Gba (NM_001127639) Rat Untagged Clone

CAT#: RN205005

Gba (untagged ORF) - Rat glucosidase, beta, acid (Gba), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Gba
• Synonyms: MGC188451
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN205005 representing NM_001127639
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCAGGTTCATAGGACTCTTCCTATTTCAGGCCATATCTTGGGCATATGGTGCCCAACCCTGCA
  TCCCCAAAAGTTTTGGCTACAGCTCAGTGGTCTGTGTCTGCAATGCAACGTACTGTGACTCTCTTGACCC
  CCTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTATGAGAGTACTCGAAGCGGACGTCGGATGGAGCTG
  AGTACCGGGTCCATCCAGGCCAACCGCACTGGCACAGGGCTCCTACTAACCTTGCAGCCAGAAGAGAAGT
  TCCAGAAAGTGAAAGGATTTGGAGGTGCCATGACAGATGCCACTGCGCTCAACATCCTTGCCTTGTCCCC
  CCCGGCTCAGAAACTGCTACTCAAGTCCTACTTCTCTAGCGAAGGCATTGAGTATAACATCATCCGGGTA
  CCCATGGCCAGTTGTGACTTCTCCATCCGCATCTACACCTATGCTGACACCCCTAATGACTTCCAGTTAT
  CCAACTTCAGCCTCCCGGAGGAAGACACCAAGCTCAAGATACCCCTGATTCACCGAGCCCTGAAGATGTC
  CCCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAGCAGTG
  AATGGGAAAGGGTCACTCAAGGGTCACCCAGGGGATATCTATCACGAGGCCTGGGCCAATTACTTTGTCA
  AGTTTCTGGATGCTTATGCTACACACAACATCAAATTCTGGGCAGTGACAGCGGAGAATGAACCCTCTGC
  AGGGCTCTTCACTGGGTACCCCTTCCAATGCCTGGGCTTCACTGCTGAACATCAGAGAGACTTTATTTCC
  CATGATCTAGGGCCAGCCCTTGCCAACAGTTCTCATGATGTGAAGCTACTCATACTGGATGATCAGCGGC
  TGCTGCTACCCCGCTGGGCACAGGTGGTGCTCTCTGACCCAGAGGCAGCCAAGTACGTTCATGGCATCGC
  TGTCCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC
  AACATGATGCTCTTTGCTTCAGAGGCCTGCGTGGGCTCCAAGTTCTGGGAGCAGAGTGTTCGGTTAGGCT
  CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC
  TGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTCGTTGATAGCCCCATC
  ATTGTCGACATCCCCAAAGACACATTTTACAAGCAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT
  TCATCCCTGAGGGATCTCAGCGAGTGGGCTTGGTGGCCAGTGAGAAGACCGACTTGGAAACAGTGGCACT
  GATACGCCCTGATGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCGTCTAAGGATGTCCCTCTTACCATC
  AGTGATCCTGCCCTGGGCTTCATGGAGACCATCTCACCTGGCTACTCCATTCACACTTACCTATGGCGTC
  GCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001127639
• Insert Size: 1548 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127639.1, NP_001121111.1
• RefSeq Size: 2159 bp
• RefSeq ORF: 1548 bp
• Locus ID: 684536
• Cytogenetics: 2q34