Coq6 (NM_001011983) Rat Untagged Clone

CAT#: RN211896

Coq6 (untagged ORF) - Rat coenzyme Q6 homolog (yeast) (Coq6), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Coq6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN211896 representing NM_001011983
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCGCATTGGCCCCATGGCAGGTTTGCTCTGTGTTCGCTGGTGGAGTACCGCGCAACTTGCAG
  CCAGAGGAGGCCCACTAGTTGCCTGTCGGCGATGGACGAGCTCCTCGACAGACTCCGTGTACGATGTGGT
  GGTGTCAGGAGGAGGCATGGTGGGCTCTGCCATGGCTTGTGCCTTGGGACATGATATTCACTTTCATGAC
  AAGAAAATCCTATTGCTGGAAGCGGGTCCGAAGAAAACCCTGGAGAAACTGTCAGAAACTTACAGCAACA
  GAGTCAGCTCCATATCCCTTGGCTCCACAACCCTTCTCAGCAGTTTTGGTGCATGGGACCACATCTGCAA
  TATGCGTTGCAAAGCCTTCCGGCGGATGCAGGTTTGGGACTCCTGCTCAGAGGCCTTGATAATGTTCGAC
  AAGGATAACTTGGATGACATGGGCTATATAGTGGAAAATGACGTCATCATGCACGCCATCACCAAGCAGC
  TGGAGGCGGTGGCAGATCGAGTGAAGGTTCTCTACGAGAGCAAAGCTGTTGGCTATGCCTGGCCTGGTCC
  ATTTTCCTTGGCAGACTCCAGCCCTTGGGTCCATATTACCCTAGGTGATGGCAGCACCCTCCAGACCAAG
  CTGTTGATTGGTGCTGATGGCCACAACTCAGGAGTGAGGCAAGCTGCTGGGATCCAGAATGTTGGCTGGA
  ACTACGACCAGTCTGCTGTGGTGGCCACTCTCCACTTATCAGAGGCCACAGAAAACAATGTAGCCTGGCA
  GAGATTTCTTCCCTCTGGGCCTATTGCTCTGCTCCCGCTCTCAGATACCCTGAGTTCCTTGGTGTGGTCC
  ACGTCACATGCCCATGCAGCCGAGCTGGTCAGCATGCATGAGGAAGAGTTCGTGGATGCCATCAACTCGG
  CCTTTTGGAGTGATGTTCACCACACAGACTTCGTCGACTCAGCTAGTGCCATGGTGCACCATGCTGTCGC
  CCTTCTGAAGCCCACTAAGGTCTCCGCCCGCCAGCTGCCCCCAAGCGTAGCCAAGGTGGATGCCAAGAGC
  AGAGCCCTCTTTCCTCTGGGGCTGGGACATGCTGCTGAGTACGTCCGGCCTCGGGTGGCCCTCATCGGTG
  ATGCAGCCCACAGAGTCCATCCACTTGCAGGACAAGGTGTCAACATGGGCTTCGGGGATATTTCCAGCTT
  GATTCATTATCTCAGTACAGCAGCCTTCAATGGCAAGGACTTAGGCTCCATGAGCCACCTCACAGGCTAT
  GAAACAGACCGACAGCGTCATAATACTGCTCTTCTGGCTGCTACTGACCTGCTGAAAAGGCTCTACTCTA
  CCAGTACCACTCCGCTAGTGTTGCTCAGGACCTGGGGCCTGCAGGCCACTAATGCAGTGTCTCCTCTCAA
  AGAACAGATTATGGCCTTTGCGAGTAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011983
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011983.1, NP_001011983.1
• RefSeq Size: 1762 bp
• RefSeq ORF: 1431 bp
• Locus ID: 299195
• UniProt ID: Q68FU7
• Cytogenetics: 6q31
• Gene Summary: FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3-polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6.[UniProtKB/Swiss-Prot Function]