTGTCTGAGTATGATGCAGTGGAGAGGGAGAAGATGGAAGCCCAGGGGAATGCGGAG
  CTGCCCCAGCTGCTGGGGGCTGTGTACGTGTCCGAGGAGCTCAGTAAGACTCTGGTCCACACTGTGAGTG
  TCGCAGTCATTGATGGGACCAGGGCAGTCACCAGTGTCGAAGAGCGGTCTCCTTCGTGGATATCCGCTTC
  CGTAACAGAACCTCTTGAACACACAGCGGGAGAAGCCATGCCACCTGTTGAAGAGGTCACTGAAAAAGAC
  ATCATTGCAGAAGAAACTCCTGTGCTCACCCAGACGTTACCAGAGGGTAAAGATGCCCATGACGACATGG
  TCACCAGTGAAGTGGATTTCACCTCAGAAGCTGTGACAGCCACAGAGACCTCAGAGGCTCTCCGTACTGA
  AGAAGTTACCGAAGCATCGGGGGCCGAAGAGACCACAGACATGGTGTCCGCAGTTTCCCAGCTGACTGAC
  TCCCCAGACACCACAGAGGAAGCCACCCCAGTTCAGGAGGTAGAGGGTGGTGTGCTAGATACAGAAGAAG
  AGGAGCGCCAGACGCAGGCCATCCTCCAAGCCGTTGCAGACAAGGTGAAAGAGGAGTCCCAGGTGCCTGC
  AACCCAGACTGTGCAGAGAACGGGGTCAAAAGCACTGGAGAAGGTTGAGGAGGTAGAGGAGGACTCCGAA
  GTGCTGGCTTCGGAGAAAGAGAAGGACGTTATGCCGAAAGGACCCGTGCAGGAAGCTGGAGCTGAGCATC
  TTGCACAGGGCTCTGAGACTGGACAGGCTACTCCAGAGAGCCTTGAAGTTCCTGAAGTCACAGCAGATGT
  AGACCATGTCGCCACGTGCCAGGTTATCAAGCTCCAGCAGCTGATGGAACAGGCCGTGGCCCCTGAGTCA
  TCCGAAACCTTGACAGACAGTGAGACAAATGGAAGCACTCCCTTAGCAGATTCAGACACTGCAGATGGGA
  CACAGCAAGATGAAACCATTGACAGCCAGGACAGTAAAGCCACTGCAGCTGTCAGGCAGTCACAGGTCAC
  AGAAGAAGAGGCGGCTACTGCTCAGAAAGAGGAGCCTTCGACACTACCTAATAATGTTCCAGCCCAGGAA
  GAACATGGGGAAGAACCAGGAAGAGATGTTCTTGAACCTACACAGCAAGAGCTTGCTGCTGCAGCCGTGC
  CCGTTCTGGCAAAGACTGAGGTGGGTCAAGAGGGTGAGGTTGACTGGTTGGATGGAGAAAAAGTCAAAGA
  AGAACAGGAGGTGTTTGTACACTCTGGACCCAACAGTCAAAAGGCTGCTGATGTGACATATGACAGTGAA
  GTGATGGGAGTGGCCGGGTGTCAGGAAAAGGAGAGTACTGAAGTGCAGAGTCTTAGCCTGGAGGAGGGAG
  AGATGGAAACTGACGTTGAAAAGGAGAAAAGGGAGACAAAGCCAGAGCAAGTGAGTGAAGAAGGTGAGCA
  GGAAACAGCCGCTCCTGAGCATGAAGGAACCTACGGGAAGCCAGTCCTGACACTTGACATGCCCAGCTCA
  GAGAGGGGGAAGGCACTGGGAAGCCTTGGAGGAAGCCCTTCTCTCCCAGACCAAGACAAAGCAGGTTGCA
  TAGAGGTTCAAGTTCAAAGCCTGGACACAACAGTCACTCAAACAGCAGAAGCTGTGGAAAAGGTCATAGA
  AACGGTTGTGATTTCAGAGACAGGTGAAAGTCCAGAGTGTGTAGGTGCACACTTATTACCAGCTGAGAAG
  TCCTCTGCAACGGGTGGCCACTGGACTCTTCAGCATGCAGAGGACACGGTACCCCTGGGGCCTGAGTCTC
  AGGCAGAATCCATCCCAATCATAGTAACTCCTGCTCCTGAAAGCACCCTACATCCTGACCTACAAGGAGA
  AATAAGCGCATCCCAGAGAGAGCGATCAGAGGAAGAGGACAAGCCAGATGCTGGTCCTGATGCTGACGGC
  AAGGAGAGTACAGCAATCGAAAAAGTCCTCAAGGCTGAACCTGAGATCCTGGAACTTGAGAGTAAGAGCA
  ACAAGATTGTGCTGAACGTCATTCAGACAGCCGTTGACCAGTTCGCACGTACAGAAACAGCCCCCGAAAC
  TCATGCTTATGATTCACAGACCCAGGTTCCTGCAATGCAGGCTGACAGCCAGGGAGCCCAACAGATGCTG
  GACAAAAATGAAAGTTGCCAAGATGAAACACCCAGTGCCGCAGCCCAGAGAGGACTTGCAAGTCCTGACC
  GTTCTGGAGGCATGGGCTCAGCCTCGGAAATGCTTGCCGCGCTTGCAGTTGAAAGCGCCGGTGTCAAAGT
  AAGCATTGAGAAGCTGCCTCCTCAACCCAAAGATCAAAAGGAGCATGCTGCTGATGGCCCTCAGCTCCAA
  AGCTTAGCCCAGGCAGAGGCCAGTGCCTCTGGAAACCTAACCAAAGAATCCCCAGACACCAACGGACCAA
  AGCTAACCGAGGAGGGCGATGCCCCAAAAGTTGAGGTCCAGGAAGAAGAAATGTCTACCAAGTCAGTCAA
  AGAGAACAAGGCCCAGGCAGAAGAGGACCTGCAGGAGCCAAAGGGAGACCTGGCAGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200267 representing NM_001033653
  Red=Cloning site Green=Tags(s)
  
  MLGTITITVGQRESEDVREKDRVEEMAANSTAVEDITKDGQEETSEIIEQIPASESNVEEMVQPAESQAN
  DVGFKKVFKFVGFKFTVKKDKNEKSDTVQLLTVKKDEGEGAEASVGAGDHQEPSVETAVGESASKESELK
  QSTEKQEGTLKQEQSSTEIPLQAESDQAAEEEAKDEGEEKQEKEPTKSPESPSSPVSSETTSSFKKFFTH
  GWAGWRKKTSFKKSKEDDLETAEKRKEQEAEKVDEEEKEKTEPASEEQEPAEDTDQARLSADYEKVELPL
  EDQVGDLEASSEEKCAPLATEVFDEKMEAHQEVVAEVHVSTVEKTEEEQGGGGEAEGGVVVEGTGESLPP
  EKLAEPQEVPQEAEPAEELMKSREMCVSGGDHTQLTDLSPEEKTLPKHPEGIVSEVEMLSSQERIKVQGS
  PLKKLFSSSGLKKLSGKKQKGKRGGGGDEEPGEYQHIHTESPESADEQKGESSASSPEEPEETTCLEKGP
  LEAPQDGEAEEGTTSDGEKKREGITPWASFKKMVTPKKRVRRPSESDKEEELEKVKSATLSSTDSTVSEM
  QDEVKTVGEEQKPEEPKRRVDTSVSWEALICVGSSKKRARKASSSDDEGGPRTLGGDSHRAEEASKDKEA
  GTDAVPASTQEQDQAQGSSSPEPAGSPSEGEGVSTWESFKRLVTPRKKSKSKLEEKAEDSSVEQLSTEIE
  PSREESWVSIKKFIPGRRKKRADGKQEQATVEDSGPVEINEDDPDVPAVVPLSEYDAVEREKMEAQGNAE
  LPQLLGAVYVSEELSKTLVHTVSVAVIDGTRAVTSVEERSPSWISASVTEPLEHTAGEAMPPVEEVTEKD
  IIAEETPVLTQTLPEGKDAHDDMVTSEVDFTSEAVTATETSEALRTEEVTEASGAEETTDMVSAVSQLTD
  SPDTTEEATPVQEVEGGVLDTEEEERQTQAILQAVADKVKEESQVPATQTVQRTGSKALEKVEEVEEDSE
  VLASEKEKDVMPKGPVQEAGAEHLAQGSETGQATPESLEVPEVTADVDHVATCQVIKLQQLMEQAVAPES
  SETLTDSETNGSTPLADSDTADGTQQDETIDSQDSKATAAVRQSQVTEEEAATAQKEEPSTLPNNVPAQE
  EHGEEPGRDVLEPTQQELAAAAVPVLAKTEVGQEGEVDWLDGEKVKEEQEVFVHSGPNSQKAADVTYDSE
  VMGVAGCQEKESTEVQSLSLEEGEMETDVEKEKRETKPEQVSEEGEQETAAPEHEGTYGKPVLTLDMPSS
  ERGKALGSLGGSPSLPDQDKAGCIEVQVQSLDTTVTQTAEAVEKVIETVVISETGESPECVGAHLLPAEK
  SSATGGHWTLQHAEDTVPLGPESQAESIPIIVTPAPESTLHPDLQGEISASQRERSEEEDKPDAGPDADG
  KESTAIEKVLKAEPEILELESKSNKIVLNVIQTAVDQFARTETAPETHAYDSQTQVPAMQADSQGAQQML
  DKNESCQDETPSAAAQRGLASPDRSGGMGSASEMLAALAVESAGVKVSIEKLPPQPKDQKEHAADGPQLQ
  SLAQAEASASGNLTKESPDTNGPKLTEEGDAPKVEVQEEEMSTKSVKENKAQAEEDLQEPKGDLAES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033653
• ORF Size: 4821 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033653.1, NP_001028825.1
• RefSeq Size: 6026 bp
• RefSeq ORF: 4824 bp
• Locus ID: 83425
• UniProt ID: Q5QD51
• Cytogenetics: 1q11
• MW: 173.1 kDa
• Gene Summary: Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function]