GCKR (NM_001486) Human Tagged ORF Clone

CAT#: RG214230

GCKR (tGFP-tagged) - Human glucokinase (hexokinase 4) regulator (GCKR)

Product Images

Circular map for RG214230

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GCKR
• Synonyms: FGQTL5; GKRP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214230 representing NM_001486
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGCACAAAACGGTTTCAACATGTCATTGAGACCCCGGAGCCTGGCAAGTGGGAGTTGTCTGGGT
  ACGAGGCAGCTGTGCCAATCACGGAGAAGTCAAACCCACTGACCCAGGATCTAGACAAAGCAGATGCTGA
  GAACATTGTTCGACTGCTAGGGCAATGTGATGCTGAGATCTTCCAGGAGGAGGGGCAAGCCCTGTCCACA
  TACCAGAGACTCTACAGCGAATCCATTCTGACCACCATGGTACAGGTGGCTGGGAAAGTTCAGGAAGTGC
  TGAAGGAGCCAGATGGGGGGCTGGTTGTGCTGAGTGGAGGGGGCACCTCTGGCCGGATGGCATTCCTCAT
  GTCGGTGTCCTTTAATCAGCTGATGAAAGGTCTGGGACAGAAACCTCTTTACACCTACCTCATTGCAGGT
  GGTGACAGGTCTGTGGTGGCCTCTAGGGAGGGGACAGAAGATAGTGCCTTGCACGGGATTGAGGAACTGA
  AGAAGGTGGCTGCCGGGAAGAAGAGAGTGATTGTCATTGGCATTTCTGTGGGACTCTCTGCTCCCTTTGT
  GGCAGGCCAGATGGACTGCTGCATGAACAACACAGCTGTCTTCTTGCCAGTCCTGGTTGGCTTCAATCCA
  GTGAGCATGGCCAGAAATGACCCCATTGAAGACTGGAGTTCAACATTCCGACAAGTAGCAGAGCGGATGC
  AGAAAATGCAGGAGAAACAGAAAGCTTTTGTGCTCAATCCTGCCATCGGGCCCGAGGGTCTCAGCGGCTC
  CTCCCGGATGAAAGGTGGAAGTGCCACCAAGATTCTGCTGGAAACCCTGTTATTAGCAGCCCATAAGACT
  GTGGACCAGGGCATTGCAGCATCTCAAAGATGCCTCCTGGAAATCTTGCGGACATTTGAGCGAGCTCATC
  AGGTGACCTACAGCCAAAGCCCCAAGATTGCCACCCTGATGAAGAGTGTCAGCACCAGTCTGGAGAAGAA
  AGGCCACGTGTACCTGGTTGGCTGGCAGACCCTGGGCATCATTGCCATCATGGATGGAGTAGAGTGCATC
  CACACCTTTGGTGCTGATTTCCGAGATGTCCGTGGCTTTCTCATTGGTGATCACAGTGACATGTTTAACC
  AGAAGGCTGAGCTCACCAACCAGGGTCCCCAGTTCACCTTCTCCCAGGAGGACTTCCTGACTTCCATCCT
  TCCCTCTCTCACGGAAATCGATACTGTGGTCTTCATTTTCACCCTGGATGACAACCTCACGGAGGTGCAG
  ACTATAGTGGAGCAGGTGAAAGAGAAGACCAACCACATCCAGGCCCTGGCACACAGCACCGTGGGTCAGA
  CCTTGCCGATCCCTCTGAAGAAGCTCTTTCCCTCCATCATCAGCATCACATGGCCACTGCTTTTCTTTGA
  ATATGAAGGGAACTTCATCCAGAAGTTCCAGCGTGAGCTAAGCACCAAATGGGTGCTGAATACAGTGAGT
  ACAGGTGCTCATGTGCTTCTTGGTAAGATCCTACAAAACCACATGTTGGACCTTCGGATTAGCAACTCCA
  AGCTCTTCTGGCGGGCGCTGGCCATGCTGCAGCGGTTCTCTGGACAGTCCAAGGCTCGATGCATCGAGAG
  CCTCCTCCGAGCGATCCACTTTCCCCAGCCACTGTCAGATGATATTCGGGCTGCTCCCATCTCCTGCCAT
  GTCCAGGTTGCACATGAGAAGGAACAGGTGATACCCATCGCCTTGCTGAGCCTCCTATTCCGGTGCTCGA
  TCACTGAGGCTCAGGCACACCTGGCTGCAGCTCCTTCTGTCTGTGAGGCTGTCAGGAGTGCTCTTGCTGG
  GCCAGGTCAGAAGCGCACTGCGGACCCCCTCGAGATCCTAGAGCCTGACGTTCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214230 representing NM_001486
  Red=Cloning site Green=Tags(s)
  
  MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALST
  YQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAG
  GDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNP
  VSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKT
  VDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECI
  HTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQ
  TIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVS
  TGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCH
  VQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001486
• ORF Size: 1875 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001486.2, NP_001477.1
• RefSeq Size: 2197 bp
• RefSeq ORF: 1878 bp
• Locus ID: 2646
• UniProt ID: Q14397
• Cytogenetics: 2p23.3
• Domains: SIS
• Gene Summary: This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY). [provided by RefSeq, Jul 2008]