Ttc5 (NM_001013131) Rat Tagged ORF Clone

CAT#: RR212276

Ttc5 (Myc-DDK-tagged ORF) - Rat tetratricopeptide repeat domain 5 (Ttc5), (10 ug)

Product Images

Circular map for RR212276

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ttc5
• Synonyms: MGC108914
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR212276 representing NM_001013131
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTGATGAAGAGGAGGAAGCGAAGCAGGTCTTGCAGAAATTGCAGGAACTGGTGGATCAGCTCT
  ACTGTTTTCGAGACAGTTACTTTGAGACACACAGTGTGGAAGATGCAGGACAGAAGCAGCAGGATGTTCA
  GGAAGAGATGGAGAAGACCCTGCGGCAGATGGAGGAAGTACTTGGTTCTGTCCAGGTTGAGGCACAGGCT
  CTAATGCTCAAGGGGAAGGCACTGAATGTGACTCCTGATTACAGCCCGGAGGCCGAGGTGCTGCTCTCAA
  AGGCCGTGAAGCTGGAGCCTGAACTGGTGGAAGCCTGGAACCAGTTGGGTGAGGTGTACTGGAAGAAAGG
  AGATGTTGCGGCTGCCCACACCTGCTTCTCAGGAGCCCTCACCCACTGCAAGAACAAGGTCTCTCTGCAG
  AACTTGTCCATGGTGCTCCGCCAGTTGCAGACCGACTCCGGAGATGAACATTCTCGCCACGTCATGGATA
  GTGTCCGACAGGCCAAGTTGGCTGTGCAGATGGACGTCCTTGATGGCCGCTCCTGGTATATCCTGGGGAA
  TGCATATCTTTCTCTTTATTTCAATACTGGCCAGAACCCTAAGATCTCCCAGCAAGCCCTCAGTGCCTAT
  GCTCAAGCAGAGAAGGTCGACAGGAAAGCATCCAGCAACCCTGACCTTCATCTCAACAGGGCGACGTTAC
  ATAAATATGAAGAGAGTTATGGGGAGGCCCTAGAGGGCTTCTCTCAGGCTGCCACGCTGGACCCTGCATG
  GCCAGAGCCCCAACAACGAGAACAGCAGCTCCTGGAATTCCTCAGTAGGCTGACCAACCTCCTGGCGAGC
  AAGGGGAAGACAAAACCCAAGAAGCTTCAGAGCATGCTGGGAAACTTGCGCCCGGCCCATCTGGGCCCTT
  GTGGCGACGGGCGCTACCAGTCAGCCACCGGGCAGAAGGTGACCCTGCAGCTCAAGCCACTGAGTACGCT
  GCAGCCTGGTGTGAACAGCGGCACCGTGGTCCTGGGAAAGGTGGTGTTCAGCCTGACCACAGAGGAGAAG
  GTCCCCTTCACATTTGGCTTGGTAGATTCAGACGGTCCTTGCTATGCCGTGATGGTGTATAATGTGGTGC
  AGAGCTGGGGAGTTCTCATTGGGGACTCTGTAGCCATTCCTGAGCCCAACCTGAGGCATCACCAAATTCA
  GCACAAGGGAAAGGACTATTCCTTCTCCAGCGTTCGCGTGGAAACGCCTCTTCTGCTGGTGGTGAATGGA
  AAGCCACAGAACTCCAGCAGCCAAGCCTCTGCCACAGTGGCTTCAAGGCCACAGTGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR212276 representing NM_001013131
  Red=Cloning site Green=Tags(s)
  
  MMADEEEEAKQVLQKLQELVDQLYCFRDSYFETHSVEDAGQKQQDVQEEMEKTLRQMEEVLGSVQVEAQA
  LMLKGKALNVTPDYSPEAEVLLSKAVKLEPELVEAWNQLGEVYWKKGDVAAAHTCFSGALTHCKNKVSLQ
  NLSMVLRQLQTDSGDEHSRHVMDSVRQAKLAVQMDVLDGRSWYILGNAYLSLYFNTGQNPKISQQALSAY
  AQAEKVDRKASSNPDLHLNRATLHKYEESYGEALEGFSQAATLDPAWPEPQQREQQLLEFLSRLTNLLAS
  KGKTKPKKLQSMLGNLRPAHLGPCGDGRYQSATGQKVTLQLKPLSTLQPGVNSGTVVLGKVVFSLTTEEK
  VPFTFGLVDSDGPCYAVMVYNVVQSWGVLIGDSVAIPEPNLRHHQIQHKGKDYSFSSVRVETPLLLVVNG
  KPQNSSSQASATVASRPQCE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013131
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013131.1, NP_001013149.1
• RefSeq Size: 1955 bp
• RefSeq ORF: 1323 bp
• Locus ID: 305837
• UniProt ID: Q5BK48
• Cytogenetics: 15p14
• MW: 48.8 kDa
• Gene Summary: Adapter protein involved in p53/TP53 response that acts by regulating and mediating the assembly of multi-protein complexes. Required to facilitate the interaction between JMY and p300/EP300 and increase p53/TP53-dependent transcription and apoptosis. Prevents p53/TP53 degradation by MDM2 (By similarity).[UniProtKB/Swiss-Prot Function]