Tat (NM_012668) Rat Tagged ORF Clone

CAT#: RR206539

Tat (Myc-DDK-tagged ORF) - Rat tyrosine aminotransferase (Tat), nuclear gene encoding mitochondrial protein, (10 ug)

Product Images

Circular map for RR206539

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR206539 representing NM_012668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCTACGTGATTCAGACGGATGTCGACGACAGCTTGTCCTCAGTTCTGGATGTGCATGTCAATA
  TTGGTGGGAGAAACTCGGTACAAGGAAGAAAGAAAGGCAGGAAGGCCAGATGGGACGTGAGACCCTCTGA
  CATGTCCAATAAGACCTTCAATCCCATCCGAGCCATCGTGGACAACATGAAGGTGCAGCCCAATCCGAAC
  AAGACCGTGATTTCTCTGTCAATTGGGGACCCTACTGTGTTTGGGAACCTGCCTACAGACCCTGAAGTTA
  CCCAAGCCATGAAAGATGCCCTGGACTCGGGGAAGTACAATGGCTATGCCCCGTCCATCGGCTACCTATC
  CAGTCGGGAGGAGGTCGCTTCTTACTACCACTGTCATGAGGCTCCTCTGGAAGCTAAGGATGTCATTCTG
  ACAAGCGGCTGCAGTCAGGCCATTGAGCTATGTCTAGCTGTGTTGGCCAATCCTGGACAAAACATCCTCA
  TTCCAAGGCCCGGGTTTTCCCTCTATAGGACTTTGGCTGAGTCTATGGGAATTGAGGTCAAGCTCTACAA
  TCTCCTGCCCGAGAAGTCTTGGGAAATTGACCTAAAACAACTGGAATCTCTGATCGATGAAAAAACAGCG
  TGTCTTGTTGTCAACAACCCATCCAATCCCTGTGGCTCCGTGTTCAGTAAGCGGCACCTTCAGAAGATTT
  TGGCAGTGGCTGAAAGGCAGTGTGTCCCCATCTTAGCTGACGAGATCTATGGTGACATGGTGTTTTCAGA
  TTGCAAATACGAACCACTGGCCAACCTCAGCACCAATGTTCCCATCCTGTCCTGTGGTGGGCTGGCCAAG
  CGCTGGCTGGTTCCTGGCTGGAGGTTGGGCTGGATCCTCATTCATGATCGAAGAGACATTTTTGGCAATG
  AGATTCGAGACGGGCTGGTGAAACTGAGTCAGCGGATCCTGGGACCATGCACCATAGTCCAGGGTGCTCT
  GAAGAGCATCCTTCAGCGAACCCCTCAGGAGTTCTATCACGACACGTTAAGCTTCCTCAAGTCCAATGCG
  GACCTCTGCTATGGGGCACTGGCTGCCATCCCTGGACTCCAGCCGGTCCGCCCTTCTGGAGCCATGTACC
  TTATGGTGGGAATTGAGATGGAGCATTTCCCGGAATTCGAGAACGACGTGGAGTTCACAGAGCGGTTGAT
  TGCGGAGCAGGCTGTCCACTGTCTCCCAGCAACGTGCTTCGAGTACCCAAATTTCTTCCGAGTGGTCATC
  ACAGTCCCCGAGGTGATGATGCTGGAGGCTTGTAGCCGGATCCAGGAGTTCTGTGAACAGCACTACCACT
  GTGCTGAAGGCAGCCAGGAGGAGTGTGACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR206539 representing NM_012668
  Red=Cloning site Green=Tags(s)
  
  MDSYVIQTDVDDSLSSVLDVHVNIGGRNSVQGRKKGRKARWDVRPSDMSNKTFNPIRAIVDNMKVQPNPN
  KTVISLSIGDPTVFGNLPTDPEVTQAMKDALDSGKYNGYAPSIGYLSSREEVASYYHCHEAPLEAKDVIL
  TSGCSQAIELCLAVLANPGQNILIPRPGFSLYRTLAESMGIEVKLYNLLPEKSWEIDLKQLESLIDEKTA
  CLVVNNPSNPCGSVFSKRHLQKILAVAERQCVPILADEIYGDMVFSDCKYEPLANLSTNVPILSCGGLAK
  RWLVPGWRLGWILIHDRRDIFGNEIRDGLVKLSQRILGPCTIVQGALKSILQRTPQEFYHDTLSFLKSNA
  DLCYGALAAIPGLQPVRPSGAMYLMVGIEMEHFPEFENDVEFTERLIAEQAVHCLPATCFEYPNFFRVVI
  TVPEVMMLEACSRIQEFCEQHYHCAEGSQEECDK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012668
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012668.2, NP_036800.1
• RefSeq Size: 2037 bp
• RefSeq ORF: 1365 bp
• Locus ID: 24813
• UniProt ID: P04694
• Cytogenetics: 19q12
• MW: 50.6 kDa
• Gene Summary: catalyzes the conversion of L-tyrosine and 2-oxoglutarate to 4-hydroxyphenylpyruvate and L-glutamate; involved in gluconeogenesis [RGD, Feb 2006]