Tat (NM_012668) Rat Untagged Clone

CAT#: RN206539

Tat (untagged ORF) - Rat tyrosine aminotransferase (Tat), nuclear gene encoding mitochondrial protein, (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Tat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN206539 representing NM_012668
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCTACGTGATTCAGACGGATGTCGACGACAGCTTGTCCTCAGTTCTGGATGTGCATGTCAATA
  TTGGTGGGAGAAACTCGGTACAAGGAAGAAAGAAAGGCAGGAAGGCCAGATGGGACGTGAGACCCTCTGA
  CATGTCCAATAAGACCTTCAATCCCATCCGAGCCATCGTGGACAACATGAAGGTGCAGCCCAATCCGAAC
  AAGACCGTGATTTCTCTGTCAATTGGGGACCCTACTGTGTTTGGGAACCTGCCTACAGACCCTGAAGTTA
  CCCAAGCCATGAAAGATGCCCTGGACTCGGGGAAGTACAATGGCTATGCCCCGTCCATCGGCTACCTATC
  CAGTCGGGAGGAGGTCGCTTCTTACTACCACTGTCATGAGGCTCCTCTGGAAGCTAAGGATGTCATTCTG
  ACAAGCGGCTGCAGTCAGGCCATTGAGCTATGTCTAGCTGTGTTGGCCAATCCTGGACAAAACATCCTCA
  TTCCAAGGCCCGGGTTTTCCCTCTATAGGACTTTGGCTGAGTCTATGGGAATTGAGGTCAAGCTCTACAA
  TCTCCTGCCCGAGAAGTCTTGGGAAATTGACCTAAAACAACTGGAATCTCTGATCGATGAAAAAACAGCG
  TGTCTTGTTGTCAACAACCCATCCAATCCCTGTGGCTCCGTGTTCAGTAAGCGGCACCTTCAGAAGATTT
  TGGCAGTGGCTGAAAGGCAGTGTGTCCCCATCTTAGCTGACGAGATCTATGGTGACATGGTGTTTTCAGA
  TTGCAAATACGAACCACTGGCCAACCTCAGCACCAATGTTCCCATCCTGTCCTGTGGTGGGCTGGCCAAG
  CGCTGGCTGGTTCCTGGCTGGAGGTTGGGCTGGATCCTCATTCATGATCGAAGAGACATTTTTGGCAATG
  AGATTCGAGACGGGCTGGTGAAACTGAGTCAGCGGATCCTGGGACCATGCACCATAGTCCAGGGTGCTCT
  GAAGAGCATCCTTCAGCGAACCCCTCAGGAGTTCTATCACGACACGTTAAGCTTCCTCAAGTCCAATGCG
  GACCTCTGCTATGGGGCACTGGCTGCCATCCCTGGACTCCAGCCGGTCCGCCCTTCTGGAGCCATGTACC
  TTATGGTGGGAATTGAGATGGAGCATTTCCCGGAATTCGAGAACGACGTGGAGTTCACAGAGCGGTTGAT
  TGCGGAGCAGGCTGTCCACTGTCTCCCAGCAACGTGCTTCGAGTACCCAAATTTCTTCCGAGTGGTCATC
  ACAGTCCCCGAGGTGATGATGCTGGAGGCTTGTAGCCGGATCCAGGAGTTCTGTGAACAGCACTACCACT
  GTGCTGAAGGCAGCCAGGAGGAGTGTGACAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012668
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012668.2, NP_036800.1
• RefSeq Size: 2037 bp
• RefSeq ORF: 1365 bp
• Locus ID: 24813
• UniProt ID: P04694
• Cytogenetics: 19q12
• Gene Summary: catalyzes the conversion of L-tyrosine and 2-oxoglutarate to 4-hydroxyphenylpyruvate and L-glutamate; involved in gluconeogenesis [RGD, Feb 2006]