Tpm3 (NM_173111) Rat Tagged ORF Clone
CAT#: RR200218
- TrueORF®
Tpm3 (Myc-DDK-tagged ORF) - Rat tropomyosin 3, gamma (Tpm3), transcript variant 2, (10 ug)
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_173111" in other vectors (3)
USD 198.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tpm3 |
Synonyms | TM30nm; Tpm5 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>NM_173111 ORF sequence, RR200218 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGGGAGCACCACCATCGAGGCGGTAAAGCGCAAGATCCAGGTTCTGCAGCAGCAGGCTGATGAT GCGGAGGAGAGGGCCGAGCGCCTCCAGCGGGAAGTGGAGGGAGAAAGGCGGGCCCGGGAGCAGGCTGAA GCTGAGGTGGCCTCCTTGAACCGCAGGATCCAGCTGGTTGAAGAGGAGCTGGACCGCGCCCAGGAGCGC CTTGCCACTGCTTTGCAGAAGCTGGAGGAAGCGGAGAAGGCTGCTGATGAGAGTGAGAGAGGTATGAAG GTGATTGAAAACCGGGCTCTAAAAGATGAAGAAAAGATGGAGCTCCAGGAAATCCAGCTGAAGGAAGCA AAGCACATTGCAGAAGAGGCAGACAGGAAGTATGAAGAGGTGGCTCGTAAGTTGGTGATTATTGAAGGA GACTTGGAACGCACGGAGGAACGCGCCGAGCTGGCAGAGTCCCGTTGCCGAGAGATGGATGAGCAGATC AGACTGATGGACCAGAACCTGAAGTGTCTGAGTGCTGCTGAAGAAAAGTACTCTCAAAAAGAAGACAAG TATGAAGAAGAAATAAAGATTCTTACTGATAAACTCAAGGAGGCGGAGACCCGGGCTGAGTTTGCTGAA AGATCGGTAGCCAAACTGGAAAAGACCATTGATGACTTGGAAGATAAGCTGAAGTGCACCAAAGAGGAG CATCTCTGTACACAAAGGATGCTGGACCAGACCCTGCTGGACCTGAACGAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RR200218
Blue=ORF Red=Cloning site Green=Tag(s) MAGSTTIEAVKRKIQVLQQQADDAEERAERLQREVEGERRAREQAEAEVASLNRRIQLVEEELDRAQER LATALQKLEEAEKAADESERGMKVIENRALKDEEKMELQEIQLKEAKHIAEEADRKYEEVARKLVIIEG DLERTEERAELAESRCREMDEQIRLMDQNLKCLSAAEEKYSQKEDKYEEEIKILTDKLKEAETRAEFAE RSVAKLEKTIDDLEDKLKCTKEEHLCTQRMLDQTLLDLNEM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_173111 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_173111.2 |
RefSeq Size | 1770 bp |
RefSeq ORF | 747 bp |
Locus ID | 117557 |
UniProt ID | Q63610 |
Cytogenetics | 2q34 |
MW | 29 kDa |
Gene Summary | rod-like protein associated with actin filament in nonmuscle cells; may mediate the stability of actin filaments; may play a role in the regulation of cell transformation, cytokinesis, motility, and morphogenesis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200218 | Tpm3 (untagged ORF) - Rat tropomyosin 3, gamma (Tpm3), transcript variant 2, (10 ug) |
USD 330.00 |
|
RR200218L3 | Lenti ORF clone of Tpm3 (Myc-DDK-tagged ORF) - Rat tropomyosin 3, gamma (Tpm3), transcript variant 2, (10 ug) |
USD 630.00 |
|
RR200218L4 | Lenti ORF clone of Tpm3 (mGFP-tagged ORF) - Rat tropomyosin 3, gamma (Tpm3), transcript variant 2, (10 ug) |
USD 630.00 |
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