Coq6 (NM_001011983) Rat Tagged ORF Clone

CAT#: RR211896

Coq6 (Myc-DDK-tagged ORF) - Rat coenzyme Q6 homolog (yeast) (Coq6), (10 ug)

Product Images

Circular map for RR211896

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Coq6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR211896 representing NM_001011983
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCGCATTGGCCCCATGGCAGGTTTGCTCTGTGTTCGCTGGTGGAGTACCGCGCAACTTGCAG
  CCAGAGGAGGCCCACTAGTTGCCTGTCGGCGATGGACGAGCTCCTCGACAGACTCCGTGTACGATGTGGT
  GGTGTCAGGAGGAGGCATGGTGGGCTCTGCCATGGCTTGTGCCTTGGGACATGATATTCACTTTCATGAC
  AAGAAAATCCTATTGCTGGAAGCGGGTCCGAAGAAAACCCTGGAGAAACTGTCAGAAACTTACAGCAACA
  GAGTCAGCTCCATATCCCTTGGCTCCACAACCCTTCTCAGCAGTTTTGGTGCATGGGACCACATCTGCAA
  TATGCGTTGCAAAGCCTTCCGGCGGATGCAGGTTTGGGACTCCTGCTCAGAGGCCTTGATAATGTTCGAC
  AAGGATAACTTGGATGACATGGGCTATATAGTGGAAAATGACGTCATCATGCACGCCATCACCAAGCAGC
  TGGAGGCGGTGGCAGATCGAGTGAAGGTTCTCTACGAGAGCAAAGCTGTTGGCTATGCCTGGCCTGGTCC
  ATTTTCCTTGGCAGACTCCAGCCCTTGGGTCCATATTACCCTAGGTGATGGCAGCACCCTCCAGACCAAG
  CTGTTGATTGGTGCTGATGGCCACAACTCAGGAGTGAGGCAAGCTGCTGGGATCCAGAATGTTGGCTGGA
  ACTACGACCAGTCTGCTGTGGTGGCCACTCTCCACTTATCAGAGGCCACAGAAAACAATGTAGCCTGGCA
  GAGATTTCTTCCCTCTGGGCCTATTGCTCTGCTCCCGCTCTCAGATACCCTGAGTTCCTTGGTGTGGTCC
  ACGTCACATGCCCATGCAGCCGAGCTGGTCAGCATGCATGAGGAAGAGTTCGTGGATGCCATCAACTCGG
  CCTTTTGGAGTGATGTTCACCACACAGACTTCGTCGACTCAGCTAGTGCCATGGTGCACCATGCTGTCGC
  CCTTCTGAAGCCCACTAAGGTCTCCGCCCGCCAGCTGCCCCCAAGCGTAGCCAAGGTGGATGCCAAGAGC
  AGAGCCCTCTTTCCTCTGGGGCTGGGACATGCTGCTGAGTACGTCCGGCCTCGGGTGGCCCTCATCGGTG
  ATGCAGCCCACAGAGTCCATCCACTTGCAGGACAAGGTGTCAACATGGGCTTCGGGGATATTTCCAGCTT
  GATTCATTATCTCAGTACAGCAGCCTTCAATGGCAAGGACTTAGGCTCCATGAGCCACCTCACAGGCTAT
  GAAACAGACCGACAGCGTCATAATACTGCTCTTCTGGCTGCTACTGACCTGCTGAAAAGGCTCTACTCTA
  CCAGTACCACTCCGCTAGTGTTGCTCAGGACCTGGGGCCTGCAGGCCACTAATGCAGTGTCTCCTCTCAA
  AGAACAGATTATGGCCTTTGCGAGTAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR211896 representing NM_001011983
  Red=Cloning site Green=Tags(s)
  
  MAARIGPMAGLLCVRWWSTAQLAARGGPLVACRRWTSSSTDSVYDVVVSGGGMVGSAMACALGHDIHFHD
  KKILLLEAGPKKTLEKLSETYSNRVSSISLGSTTLLSSFGAWDHICNMRCKAFRRMQVWDSCSEALIMFD
  KDNLDDMGYIVENDVIMHAITKQLEAVADRVKVLYESKAVGYAWPGPFSLADSSPWVHITLGDGSTLQTK
  LLIGADGHNSGVRQAAGIQNVGWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWS
  TSHAHAAELVSMHEEEFVDAINSAFWSDVHHTDFVDSASAMVHHAVALLKPTKVSARQLPPSVAKVDAKS
  RALFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLIHYLSTAAFNGKDLGSMSHLTGY
  ETDRQRHNTALLAATDLLKRLYSTSTTPLVLLRTWGLQATNAVSPLKEQIMAFASR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011983
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011983.1, NP_001011983.1
• RefSeq Size: 1762 bp
• RefSeq ORF: 1431 bp
• Locus ID: 299195
• UniProt ID: Q68FU7
• Cytogenetics: 6q31
• MW: 51.5 kDa
• Gene Summary: FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3-polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6.[UniProtKB/Swiss-Prot Function]