Akap12 (NM_057103) Rat Tagged ORF Clone

CAT#: RR200266

Akap12 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 12 (Akap12), transcript variant 1, (10 ug)

Product Images

Circular map for RR200266

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akap12
• Synonyms: AKAP12A; AKAP12B; AKAP12G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR200266 representing NM_057103
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCAGGCAGTTCCACCGAGCAGCGGAGCCCCGAGCAGCCGGCGGGGAGCGACACGCCGAGCGAGC
  TGGTGCTCAGTGGCCATGGGCCCGCAGCTGAAGCCTCGGGAGCAGCTGGAGACCCCGCCGACGCGGACCC
  CGCCACCAAGCTCCCACAGAAGAATGGCCAGCTGTCTACTGTCAACGGCGTAGCTGAACAAGGAGATGTC
  CATGTCCAAGAGGAAAACCAGGAGGGGCAGGAGGAAGAAGTCGTTGATGAGGATGTTGGACAGCGAGAGT
  CAGAAGATGTGAGAGAAAAAGACCGAGTTGAAGAAATGGCGGCCAACTCCACAGCTGTTGAAGATATCAC
  AAAGGATGGGCAGGAGGAGACATCAGAAATAATTGAACAGATCCCTGCTTCAGAAAGCAATGTGGAAGAA
  ATGGTACAGCCTGCTGAGTCCCAGGCTAATGATGTTGGCTTCAAGAAAGTATTTAAATTTGTTGGTTTTA
  AATTCACGGTGAAGAAGGATAAAAATGAAAAGTCAGATACTGTCCAACTACTCACTGTCAAGAAGGATGA
  AGGCGAAGGGGCAGAAGCCTCTGTCGGAGCTGGAGACCACCAGGAGCCCAGTGTGGAGACTGCCGTCGGA
  GAGTCAGCATCCAAAGAAAGTGAGCTGAAGCAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGAAC
  AGAGCAGCACAGAAATCCCCCTTCAAGCCGAATCTGATCAAGCGGCTGAGGAAGAAGCCAAAGATGAAGG
  AGAAGAAAAACAAGAGAAAGAGCCCACCAAGTCCCCAGAATCCCCGAGCAGCCCAGTCAGCAGTGAGACA
  ACATCTTCCTTCAAGAAGTTCTTCACTCACGGTTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAAT
  CAAAAGAGGATGATCTGGAAACTGCCGAGAAGAGAAAGGAGCAAGAGGCAGAAAAAGTAGACGAGGAAGA
  AAAGGAAAAGACAGAGCCAGCCTCGGAGGAGCAGGAGCCGGCAGAAGACACAGACCAGGCCAGGTTGTCA
  GCAGACTACGAGAAGGTGGAGCTGCCTTTGGAAGACCAGGTTGGTGACCTGGAGGCATCGTCAGAGGAGA
  AGTGTGCTCCTTTGGCAACGGAAGTGTTTGATGAGAAGATGGAAGCCCACCAAGAAGTTGTTGCAGAGGT
  CCACGTGAGCACCGTGGAGAAGACAGAGGAGGAGCAGGGAGGAGGAGGAGAGGCTGAAGGGGGCGTGGTG
  GTAGAAGGAACAGGAGAATCCTTGCCCCCTGAGAAACTGGCTGAGCCCCAGGAGGTCCCCCAGGAAGCTG
  AGCCTGCTGAGGAGCTGATGAAGAGCAGAGAGATGTGTGTCTCTGGAGGAGACCACACTCAACTGACAGA
  CCTAAGTCCTGAAGAGAAGACGCTGCCCAAACACCCAGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCC
  TCTCAGGAAAGAATCAAGGTACAGGGAAGTCCCTTGAAGAAACTCTTCAGTAGCTCAGGCTTAAAGAAGC
  TGTCTGGGAAGAAGCAGAAGGGGAAACGAGGAGGTGGGGGAGACGAAGAGCCTGGAGAATACCAACACAT
  TCACACCGAATCCCCAGAGAGTGCTGATGAGCAGAAGGGAGAGAGCTCTGCGTCGTCCCCCGAGGAGCCT
  GAGGAGACCACGTGTCTGGAGAAAGGGCCGCTGGAAGCACCCCAGGATGGGGAAGCTGAGGAAGGAACTA
  CTTCCGATGGAGAGAAGAAGAGGGAAGGGATCACTCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAA
  GAAACGGGTCCGAAGACCTTCTGAGAGTGACAAGGAGGAAGAGCTGGAGAAGGTCAAGAGCGCCACCTTG
  TCCTCCACTGATAGCACAGTGTCAGAAATGCAAGATGAAGTCAAAACTGTTGGTGAGGAACAAAAGCCAG
  AGGAACCAAAGCGTAGGGTGGATACTTCAGTGTCTTGGGAAGCACTGATTTGTGTCGGATCATCCAAGAA
  GAGAGCAAGGAAGGCATCCTCTTCAGATGATGAAGGAGGGCCAAGGACACTGGGAGGGGACAGTCACAGA
  GCAGAGGAGGCCAGCAAAGACAAAGAAGCCGGAACAGACGCTGTTCCTGCCAGCACCCAGGAGCAGGACC
  AAGCGCAAGGAAGTTCCTCACCCGAGCCAGCGGGAAGCCCTTCCGAAGGGGAAGGTGTCTCCACTTGGGA
  GTCATTTAAAAGATTAGTCACTCCAAGAAAAAAATCCAAGTCAAAACTGGAAGAGAAAGCCGAAGACTCT
  AGTGTAGAGCAGTTGTCCACTGAGATCGAACCGAGTAGAGAAGAATCTTGGGTTTCCATTAAGAAATTCA
  TCCCCGGACGGCGGAAGAAAAGGGCAGATGGGAAGCAAGAACAAGCCACTGTGGAAGACTCAGGGCCAGT
  GGAGATAAATGAGGACGACCCTGATGTCCCAGCCGTCGTGCCTCTGTCTGAGTATGATGCAGTGGAGAGG
  GAGAAGATGGAAGCCCAGGGGAATGCGGAGCTGCCCCAGCTGCTGGGGGCTGTGTACGTGTCCGAGGAGC
  TCAGTAAGACTCTGGTCCACACTGTGAGTGTCGCAGTCATTGATGGGACCAGGGCAGTCACCAGTGTCGA
  AGAGCGGTCTCCTTCGTGGATATCCGCTTCCGTAACAGAACCTCTTGAACACACAGCGGGAGAAGCCATG
  CCACCTGTTGAAGAGGTCACTGAAAAAGACATCATTGCAGAAGAAACTCCTGTGCTCACCCAGACGTTAC
  CAGAGGGTAAAGATGCCCATGACGACATGGTCACCAGTGAAGTGGATTTCACCTCAGAAGCTGTGACAGC
  CACAGAGACCTCAGAGGCTCTCCGTACTGAAGAAGTTACCGAAGCATCGGGGGCCGAAGAGACCACAGAC
  ATGGTGTCCGCAGTTTCCCAGCTGACTGACTCCCCAGACACCACAGAGGAAGCCACCCCAGTTCAGGAGG
  TAGAGGGTGGTGTGCTAGATACAGAAGAAGAGGAGCGCCAGACGCAGGCCATCCTCCAAGCCGTTGCAGA
  CAAGGTGAAAGAGGAGTCCCAGGTGCCTGCAACCCAGACTGTGCAGAGAACGGGGTCAAAAGCACTGGAG
  AAGGTTGAGGAGGTAGAGGAGGACTCCGAAGTGCTGGCTTCGGAGAAAGAGAAGGACGTTATGCCGAAAG
  GACCCGTGCAGGAAGCTGGAGCTGAGCATCTTGCACAGGGCTCTGAGACTGGACAGGCTACTCCAGAGAG
  CCTTGAAGTTCCTGAAGTCACAGCAGATGTAGACCATGTCGCCACGTGCCAGGTTATCAAGCTCCAGCAG
  CTGATGGAACAGGCCGTGGCCCCTGAGTCATCCGAAACCTTGACAGACAGTGAGACAAATGGAAGCACTC
  CCTTAGCAGATTCAGACACTGCAGATGGGACACAGCAAGATGAAACCATTGACAGCCAGGACAGTAAAGC
  CACTGCAGCTGTCAGGCAGTCACAGGTCACAGAAGAAGAGGCGGCTACTGCTCAGAAAGAGGAGCCTTCG
  ACACTACCTAATAATGTTCCAGCCCAGGAAGAACATGGGGAAGAACCAGGAAGAGATGTTCTTGAACCTA
  CACAGCAAGAGCTTGCTGCTGCAGCCGTGCCCGTTCTGGCAAAGACTGAGGTGGGTCAAGAGGGTGAGGT
  TGACTGGTTGGATGGAGAAAAAGTCAAAGAAGAACAGGAGGTGTTTGTACACTCTGGACCCAACAGTCAA
  AAGGCTGCTGATGTGACATATGACAGTGAAGTGATGGGAGTGGCCGGGTGTCAGGAAAAGGAGAGTACTG
  AAGTGCAGAGTCTTAGCCTGGAGGAGGGAGAGATGGAAACTGACGTTGAAAAGGAGAAAAGGGAGACAAA
  GCCAGAGCAAGTGAGTGAAGAAGGTGAGCAGGAAACAGCCGCTCCTGAGCATGAAGGAACCTACGGGAAG
  CCAGTCCTGACACTTGACATGCCCAGCTCAGAGAGGGGGAAGGCACTGGGAAGCCTTGGAGGAAGCCCTT
  CTCTCCCAGACCAAGACAAAGCAGGTTGCATAGAGGTTCAAGTTCAAAGCCTGGACACAACAGTCACTCA
  AACAGCAGAAGCTGTGGAAAAGGTCATAGAAACGGTTGTGATTTCAGAGACAGGTGAAAGTCCAGAGTGT
  GTAGGTGCACACTTATTACCAGCTGAGAAGTCCTCTGCAACGGGTGGCCACTGGACTCTTCAGCATGCAG
  AGGACACGGTACCCCTGGGGCCTGAGTCTCAGGCAGAATCCATCCCAATCATAGTAACTCCTGCTCCTGA
  AAGCACCCTACATCCTGACCTACAAGGAGAAATAAGCGCATCCCAGAGAGAGCGATCAGAGGAAGAGGAC
  AAGCCAGATGCTGGTCCTGATGCTGACGGCAAGGAGAGTACAGCAATCGAAAAAGTCCTCAAGGCTGAAC
  CTGAGATCCTGGAACTTGAGAGTAAGAGCAACAAGATTGTGCTGAACGTCATTCAGACAGCCGTTGACCA
  GTTCGCACGTACAGAAACAGCCCCCGAAACTCATGCTTATGATTCACAGACCCAGGTTCCTGCAATGCAG
  GCTGACAGCCAGGGAGCCCAACAGATGCTGGACAAAAATGAAAGTTGCCAAGATGAAACACCCAGTGCCG
  CAGCCCAGAGAGGACTTGCAAGTCCTGACCGTTCTGGAGGCATGGGCTCAGCCTCGGAAATGCTTGCCGC
  GCTTGCAGTTGAAAGCGCCGGTGTCAAAGTAAGCATTGAGAAGCTGCCTCCTCAACCCAAAGATCAAAAG
  GAGCATGCTGCTGATGGCCCTCAGCTCCAAAGCTTAGCCCAGGCAGAGGCCAGTGCCTCTGGAAACCTAA
  CCAAAGAATCCCCAGACACCAACGGACCAAAGCTAACCGAGGAGGGCGATGCCCCAAAAGTTGAGGTCCA
  GGAAGAAGAAATGTCTACCAAGTCAGTCAAAGAGAACAAGGCCCAGGCAGAAGAGGACCTGCAGGAGCCA
  AAGGGAGACCTGGCAGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR200266 representing NM_057103
  Red=Cloning site Green=Tags(s)
  
  MGAGSSTEQRSPEQPAGSDTPSELVLSGHGPAAEASGAAGDPADADPATKLPQKNGQLSTVNGVAEQGDV
  HVQEENQEGQEEEVVDEDVGQRESEDVREKDRVEEMAANSTAVEDITKDGQEETSEIIEQIPASESNVEE
  MVQPAESQANDVGFKKVFKFVGFKFTVKKDKNEKSDTVQLLTVKKDEGEGAEASVGAGDHQEPSVETAVG
  ESASKESELKQSTEKQEGTLKQEQSSTEIPLQAESDQAAEEEAKDEGEEKQEKEPTKSPESPSSPVSSET
  TSSFKKFFTHGWAGWRKKTSFKKSKEDDLETAEKRKEQEAEKVDEEEKEKTEPASEEQEPAEDTDQARLS
  ADYEKVELPLEDQVGDLEASSEEKCAPLATEVFDEKMEAHQEVVAEVHVSTVEKTEEEQGGGGEAEGGVV
  VEGTGESLPPEKLAEPQEVPQEAEPAEELMKSREMCVSGGDHTQLTDLSPEEKTLPKHPEGIVSEVEMLS
  SQERIKVQGSPLKKLFSSSGLKKLSGKKQKGKRGGGGDEEPGEYQHIHTESPESADEQKGESSASSPEEP
  EETTCLEKGPLEAPQDGEAEEGTTSDGEKKREGITPWASFKKMVTPKKRVRRPSESDKEEELEKVKSATL
  SSTDSTVSEMQDEVKTVGEEQKPEEPKRRVDTSVSWEALICVGSSKKRARKASSSDDEGGPRTLGGDSHR
  AEEASKDKEAGTDAVPASTQEQDQAQGSSSPEPAGSPSEGEGVSTWESFKRLVTPRKKSKSKLEEKAEDS
  SVEQLSTEIEPSREESWVSIKKFIPGRRKKRADGKQEQATVEDSGPVEINEDDPDVPAVVPLSEYDAVER
  EKMEAQGNAELPQLLGAVYVSEELSKTLVHTVSVAVIDGTRAVTSVEERSPSWISASVTEPLEHTAGEAM
  PPVEEVTEKDIIAEETPVLTQTLPEGKDAHDDMVTSEVDFTSEAVTATETSEALRTEEVTEASGAEETTD
  MVSAVSQLTDSPDTTEEATPVQEVEGGVLDTEEEERQTQAILQAVADKVKEESQVPATQTVQRTGSKALE
  KVEEVEEDSEVLASEKEKDVMPKGPVQEAGAEHLAQGSETGQATPESLEVPEVTADVDHVATCQVIKLQQ
  LMEQAVAPESSETLTDSETNGSTPLADSDTADGTQQDETIDSQDSKATAAVRQSQVTEEEAATAQKEEPS
  TLPNNVPAQEEHGEEPGRDVLEPTQQELAAAAVPVLAKTEVGQEGEVDWLDGEKVKEEQEVFVHSGPNSQ
  KAADVTYDSEVMGVAGCQEKESTEVQSLSLEEGEMETDVEKEKRETKPEQVSEEGEQETAAPEHEGTYGK
  PVLTLDMPSSERGKALGSLGGSPSLPDQDKAGCIEVQVQSLDTTVTQTAEAVEKVIETVVISETGESPEC
  VGAHLLPAEKSSATGGHWTLQHAEDTVPLGPESQAESIPIIVTPAPESTLHPDLQGEISASQRERSEEED
  KPDAGPDADGKESTAIEKVLKAEPEILELESKSNKIVLNVIQTAVDQFARTETAPETHAYDSQTQVPAMQ
  ADSQGAQQMLDKNESCQDETPSAAAQRGLASPDRSGGMGSASEMLAALAVESAGVKVSIEKLPPQPKDQK
  EHAADGPQLQSLAQAEASASGNLTKESPDTNGPKLTEEGDAPKVEVQEEEMSTKSVKENKAQAEEDLQEP
  KGDLAES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057103
• ORF Size: 5061 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057103.2, NP_476444.2
• RefSeq Size: 6255 bp
• RefSeq ORF: 5064 bp
• Locus ID: 83425
• UniProt ID: Q5QD51
• Cytogenetics: 1q11
• MW: 181.1 kDa
• Gene Summary: Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function]