Gba (NM_001127639) Rat Tagged ORF Clone

CAT#: RR205005

Gba (Myc-DDK-tagged ORF) - Rat glucosidase, beta, acid (Gba), (10 ug)

Product Images

Circular map for RR205005

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gba
• Synonyms: MGC188451
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR205005 representing NM_001127639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCAGGTTCATAGGACTCTTCCTATTTCAGGCCATATCTTGGGCATATGGTGCCCAACCCTGCA
  TCCCCAAAAGTTTTGGCTACAGCTCAGTGGTCTGTGTCTGCAATGCAACGTACTGTGACTCTCTTGACCC
  CCTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTATGAGAGTACTCGAAGCGGACGTCGGATGGAGCTG
  AGTACCGGGTCCATCCAGGCCAACCGCACTGGCACAGGGCTCCTACTAACCTTGCAGCCAGAAGAGAAGT
  TCCAGAAAGTGAAAGGATTTGGAGGTGCCATGACAGATGCCACTGCGCTCAACATCCTTGCCTTGTCCCC
  CCCGGCTCAGAAACTGCTACTCAAGTCCTACTTCTCTAGCGAAGGCATTGAGTATAACATCATCCGGGTA
  CCCATGGCCAGTTGTGACTTCTCCATCCGCATCTACACCTATGCTGACACCCCTAATGACTTCCAGTTAT
  CCAACTTCAGCCTCCCGGAGGAAGACACCAAGCTCAAGATACCCCTGATTCACCGAGCCCTGAAGATGTC
  CCCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAATGGAGCAGTG
  AATGGGAAAGGGTCACTCAAGGGTCACCCAGGGGATATCTATCACGAGGCCTGGGCCAATTACTTTGTCA
  AGTTTCTGGATGCTTATGCTACACACAACATCAAATTCTGGGCAGTGACAGCGGAGAATGAACCCTCTGC
  AGGGCTCTTCACTGGGTACCCCTTCCAATGCCTGGGCTTCACTGCTGAACATCAGAGAGACTTTATTTCC
  CATGATCTAGGGCCAGCCCTTGCCAACAGTTCTCATGATGTGAAGCTACTCATACTGGATGATCAGCGGC
  TGCTGCTACCCCGCTGGGCACAGGTGGTGCTCTCTGACCCAGAGGCAGCCAAGTACGTTCATGGCATCGC
  TGTCCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACACACCGCTTGTTCCCC
  AACATGATGCTCTTTGCTTCAGAGGCCTGCGTGGGCTCCAAGTTCTGGGAGCAGAGTGTTCGGTTAGGCT
  CCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTACCACGTAACTGGATGGAC
  TGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAACTTCGTTGATAGCCCCATC
  ATTGTCGACATCCCCAAAGACACATTTTACAAGCAGCCCATGTTCTACCATCTTGGCCACTTCAGCAAGT
  TCATCCCTGAGGGATCTCAGCGAGTGGGCTTGGTGGCCAGTGAGAAGACCGACTTGGAAACAGTGGCACT
  GATACGCCCTGATGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCGTCTAAGGATGTCCCTCTTACCATC
  AGTGATCCTGCCCTGGGCTTCATGGAGACCATCTCACCTGGCTACTCCATTCACACTTACCTATGGCGTC
  GCCAGGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR205005 representing NM_001127639
  Red=Cloning site Green=Tags(s)
  
  MAARFIGLFLFQAISWAYGAQPCIPKSFGYSSVVCVCNATYCDSLDPLTLPALGTFSRYESTRSGRRMEL
  STGSIQANRTGTGLLLTLQPEEKFQKVKGFGGAMTDATALNILALSPPAQKLLLKSYFSSEGIEYNIIRV
  PMASCDFSIRIYTYADTPNDFQLSNFSLPEEDTKLKIPLIHRALKMSPRPISLFASPWTSPTWLKTNGAV
  NGKGSLKGHPGDIYHEAWANYFVKFLDAYATHNIKFWAVTAENEPSAGLFTGYPFQCLGFTAEHQRDFIS
  HDLGPALANSSHDVKLLILDDQRLLLPRWAQVVLSDPEAAKYVHGIAVHWYMDFLAPAKATLGETHRLFP
  NMMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVTGWTDWNLALNPEGGPNWVRNFVDSPI
  IVDIPKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASEKTDLETVALIRPDGSAVVVVLNRSSKDVPLTI
  SDPALGFMETISPGYSIHTYLWRRQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127639
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127639.1, NP_001121111.1
• RefSeq Size: 2159 bp
• RefSeq ORF: 1548 bp
• Locus ID: 684536
• Cytogenetics: 2q34
• MW: 57.5 kDa