Ttc5 (NM_001013131) Rat Untagged Clone

CAT#: RN212276

Ttc5 (untagged ORF) - Rat tetratricopeptide repeat domain 5 (Ttc5), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Ttc5
• Synonyms: MGC108914
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN212276 representing NM_001013131
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTGATGAAGAGGAGGAAGCGAAGCAGGTCTTGCAGAAATTGCAGGAACTGGTGGATCAGCTCT
  ACTGTTTTCGAGACAGTTACTTTGAGACACACAGTGTGGAAGATGCAGGACAGAAGCAGCAGGATGTTCA
  GGAAGAGATGGAGAAGACCCTGCGGCAGATGGAGGAAGTACTTGGTTCTGTCCAGGTTGAGGCACAGGCT
  CTAATGCTCAAGGGGAAGGCACTGAATGTGACTCCTGATTACAGCCCGGAGGCCGAGGTGCTGCTCTCAA
  AGGCCGTGAAGCTGGAGCCTGAACTGGTGGAAGCCTGGAACCAGTTGGGTGAGGTGTACTGGAAGAAAGG
  AGATGTTGCGGCTGCCCACACCTGCTTCTCAGGAGCCCTCACCCACTGCAAGAACAAGGTCTCTCTGCAG
  AACTTGTCCATGGTGCTCCGCCAGTTGCAGACCGACTCCGGAGATGAACATTCTCGCCACGTCATGGATA
  GTGTCCGACAGGCCAAGTTGGCTGTGCAGATGGACGTCCTTGATGGCCGCTCCTGGTATATCCTGGGGAA
  TGCATATCTTTCTCTTTATTTCAATACTGGCCAGAACCCTAAGATCTCCCAGCAAGCCCTCAGTGCCTAT
  GCTCAAGCAGAGAAGGTCGACAGGAAAGCATCCAGCAACCCTGACCTTCATCTCAACAGGGCGACGTTAC
  ATAAATATGAAGAGAGTTATGGGGAGGCCCTAGAGGGCTTCTCTCAGGCTGCCACGCTGGACCCTGCATG
  GCCAGAGCCCCAACAACGAGAACAGCAGCTCCTGGAATTCCTCAGTAGGCTGACCAACCTCCTGGCGAGC
  AAGGGGAAGACAAAACCCAAGAAGCTTCAGAGCATGCTGGGAAACTTGCGCCCGGCCCATCTGGGCCCTT
  GTGGCGACGGGCGCTACCAGTCAGCCACCGGGCAGAAGGTGACCCTGCAGCTCAAGCCACTGAGTACGCT
  GCAGCCTGGTGTGAACAGCGGCACCGTGGTCCTGGGAAAGGTGGTGTTCAGCCTGACCACAGAGGAGAAG
  GTCCCCTTCACATTTGGCTTGGTAGATTCAGACGGTCCTTGCTATGCCGTGATGGTGTATAATGTGGTGC
  AGAGCTGGGGAGTTCTCATTGGGGACTCTGTAGCCATTCCTGAGCCCAACCTGAGGCATCACCAAATTCA
  GCACAAGGGAAAGGACTATTCCTTCTCCAGCGTTCGCGTGGAAACGCCTCTTCTGCTGGTGGTGAATGGA
  AAGCCACAGAACTCCAGCAGCCAAGCCTCTGCCACAGTGGCTTCAAGGCCACAGTGTGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013131
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013131.1, NP_001013149.1
• RefSeq Size: 1955 bp
• RefSeq ORF: 1323 bp
• Locus ID: 305837
• UniProt ID: Q5BK48
• Cytogenetics: 15p14
• Gene Summary: Adapter protein involved in p53/TP53 response that acts by regulating and mediating the assembly of multi-protein complexes. Required to facilitate the interaction between JMY and p300/EP300 and increase p53/TP53-dependent transcription and apoptosis. Prevents p53/TP53 degradation by MDM2 (By similarity).[UniProtKB/Swiss-Prot Function]