Dicer (DICER1) (NM_177438) Human Tagged ORF Clone

CAT#: RG219214

DICER1 (tGFP-tagged) - Human dicer 1, ribonuclease type III (DICER1), transcript variant 1

Product Images

Circular map for RG219214

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dicer
• Synonyms: DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219214 representing NM_177438
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAGCCCTGCTTTGCAACCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCAA
  TGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAATA
  TCAGGTTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGGAAG
  ACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCAGAAATGGAA
  AAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCTGTCAGAACTCATTC
  AGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGGAACCAA
  GAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAATGGTTACT
  TATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCACCCCTATCG
  AGAAATTATGAAGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCTTCCATTTTA
  AATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTTAAGAGTAATG
  CTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCATGTGAGATTGTGGTGGATTG
  TGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGCTGATGGAATTAGAAGAAGCACTTAATTTT
  ATCAATGATTGTAATATATCTGTACATTCAAAAGAAAGAGATTCTACTTTAATTTCGAAACAGATACTAT
  CAGACTGTCGTGCCGTATTGGTAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCTGGAATGATGGTAAG
  AGAACTACAGAAATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTATTGTTTACAGACACT
  TTCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTTGACCTGAAATTTGTAA
  CTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATGAGCGACAGCAGTTTGAAAG
  CGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGTCATGGAGTGATTCTGAGGATGATGATGAG
  GATGAAGAAATTGAAGAAAAAGAGAAGCCAGAGACAAATTTTCCTTCTCCTTTTACCAACATTTTGTGCG
  GAATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCTTAAACAGATTGATAAAGGAAGCTGGCAAACA
  AGATCCAGAGCTGGCTTATATCAGTAGCAATTTCATAACTGGACATGGCATTGGGAAGAATCAGCCTCGC
  AACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGGAAATTTCGAGCACATGAGACCA
  ACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCAAAATGCAACTTGGTGGTTCGTTT
  TGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAGGAAGAGCAAGGGCACCCATCTCTAATTAT
  ATAATGTTAGCGGATACAGACAAAATAAAAAGTTTTGAAGAAGACCTTAAAACCTACAAAGCTATTGAAA
  AGATCTTGAGAAACAAGTGTTCCAAGTCGGTTGATACTGGTGAGACTGACATTGATCCTGTCATGGATGA
  TGATGACGTTTTCCCACCATATGTGTTGAGGCCTGACGATGGTGGTCCACGAGTCACAATCAACACGGCC
  ATTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGATCCGTTTACTCATCTAGCTCCTAAATGCA
  GAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTTTATCTGCCAATTAACTCACCTCTTCGAGC
  CTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCTGAAAGAGTTGTAGCTCTCATTTGCTGTGAG
  AAACTGCACAAAATTGGCGAACTGGATGACCATTTGATGCCAGTTGGGAAAGAGACTGTTAAATATGAAG
  AGGAGCTTGATTTGCATGATGAAGAAGAGACCAGTGTTCCAGGAAGACCAGGTTCCACGAAACGAAGGCA
  GTGCTACCCAAAAGCAATTCCAGAGTGTTTGAGGGATAGTTATCCCAGACCTGATCAGCCCTGTTACCTG
  TATGTGATAGGAATGGTTTTAACTACACCTTTACCTGATGAACTCAACTTTAGAAGGCGGAAGCTCTATC
  CTCCTGAAGATACCACAAGATGCTTTGGAATACTGACGGCCAAACCCATACCTCAGATTCCACACTTTCC
  TGTGTACACACGCTCTGGAGAGGTTACCATATCCATTGAGTTGAAGAAGTCTGGTTTCATGTTGTCTCTA
  CAAATGCTTGAGTTGATTACAAGACTTCACCAGTATATATTCTCACATATTCTTCGGCTTGAAAAACCTG
  CACTAGAATTTAAACCTACAGACGCTGATTCAGCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTC
  CAGCACTTTGGATATTGACTTTAAATTCATGGAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCC
  AGTACAAAGTATACAAAAGAAACACCCTTTGTTTTTAAATTAGAAGATTACCAAGATGCCGTTATCATTC
  CAAGATATCGCAATTTTGATCAGCCTCATCGATTTTATGTAGCTGATGTGTACACTGATCTTACCCCACT
  CAGTAAATTTCCTTCCCCTGAGTATGAAACTTTTGCAGAATATTATAAAACAAAGTACAACCTTGACCTA
  ACCAATCTCAACCAGCCACTGCTGGATGTGGACCACACATCTTCAAGACTTAATCTTTTGACACCTCGAC
  ATTTGAATCAGAAGGGGAAAGCGCTTCCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCT
  GCAGAATAAACAGATACTGGTTCCAGAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAA
  GCTGTTTGTCTCCCCAGCATACTTTATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAAGAGCCCAGA
  CTGCCAGCGATGCTGGCGTGGGAGTCAGATCACTTCCTGCGGATTTTAGATACCCTAACTTAGACTTCGG
  GTGGAAAAAATCTATTGACAGCAAATCTTTCATCTCAATTTCTAACTCCTCTTCAGCTGAAAATGATAAT
  TACTGTAAGCACAGCACAATTGTCCCTGAAAATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAG
  AAAATCATGACCAAATGTCTGTGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGA
  AGTTTCAGCAGATCTTACAGCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGAT
  TTAGCTAACAGAGACTTTTGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCGTGCAACCAA
  CTACCTCATATTCCATTCAGAATTTATACAGTTACGAGAACCAGCCCCAGCCCAGCGATGAATGTACTCT
  CCTGAGTAATAAATACCTTGATGGAAATGCTAACAAATCTACCTCAGATGGAAGTCCTGTGATGGCCGTA
  ATGCCTGGTACGACAGACACTATTCAAGTGCTCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTG
  GGTACTCCTCAAGGACTCTTGGCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAG
  TGATGGATTTAACCTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATAT
  CTATTTTGCACTTACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACT
  GTAATCTGTATCGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGGTGTCAATATTTGATCCCCC
  TGTGAATTGGCTTCCTCCTGGTTATGTAGTAAATCAAGACAAAAGCAACACAGATAAATGGGAAAAAGAT
  GAAATGACAAAAGACTGCATGCTGGCGAATGGCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGG
  AGGAGAGCCTGATGTGGAGGGCTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCA
  GGAACATATCAGATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCT
  CCTTTTTCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTAGTATGCCAT
  TTTCATCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCTATCTGGATCCTAGCAAAGC
  TGTTGAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCATCAGAAGAAAACTGTGGTGTTGACACGGGA
  AAGCAGTCCATTTCTTACGACTTGCACACTGAGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGG
  AAGCCCTGCTGGGCTGCTATTTAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGG
  GCTGAAGGTGCTCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTC
  AACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCTCTTCTGTAT
  TGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTGATCATCCAGATGCAGATAA
  AACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAAAGAAAATCAACTACAGATTCAAGAATAAG
  GCTTACCTTCTCCAGGCTTTTACACATGCCTCCTACCACTACAATACTATCACTGATTGTTACCAGCGCT
  TAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGGCAGCA
  CTCCCCGGGGGTCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTGGCTGTA
  AAGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATGACTTTGTGC
  AGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGAGATCTGAGGAGGATGAAGA
  GAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATATTTTTGAGTCGCTTGCTGGTGCCATTTAC
  ATGGATAGTGGGATGTCACTGGAGACAGTCTGGCAGGTGTACTATCCCATGATGCGGCCACTAATAGAAA
  AGTTTTCTGCAAATGTACCCCGTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAAACTGCCAAATT
  TAGCCCGGCTGAGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGAAAGGGGAAATTT
  AAAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTCCGAAGCCTCAAAG
  CTAATCAACCTCAGGTTCCCAATAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219214 representing NM_177438
  Red=Cloning site Green=Tags(s)
  
  MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGK
  TFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQ
  EFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASIL
  NGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNF
  INDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDT
  FLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDE
  DEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPR
  NKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNY
  IMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTA
  IGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCE
  KLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYL
  YVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSL
  QMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIP
  STKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDL
  TNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRK
  AVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDN
  YCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYD
  LANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAV
  MPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTY
  LFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKD
  EMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLS
  PFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTG
  KQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENF
  NSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNK
  AYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAV
  KYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIY
  MDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKF
  KGVGRSYRIAKSAAARRALRSLKANQPQVPNS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177438
• ORF Size: 5766 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177438.3
• RefSeq Size: 10276 bp
• RefSeq ORF: 5769 bp
• Locus ID: 23405
• UniProt ID: Q9UPY3
• Cytogenetics: 14q32.13
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]